Analysis of expression of Cd antigens in retinoblastoma, and its application for disease classification and therapeutic strategy

• Project/Area Number: 25670726
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: The University of Tokyo
• Principal Investigator: SUMIKO watanabe 東京大学, 医科学研究所, 特任教授 (60240735)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 網膜 / 腫瘍 / 表面抗原 / 網膜芽細胞腫 / FACS / ケモカイン

Outline of Final Research Achievements

Retinoblastoma is the most frequent cancer in the eye. Retinoblastoma showed different pathological types, but there is no classification based on knowledge of gene information such as mutation, or translocation. Cell surface antigens are proteins that expressed in cell surface, and can be used for identification and purification of certain subset of cells. We analyzed expression pattern of Cd antigens in patient derived retinoblastoma clinical samples, and found that different expression patterns of Cd antigens in different pathological featured retinoblastoma. These results suggested information of expression pattern of Cd antigens can be used for diagnosis and planning of therapeutic strategy,

Research Products

• [Journal Article] Gene activated-matrix comprised of atelocollagen and plasmid DNA encoding Bmp4 or Runx2 promotes rat cranial bone augmentation (2015)
  • Author(s): Umebayashi M., Sumita, Y., Kawai, Y., Watanabe, S., Asahina, I.
  • Journal Title: Bio Research Open Access Volume: -
  • Peer Reviewed / Open Access
• [Journal Article] Ablation of Kcnj10 expression in retinal explants revealed pivotal roles for Kcnj10 in the proliferation and development of Muller glia (2015)
  • Author(s): Arai, E., Baba, Y., Iwagawa, T.,Kuribayashi, H., Mochizuki, Y., Watanabe, S.
  • Journal Title: Mol Vis Volume: 21 Pages: 148-159
  • Peer Reviewed
• [Journal Article] Roles Histone H3K27 tri-methylase Ezh2 in retinal proliferation and differentiation (2015)
  • Author(s): ida, A., Iwagawa, T., Baba, Y.,Satoh, S., Mochizuki, Y., Nakauchi, H., Furukawa, T., Koseki, H., Murakami, A., Watanabe, S.
  • Journal Title: Developmental Neurobiology Volume: - Issue: 9 Pages: 3751-3756
  • DOI: 10.1002/dneu.22261
  • Peer Reviewed
• [Journal Article] DNA methylation is involved in the expression of miR-142-3p in fibroblasts and induced pluripotent stem cells (2014)
  • Author(s): Abdul Razak, S. A., Baba, Y., Nakauchi, H., Otsu, M., Watanabe, S.
  • Journal Title: Stem Cells International Volume: 101349 Pages: 1-8
  • DOI: 10.1155/2014/101349
  • Peer Reviewed
• [Journal Article] Critical Roles of DNase1l3l in Lens Nuclear Degeneration in Zebrafish (2014)
  • Author(s): Iida, A., Tabata, Y., Baba, Y., Fujii, T., Watanabe, S.
  • Journal Title: Biochimie Volume: 106 Pages: 68-74
  • DOI: 10.1016/j.biochi.2014.07.025
  • Peer Reviewed
• [Journal Article] Jmjd3 is required for the development of subsets of retinal bipolar cells (2014)
  • Author(s): Iida, A., Iwagawa, T., Kuribayashi, H., Satoh. S., Mochizuki, Y., Baba, Y., Nakauchi, H., Furukawa, T, Koseki, H, Murakami, A., Watanabe, S.
  • Journal Title: Proc.Natl.Acad.Sci.,USA Volume: 111 Pages: 3751-3756
  • Peer Reviewed
• [Journal Article] BMP signaling participates in late phase differentiation of the retina, party via upregulation of Hey2 (2014)
  • Author(s): Kuribayashi, H., Baba, Y., Watanabe, S.
  • Journal Title: Developmental Neurobiology Volume: 74 Issue: 12 Pages: 1172-1183
  • DOI: 10.1002/dneu.22196
  • Peer Reviewed
• [Journal Article] Transposon mutagenesis identifies Foxr2 as a putative oncogene in medulloblastoma (2014)
  • Author(s): Koso, H., Tsuhako, A., Lyons, E., Ward, J. M., Rust, A. G., Adams, D. J., Jenkins, N. A., Copeland, N. G., Watanabe, S.
  • Journal Title: Can Res Volume: 74 Pages: 2351-2361
  • Peer Reviewed
• [Journal Article] Use of cell type-specific transcriptome to identify genes specifically involved in Müller glia differentiation during retinal development. (2014)
  • Author(s): Mochizuki Y, Iida A, Lyons E, Kageyama R, Nakauchi H, Murakami A, Watanabe S.
  • Journal Title: Dev Neurobiol. Volume: 74 Issue: 4 Pages: 426-37
  • DOI: 10.1002/dneu.22131
  • Peer Reviewed
• [Journal Article] Jmjd3 is required for the development of subsets of retinal bipolar cells (2014)
  • Author(s): Iida, A. et al.
  • Journal Title: Proc.Natl. Acad. Sci., USA Volume: 111 Pages: 3751-3756
  • Peer Reviewed
• [Journal Article] Use of cell type-specific transcriptome to identify genes specifically involved in Muller glia differentiation during retinal development (2013)
  • Author(s): Mochizuki, Y. et al.
  • Journal Title: Dev Neurobiol Volume: Nov4
  • Peer Reviewed
• [Journal Article] The early retinal progenitor-expressed gene Sox11 regulates the timing of the differentiation of retinal cells (2013)
  • Author(s): Usui, A. et al.
  • Journal Title: Development Volume: 140 Pages: 740-750
  • Peer Reviewed
• [Journal Article] Robust long-term transduction of common marmoset neuromuscular tissue with rAAV1 and rAAV9 (2013)
  • Author(s): Okada, H. et al.
  • Journal Title: Molecular Therapy - Nucleic Acids Volume: 2
  • Peer Reviewed
• [Journal Article] (2013) Enhancer/promoter activities of the long/middle wavelength-sensitive opsins of vertebrates mediated by thyroid hormone receptor β2 and COUP-TFII (2013)
  • Author(s): Iwagawa, T et al.
  • Journal Title: Plos One Volume: 8
  • Peer Reviewed
• [Journal Article] Profiling of MicroRNA in Human and Mouse ES and iPS Cells Reveals Overlapping but Distinct MicroRNA Expression Patterns (2013)
  • Author(s): Siti Razila Abdul Razak et al.
  • Journal Title: Plos One Volume: 8(9)
  • Peer Reviewed