New method for auditory nerve stimulation using optogenetics

• Project/Area Number: 25670722
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: National Rehabilitation Center for Persons with Disabilities
• Principal Investigator: Takago Hideki 国立障害者リハビリテーションセンター(研究所), 研究所感覚機能系障害研究部, 研究室長 (70401354)
• Co-Investigator(Kenkyū-buntansha): OSHIMA Tomoko (TAKAGO Tomoko) 国立障害者リハビリテーションセンター, 研究所感覚機能系障害研究部, 流動研究員 (50731783)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 光遺伝学 / オプトジェネティクス / 人工内耳 / 蝸牛 / 蝸牛神経 / パッチクランプ / 国際情報交換（ドイツ） / 有毛細胞

Outline of Final Research Achievements

Heterogeneity of excitatory postsynaptic currents in the mammalian auditory nerve fibers is the hallmark of cochlear physiology. In the present study, we found that uniquantal release of neurotransmitter glutamate through dynamic fusion pore controls the exocytosis at the inner hair cell afferent synapse. Furthermore we found that otoferlin (a causative gene for hereditary deafness DFNB9) plays an essential role for the heterogeneity of synaptic currents at this synapse.

Research Products

• [Int'l Joint Research] ゲッティンゲン大学(ドイツ)
• [Journal Article] Developmental increase in hyperpolarization-activated current regulates intrinsic firing properties in rat vestibular ganglion cells (2015)
  • Author(s): Yoshimoto R, Iwasaki S, Takago H, Nakajima T, Sahara Y, Kitamura K
  • Journal Title: Neuroscience Volume: 284 Pages: 632-642
  • DOI: 10.1016/j.neuroscience.2014.10.034
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Uniquantal release through a dynamic fusion pore is a candidate mechanism of hair cell exocytosis (2014)
  • Author(s): Chapochnikov NM*, Takago H*, Huang CH, Pangrsic T, Khimich D, Neef J, Auge E, Goettfert F, Hell S, Wichmann C, Wolf F, Moser T (* Co-1st authors)
  • Journal Title: Neuron Volume: 83 Pages: 1389-1403
  • DOI: 10.1016/j.neuron.2014.08.003
  • Peer Reviewed / Open Access
• [Presentation] マウス内有毛細胞リボンシナプスにおけるOtoferlinによる神経伝達物質放出の制御 (2015)
  • Author(s): 鷹合 秀輝
  • Organizer: 第116回日本耳鼻咽喉科学会総会・学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2015-05-21
• [Presentation] Otoferlinはマウス内有毛細胞リボンシナプスにおいてエキソサイトーシスのモードを変える (2015)
  • Author(s): 鷹合 秀輝, 大島－鷹合 知子, トビアス モーザー
  • Organizer: 第92回日本生理学会大会
  • Place of Presentation: 神戸国際会議場・展示場
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Otoferlin alters mode of exocytosis at the inner hair cell ribbon synapse (2014)
  • Author(s): Hideki Takago, Tobias Moser
  • Organizer: The 1st annual meeting of the society for bioacoustics
  • Place of Presentation: Doshisha Biwako Retreat Center
  • Year and Date: 2014-12-13 – 2014-12-14
• [Presentation] Evaluation of synaptic function of otoferlin at the mouse inner hair cell ribbon synapse by postsynaptic recording (2014)
  • Author(s): Hideki Takago, Tobias Moser
  • Organizer: Inner Ear Biology 2014 in Kyoto
  • Place of Presentation: Kyoto International Conference Center
  • Year and Date: 2014-11-01 – 2014-11-04
• [Presentation] マウス内有毛細胞リボンシナプスにおける神経伝達物質放出様式 (2014)
  • Author(s): 鷹合 秀輝
  • Organizer: 第115回日本耳 鼻咽喉科学会総会・学術講演会
  • Place of Presentation: ヒルトン福岡シーホーク
  • Year and Date: 2014-05-15 – 2014-05-17
• [Presentation] マウス内有毛細胞リボンシナプスにおけるBassoonとシナプスリボンによるシナプス伝達の制御
  • Author(s): 鷹合秀輝
  • Organizer: 第114回日本耳鼻咽喉科学会総会・学術講演会
  • Place of Presentation: ロイトン札幌（札幌市）
• [Presentation] マウス内有毛細胞求心性シナプスにおけるotoferlinの機能の分析
  • Author(s): 鷹合秀輝, Tobias Moser
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島市）