In vitro system recapturing inner ear development using otic vesicle cells, Wnts and effector cells
Project/Area Number |
25670720
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Otorhinolaryngology
|
Research Institution | Nara Medical University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
OUJI Yukiteru 奈良県立医科大学, 医学部, 講師 (50343421)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 内耳 / 再生医学 / 有毛細胞 / 発生 / Wnt / 細胞培養 / 器官培養 / Wntシグナル / 耳科学 |
Outline of Final Research Achievements |
To establish the in vitro system recapturing inner ear development is needed for the evaluation of cellular dynamics related to inner ear. In this study, we developed long-term cultivation method of the otic vesicle cells (OVCs) isolated from mouse embryos. OVCs were successfully maintained in the undifferentiated state judging from the confirmed expression of nestin and Oct-3/4. OVCs were transfected with the GFP-expressing plasmid under the control of Math1 promoter region, and the M-OVC cells, which become to express GFP with Math1, were obtained. One Wnt protein among various Wnts was found to promote the differentiation of M-OVCs into hair cell-like cells. Furthermore, it was observed that the co-culture with a certain cell line as effector cells could affect the differentiation of M-OVCs. These results strongly suggest the important roles of the specific Wnt and effector cells on the regulation in the differentiation of OVC.
|
Report
(3 results)
Research Products
(11 results)
-
-
[Journal Article] Cytotoxic human peripheral blood-derived γδT cells kill glioblastoma cell lines: implications for cell-based immunotherapy for patients with glioblastoma2014
Author(s)
Nakazawa T, Nakamura M, Park YS, Motoyama Y, Hironaka Y, Nishimura F, Nakagawa I, Yamada S, Matsuda R, Tamura K, Sugimoto T, Takeshima Y, Marutani A, Tsujimura T, Ouji N, Ouji Y, Yoshikawa M, Nakase H
-
Journal Title
J Neurooncol
Volume: 116
Issue: 1
Pages: 31-39
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-