| Project/Area Number |
25670755
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKAZAWA Hiroaki 日本大学, 医学部, 教授 (60180270)
MATSUMOTO Taro 日本大学, 医学部, 教授 (50366580)
SOEJIMA Kazutaka 日本大学, 医学部, 准教授 (00246589)
SHIMODA Katsumi 日本大学, 医学部, 助教 (00266793)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Keywords | 脱分化脂肪細胞 / 血管新生作用 / 皮弁 / 同種 |
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| Outline of Final Research Achievements |
In recent years, pluripotent stromal cells such as mesenchymal stem cells (MSCs) and adipose-derived stem cells (ADSCs) have been developed, for use in the search for treatments of pathological conditions attributed to ischemia.By causing the dedifferentiation of porcine subcutaneous adipose tissue in vitro, we have established a cell culture method aimed at preparing dedifferentiated fat (DFAT) cells with a high proliferative capacity and pluripotency comparable to that of MSCs.
We have injected autologous and allogenic DFAT cells into random-pattern skin flaps in the back of rats, and examined the enlarging effect on the flap survival area. By injecting the cells into the base of the flap, we achieved a strong angiogenic effect and an enlarging effect on the flap survival area.
Because DFAT cells can easily be collected and cultured, a broad application in the treatment is expected in future, such as external injury and ischemic ulcer, in addition to the random pattern flap.
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