| Project/Area Number |
25670720
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Nara Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OUJI Yukiteru 奈良県立医科大学, 医学部, 講師 (50343421)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 内耳 / 再生医学 / 有毛細胞 / 発生 / Wnt / 細胞培養 / 器官培養 / Wntシグナル / 耳科学 |
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| Outline of Final Research Achievements |
To establish the in vitro system recapturing inner ear development is needed for the evaluation of cellular dynamics related to inner ear. In this study, we developed long-term cultivation method of the otic vesicle cells (OVCs) isolated from mouse embryos. OVCs were successfully maintained in the undifferentiated state judging from the confirmed expression of nestin and Oct-3/4. OVCs were transfected with the GFP-expressing plasmid under the control of Math1 promoter region, and the M-OVC cells, which become to express GFP with Math1, were obtained. One Wnt protein among various Wnts was found to promote the differentiation of M-OVCs into hair cell-like cells. Furthermore, it was observed that the co-culture with a certain cell line as effector cells could affect the differentiation of M-OVCs. These results strongly suggest the important roles of the specific Wnt and effector cells on the regulation in the differentiation of OVC.
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