| Project/Area Number |
25670736
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Toda Munetoyo 京都府立医科大学, 医学(系)研究科(研究院), 助教(特任講座) (30550727)
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| Co-Investigator(Renkei-kenkyūsha) |
HAMURO JUNJI 京都府立医科大学, 医学系研究科, 教授 (80536095)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 角膜内皮細胞 / 再生医療 / miRNA / 細胞相転移 / 相転移 / エネルギー代謝 / 細胞表面マーカー |
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| Outline of Final Research Achievements |
We have defined the subpopulations (SPs) existing in the cultured human corneal endothelial cells (cHCECs) and identified the surface markers characteristic for each cHCEC SP, thereby have succeeded to establish the culture method to gain efficiently the high quality of cHCECs without cell state transition (CST). These SPs exhibited the distinctive proliferation propensity and features for energy metabolisms. The miRNA and the cytokines secreted to the culture supernatant (CS) were significantly different among these SPs. The CS of cHCECs without CST contained elevated levels of the selected species of miRNA, identical with those observed in CS of cHCECs of new born donors under one age old, indicating the relevance of miRNA interference present in infant HCECs.
All these findings altogether contribute to the elaboration to produce reproducibly the high quality of cHCECs without CST.
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