Research on pathogenesis of soft tissue arteriovenous malformations

• Project/Area Number: 25670753
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Kyorin University
• Principal Investigator: KURITA Masakazu 杏林大学, 医学部, 助教 (20424111)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 血管奇形 / 細胞培養 / 動静脈奇形 / 静脈奇形 / 血管内皮細胞 / 組織培養 / 血管新生 / 形成外科 / 初代培養

Outline of Final Research Achievements

In order to develop the new therapeutic intervention to soft tissue arteriovenous malformation, we develop the method of primary endothelial cell culture from small amount of vascular malformation resected samples. Using Rho-kinase inhibitor, Y27632, we established the method to obtain primary endothelial culture by enzymatic digestion and sorting.

Research Products

• [Presentation] 効率的な血管奇形組織からの血管内皮細胞単離法の開発 (2014)
  • Author(s): 佐藤卓士、栗田昌和、江藤ひとみ、菅浩隆、尾崎峰、井原玲、加地展之、多久嶋亮彦、波利井清紀
  • Organizer: 第23回日本形成外科学会基礎学術集会
  • Place of Presentation: キッセイ文化ホール
  • Year and Date: 2014-10-09 – 2014-10-10
• [Presentation] 効率的な血管奇形組織からの血管内皮細胞単離法の開発 (2014)
  • Author(s): 佐藤卓士、栗田昌和、江藤ひとみ、菅浩隆、尾崎峰、井原玲、加地展之、多久嶋亮彦、波利井清紀
  • Organizer: 第11回血管腫血管奇形研究会
  • Place of Presentation: 信州大学医学部附属病院外来棟4階大会議室
  • Year and Date: 2014-07-19 – 2014-07-20
• [Presentation] 動静脈奇形の臨床経験から学んだこと (2014)
  • Author(s): 栗田昌和、尾崎峰、井原玲、加地展之、多久嶋亮彦、波利井清紀
  • Organizer: 第57回日本形成外科学会総会・学術集会
  • Place of Presentation: 長崎ブリックホール
  • Year and Date: 2014-04-09 – 2014-04-11
• [Presentation] Eradication of extensive facial arteriovenous malformations with stepwise treatment composed of compartmentalization, percutaneous sclerotherapy, and surgical resection. (2014)
  • Author(s): Kurita M, Ozaki M, Sato D, Kaji N, Takushima A, Harii K
  • Organizer: The 20th ISSVA International Workshop
  • Place of Presentation: The 20th ISSVA International Workshop, Melbourne, Australia
  • Year and Date: 2014-04-02 – 2014-04-04
• [Presentation] 効率的な血管奇形組織からの血管内皮細胞単離法の開発 (2014)
  • Author(s): 佐藤卓士、栗田昌和、江藤ひとみ、菅浩隆、栗田恵里奈、尾崎峰、井原玲、多久嶋亮彦、波利井清紀
  • Organizer: 第25回東京大学医学部形成外科同門学術集会
  • Place of Presentation: 文京区、東京都