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Localization and regulation in reactive sites of DAMPs/PAMPs. Role of ATP from the activated plateles

Research Project

Project/Area Number 25670764
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Emergency medicine
Research InstitutionKagoshima University

Principal Investigator

MARUYAMA Ikuro  鹿児島大学, 医歯(薬)学総合研究科, 特任教授 (20082282)

Co-Investigator(Kenkyū-buntansha) ITO Takashi  鹿児島大学, 大学院医歯学総合研究科, 特任講師 (20381171)
Project Period (FY) 2013-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
KeywordsATP / DAMP / PAMP / platelet / inflammation / PAMPs / DAMPs / ATP / Adenosine / Inflammasome / IL-1β / IL-18
Research Abstract

Dual signalings from PAMPs/DAMPs play crucial role in inflammation, immunity and hemostasis. It has been listed more than 1000 of molecules as PAMPs, and DAMPs each other.
In this study, we took LPS as PAMPs,and HMGB-1 and ATP as DAMPs, and stimulated macrophages or dendritic cells. We showed that dual signaling from LPS and HMGB1 or ATP stimulated the release of IL-1b(beta) and IL-18.Thus dual signaling plays crucial role in inflammation, immunity and hemostasis. It has been described that platelet contains inflammasome, however the role of platelet-inflammasome has been not known. We examined the role of platelet inflammasome. An inflammasome inhibitor,1,5-anhydrofructose inhibited platelets aggregation with decreased release of IL-1b(beta). Thus ATP from activated platelets might play important role for the hemostasis/inflammation.

Report

(2 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (6 results)

All 2014 2013

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (2 results)

  • [Journal Article] Comparative evaluation of direct thrombin and factor Xa inhibitors with antiplatelet agents under flow and static conditions : an in vitro flow chamber model2014

    • Author(s)
      Hosokawa K, Ohnishi T, Sameshima H, Miura N, Koide T, Maruyama I, Tanaka KA
    • Journal Title

      PLoS One

      Volume: 9(1) Issue: 1 Pages: 86491-86491

    • DOI

      10.1371/journal.pone.0086491

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antithrombotic effects of PAR1 and PAR4 antagonists evaluated under flow and static conditions2014

    • Author(s)
      Hosokawa K, Ohnishi T, Miura N, Sameshima H, Koide T, Tanaka KA, Maruyama I
    • Journal Title

      Thromb Res

      Volume: 133(1) Issue: 1 Pages: 66-72

    • DOI

      10.1016/j.thromres.2013.10.037

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Recombinant thrombomodulin protects mice against histone-induced lethal thromboembolism.2013

    • Author(s)
      Nakahara M, Ito T, et al.
    • Journal Title

      PLoS One

      Volume: 8(9) Issue: 9 Pages: 75961-75961

    • DOI

      10.1371/journal.pone.0075961

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Saturated fatty acid palmitate induces extracellular release of histone H3 : a Possible mechanistic basis for high-fat diet-induced inflammation and thrombosis.2013

    • Author(s)
      Shrestha C, Ito T, et al.
    • Journal Title

      Biochemical and biophysical research communications

      Volume: 437(4) Issue: 4 Pages: 573-578

    • DOI

      10.1016/j.bbrc.2013.06.117

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Regulation of Danger Signals from HMGB1and Histones by Endothelial or Circulating Thrombomodulin2013

    • Author(s)
      Ikuro Maruyama
    • Organizer
      The Merinoff World Congress 2013 : HMGB1
    • Place of Presentation
      Manhasset, NY
    • Related Report
      2013 Final Research Report
  • [Presentation] Assay method of des-HMGB12013

    • Author(s)
      Ikuro Maruyama, Takashi Ito, Yoshio Azuma, Mayumi Nakahara, Teruto Hashiguchi, Shingo Yamada
    • Organizer
      N-terminus cleaved out HMGB1 by thrombin-thrombomodulin, and its clinical significance XXIVcongress of the ISTH
    • Place of Presentation
      Amsterdam, The Netherlands
    • Related Report
      2013 Final Research Report

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Published: 2014-07-25   Modified: 2016-11-25  

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