| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Degradation of activated Src is regulated by Cdk5-dependent phosphorylation of Src Ser75. RGC loss was stimulated without elevated intraocular pressure in the aged phospho-mimicking mutant SrcSer75Asp mice (SD mice).To address the molecular mechanism of the degeneration induced by deregulation of the Ser75 phosphorylation, we established 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE) to globally detect changes in the retinal phosphoprotein abundance in retinas. The level of spot #30, which had an apparent molecular weight of 45kDa and an isoelectric point of 5.5～6.5, in SD mice was increased significantly than that of wild type mice.These results can lead to identification of retinal phosphoproteins changed by the Src-specific phosphorylation mutation, and are very important in elucidating the mechanism of RGC degeneration.
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