| Project/Area Number |
25670721
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Otorhinolaryngology
|
|---|
| Research Institution | Juntendo University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAMIYA Kazusaku 順天堂大学, 医学部, 准教授 (10374159)
IIZUKA Takashi 順天堂大学, 医学部, 助教 (40372932)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 聴覚障害 / 人工多能性幹細胞 / 内耳 / 有毛細胞 / ラセン神経節細胞 / 分化誘導 / 細胞治療 / 再生医療 / 遺伝性難聴 / iPS細胞 / 内耳前駆細胞 / 多能性幹細胞 / 幹細胞ホーミング / 内耳有毛細胞 / 蝸牛線維細胞 |
|---|
| Outline of Final Research Achievements |
In this study, we co-cultured iPS-derived cells with three different regions of the cochlear tissue and induced their differentiation into various types of inner cells. The number of Nanog-positive colonies with reporter GFP controlled by Nanog promotor was counted to assess the undifferentiated level of the cells. In the co-cultured cells with spiral ganglion, many cells with neurite-like protrusions were observed among the colonies. In the co-culture with the organ of Corti and spiral limbus (OC/SL), neuron-like cells were observed on the cell layer with tight junction-like adhesions. In the co-culture with the cochlear lateral wall, many cell layers with tight junction-like adhesions were observed, while undifferentiated Nanog-positive colonies were not observed. It was suggested that differentiation of iPS cells to cochlear cells was regulated depending on the part of the co-cultured organs.
|
