Role of immune networks of corneal endothelium and trabecular meshwork

• Project/Area Number: 25670734
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Tottori University
• Principal Investigator: Miyazaki Dai 鳥取大学, 医学部附属病院, 講師 (30346358)
• Co-Investigator(Kenkyū-buntansha): Inoue Yoshitsugu 鳥取大学医学部, 視覚病態学, 教授 (10213183)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 角膜内皮

Outline of Final Research Achievements

Corneal endotheliitis and anterior chamber inflammation cause bullous keratopathy or secondary glaucoma leading to significant visual morbidity. Clinical findings on etiology of diseases strongly suggested strong association with endothelial inflammation and intraocular pressure elevation (IOP). Upon inflammatory stimulus including viral infection, corneal endothelial cells provoke strong innate immune response as well as activation of acquired immune arm. Analysis of the mediators released by innate immune response showed significant association with clinical parameters of secondary glaucoma. Analysis of trabecular meshwork cells upon these stimuli suggested their roll on remodeling of trabecular meshwork.

Research Products

• [Journal Article] The effect of topical application of 0.15% ganciclovir gel on cytomegalovirus corneal endotheliitis. (2016)
  • Author(s): Koizumi N, Miyazaki D, Inoue T, Ohtani F, Kandori-Inoue M, Inatomi T, Sotozono C, Nakagawa H, Horikiri T, Ueta M, Nakamura T, Inoue Y, Ohashi Y, Kinoshita S.
  • Journal Title: Br J Ophthalmology Volume: May 3 Issue: 2 Pages: 114-119
  • DOI: 10.1136/bjophthalmol-2015-308238
  • Peer Reviewed / Open Access
• [Journal Article] Indoleamine 2,3-dioxygenase 1 in corneal endothelial cells limits herpes simplex virus type 1-induced acquired immune response (2015)
  • Author(s): Haruki T, Miyazaki D, Inata K, Sasaki S, Yamamoto Y, Kandori M, Yakura K, Noguchi Y, Touge C, Ishikura R, Touge H, Yamagami S, Inoue Y
  • Journal Title: Br J Ophthalmol. Volume: 99 Issue: 10 Pages: 1435-1442
  • DOI: 10.1136/bjophthalmol-2015-306863
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Clinical features and management of cytomegalovirus corneal endotheliitis: analysis of 106 cases from the Japan corneal endotheliitis study (2015)
  • Author(s): Koizumi N, Inatomi T, Suzuki T, Shiraishi A, Ohashi Y, Kandori M, Miyazaki D, Inoue Y, Soma T, Nishida K, Takase H, Sugita S, Mochizuki M, Kinoshita S; Japan Corneal Endotheliitis Study Group
  • Journal Title: Br J Ophthalmol Volume: 99 Issue: 1 Pages: 54-58
  • DOI: 10.1136/bjophthalmol-2013-304625
  • Peer Reviewed / Open Access
• [Journal Article] Relationship between the number of cytomegalovirus in anterior chamber and severity of anterior segment inflammation (2013)
  • Author(s): Kandori M, Miyazaki D, Yakura K, Komatsu N, Touge C, Ishikura R, Inoue Y
  • Journal Title: Jpn J Ophthalmol Volume: 57巻 Issue: 6 Pages: 497-502
  • DOI: 10.1007/s10384-013-0268-2
  • Peer Reviewed
• [Journal Article] Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. (2013)
  • Author(s): Miyazaki D1, Mihara S, Inata K, Sasaki S, Tominaga T, Yakura K, Ishida W, Fukushima A, Inoue Y.
  • Journal Title: Am J Pathol. Volume: 183 Issue: 1 Pages: 96-107
  • DOI: 10.1016/j.ajpath.2013.04.006
  • Peer Reviewed
• [Presentation] Activation of cytotoxic T lymphocytes by corneal endothelial cells after infection of human cytomegalovirus (2016)
  • Author(s): Ryu Uotani, Dai Miyazaki, Yoshitsugu Inoue, Mayumi Hosogai, Tatsuo Suzutani
  • Organizer: ARVO
  • Place of Presentation: Seattle, USA
  • Year and Date: 2016-05-01
  • Int'l Joint Research
• [Presentation] 血管新生緑内障の病態マーカーの探索 (2016)
  • Author(s): 宮崎大,蝶野郁世、小松直樹、武信二三枝、永瀬大輔、川本由紀美、春木智子、池田欣史、馬場高志、井上幸次
  • Organizer: 第120回日本眼科学会
  • Place of Presentation: 仙台
  • Year and Date: 2016-04-07
• [Presentation] Acquired immune response of corneal endothelial cell after cytomegalovirus infection (2016)
  • Author(s): Dai Miyazaki,Ryu Uotani, Michiko Kandori, Yoshitsugu Inoue, Satoru Yamagami, Tatsuo Suzutani, Mayumi Hosogai,Hiroki Isomura
  • Organizer: 第40回角膜カンファランス
  • Place of Presentation: 軽井沢
  • Year and Date: 2016-02-20
  • Int'l Joint Research
• [Presentation] Immune Privileged Role of Indoleamine 2,3-dioxygenase 1 in Corneal Endothelial Cells Exploited by Herpes Simplex Virus Type 1 (2015)
  • Author(s): Dai Miyazaki
  • Organizer: Asia ARVO
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2015-02-17
• [Presentation] Role of RAGE in interferon response of corneal endothelial cells after HSV-1 infection (2014)
  • Author(s): Dai Miyazaki, Michiko Kandori, Yumiko Noguchi, Keiko Yakura, Fumie Otani, Yoshitsugu Inoue, Satoru Yamagami
  • Organizer: International herpes virus workshop
  • Place of Presentation: 神戸ポートピアホテル（神戸市）
  • Year and Date: 2014-07-21
• [Presentation] HSV感染角膜内皮と自然免疫 (2014)
  • Author(s): 宮崎大
  • Organizer: 第51回眼感染症学会
  • Place of Presentation: 東京国際フォーラム（東京都千代田区）
  • Year and Date: 2014-07-05
  • Invited
• [Presentation] サイトメガロウイルスの角膜内皮感染応答 (2014)
  • Author(s): 宮崎大
  • Organizer: 第51回眼感染症学会
  • Place of Presentation: 東京国際フォーラム（東京都千代田区）
  • Year and Date: 2014-07-04
  • Invited
• [Presentation] サイトメガロウイルス感染に対する角膜内皮の分子応答
  • Author(s): 宮崎大
  • Organizer: 第２８回ヘルペスウイルス研究会
  • Place of Presentation: 淡路夢舞台国際会議場
• [Presentation] サイトメガロウイルス感染に対する角膜内皮の分子応答
  • Author(s): 宮崎大
  • Organizer: 第50回眼感染症学会
  • Place of Presentation: 大阪国際会議場
• [Presentation] 角膜内皮細胞の炎症応答におけるRAGE（終末糖化産物受容体）の役割
  • Author(s): 神鳥 美智子; 宮﨑 大; 野口 由美子; 矢倉 慶子; 大谷 史江;井上 幸次; 山上 聡
  • Organizer: 第３８回日本角膜学会総会
  • Place of Presentation: 沖縄コンベンションセンター