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Comprehensive analysis of alternative splicing variants identified from tonotopical differences in the mouse cochlea

Research Project

Project/Area Number 25670715
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Otorhinolaryngology
Research InstitutionShinshu University

Principal Investigator

YOSHIMURA Hidekane  信州大学, 医学部, 助教(特定雇用) (10612997)

Co-Investigator(Renkei-kenkyūsha) NISHIO Shin-ya  信州大学, 学術研究院医学系, 助教 (70467166)
USAMI Shin-ichi  信州大学, 学術研究院医学系, 教授 (10184996)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords内耳 / 遺伝子発現 / 難聴 / 耳科学 / 聴覚 / 遺伝子 / マイクロアレイ
Outline of Final Research Achievements

The mammalian cochlea is a spiral shaped auditory sensing organ, with the mouse cochlea forming 2.5 turns around its axis. This cochlear structure has an important role in the mechanism for distinguishing pitch (sound frequency). This mechanism were based on the synchronized vibration of the basilar membrane is thought to be caused by differences in the thickness and width of the basilar membrane. In this study, we performed exon level gene expression analysis using the previous cDNA microarray data to identify alternative splicing variants in specific regions of the cochlea. As a result, we identified cochlear turn specific alternative splicing variants in Otog, Strc, Tectb and Slc26a4 genes. This dataset will provide a valuable base for understanding the detailed mechanisms not only for specific frequency deterioration in cases of hearing loss but also those for normal hearing.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (7 results)

All 2016 2015 2014

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (2 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Identification of a Novel CLRN1 Gene Mutation in Usher Syndrome Type 3: Two Case Reports.2015

    • Author(s)
      Yoshimura H, Oshikawa C, Nakayama J, Moteki H, Usami SI.
    • Journal Title

      Ann Otol Rhinol Laryngol

      Volume: 124

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel ABHD12 Mutations in PHARC Patients: The Differential Diagnosis of Deaf-Blindness.2015

    • Author(s)
      Yoshimura H, Hashimoto T, Murata T, Fukushima K, Sugaya A, Nishio SY, Usami SI.
    • Journal Title

      Ann Otol Rhinol Laryngol

      Volume: 124

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] USH2 Caused by GPR98 Mutation Diagnosed by Massively Parallel Sequencing in Advance of the Occurrence of Visual Symptoms.2015

    • Author(s)
      Moteki H, Yoshimura H, Azaiez H, Booth KT, Shearer AE, Sloan CM, Kolbe DL, Murata T, Smith RJ, Usami SI.
    • Journal Title

      Ann Otol Rhinol Laryngol

      Volume: 124

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Massively parallel DNA sequencing facilitates diagnosis of patients with Usher syndrome type 12014

    • Author(s)
      Yoshimura H, Iwasaki S, Nishio SY, Kumakawa K, Tono T, Kobayashi Y, Sato H, Nagai K, Ishikawa K, Ikezono T, Naito Y, Fukushima K, Oshikawa C, Kimitsuki T, Nakanishi H, Usami S.
    • Journal Title

      PLoS One

      Volume: 11 Issue: 3 Pages: e90688-e90688

    • DOI

      10.1371/journal.pone.0090688

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Deafness gene expression patterns in the mouse cochlea found by microarray analysis.2014

    • Author(s)
      Yoshimura H, Takumi Y, Nishio SY, Suzuki N, Iwasa Y, Usami S.
    • Journal Title

      PLoS One.

      Volume: 9 Issue: 3 Pages: e92547-e92547

    • DOI

      10.1371/journal.pone.0092547

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Laser-capture micro dissection combined with next-generation sequencing analysis of cell type-specific deafness gene expression in the mouse cochlea.2016

    • Author(s)
      Nishio S, Takumi Y, Yoshimura H, Usami S.
    • Organizer
      ARO 39th MidWinter Metting
    • Place of Presentation
      San Diego, California, USA
    • Year and Date
      2016-02-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Microarray Analysis of Tonotopic Gene Expression Patterns in the Mouse Cochlea2014

    • Author(s)
      Yosimura H, Nishio S, Takumi Y, Iwasa Y, Usami S.
    • Organizer
      Inner Ear Biology Workshop
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2014-11-01 – 2014-11-04
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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