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Cellular conversion from non- skeletal to skeletal cells by using transactivation MyoD domain

Research Project

Project/Area Number 25670784
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Morphological basic dentistry
Research InstitutionTsurumi University

Principal Investigator

NIFUJI Akira  鶴見大学, 歯学部, 教授 (00240747)

Co-Investigator(Kenkyū-buntansha) ARAKI Ryoko  国立研究開発法人放射線医学総合研究所, 研究基盤センター, 室長 (40392211)
NAKASHIMA Kazuhisa  鶴見大学, 歯学部, 講師 (90252692)
IDENO Hisashi  鶴見大学, 歯学部, 学部助手 (40435699)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords遺伝子 / 分化 / 転写因子 / 骨格系組織 / 遺伝子発現 / 分化転換 / 転写活性 / MyoD
Outline of Final Research Achievements

Sets of specific transcription factors may induce cellular conversion from one committed cell to another. In the mesenchylmal cell lineages, the transcription factor MyoD can induce non-muscle cells to muscle cells. It is also reported that co-transfection of Sox9 with other sets of transcription factors may induce chondrocytes from non- chondrocytes. However reproducible and highly efficient cellular conversion from non- skeletal to skeletal cells is still challenging. In this study, we examined whether cellular conversion from non- skeletal to skeletal cells can be improved by using transactivation domain (TAD) of MyoD. Addition of TAD to Runx2 and sox9 enhanced activity of transcriptional transactivation. Cellular conversion rates by those factors did not change significantly, suggesting that additional factors or improvement of experimental condition are required for the cellular conversion of non- skeletal cells.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (26 results)

All 2015 2014 2013

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 4 results) Presentation (18 results)

  • [Journal Article] Coordinated expression of H3K9 histone methyltransferases during tooth development in mice2015

    • Author(s)
      Kamiunten T, Ideno H, Shimada A, Nakamura Y, Kimura H, Nakashima K, Nifuji A.
    • Journal Title

      Histochem Cell Biol.

      Volume: 143 Issue: 3 Pages: 259-266

    • DOI

      10.1007/s00418-014-1284-0

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Efficient expansion of mouse primary tenocytes using a novel collagen gel culture method2014

    • Author(s)
      Akemi Shimada, Satoshi Wada, Kouji Inoue, Hisashi Ideno, Taichi Kamiunten, Koichiro Komatsu, Akira Kudo, Yoshiki Nakamura,et al
    • Journal Title

      Histochem Cell Biol

      Volume: (in press) Issue: 2 Pages: 205-215

    • DOI

      10.1007/s00418-014-1191-4

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Search for conditions to detect epigenetic marks and nuclear proteins in immunostaining of the testis and cartilage2014

    • Author(s)
      Hisashi Ideno, Akemi Shimada, Taichi Kamiunten, Kazuhiko Imaizumi, Yoshiki Nakamura,et al
    • Journal Title

      Journal of Histology

      Volume: (in press) Pages: 1-8

    • DOI

      10.1155/2014/658293

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Promotion of Bone Formation around Alendronate-immobilized Screw-Type Titanium Implants after Implantation into Rat Molar Tooth Sockets2014

    • Author(s)
      Raita, Y, Komatsu, K, Nifuji, A, Sato, M, Morito, M, Hayakawa, T
    • Journal Title

      Journal of Hard Tissue Biology

      Volume: 23 Issue: 2 Pages: 177-186

    • DOI

      10.2485/jhtb.23.177

    • NAID

      130004480650

    • ISSN
      1341-7649, 1880-828X
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Predominant expression of H3K9 methyltransferases in prehypertrophic and hypertrophic chondrocytes during mouse growth plate cartilage development2013

    • Author(s)
      Ideno H, Shimada A, Imaizumi K, Kimura H, Abe M, Nakashima K, and Nifuji A
    • Journal Title

      Gene Expr Pat

      Volume: 13 Issue: 3-4 Pages: 84-90

    • DOI

      10.1016/j.gep.2013.01.002

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Alendronate promotes bone formation by inhibiting protein prenylation in osteoblasts in rat tooth replantation model2013

    • Author(s)
      Komatsu K, Shimada A, Shibata T, Wada S, Ideno H, Nakashima K, Amizuka N, Noda M, Nifuji A.
    • Journal Title

      Journal of Endocrinology

      Volume: 219 Issue: 2 Pages: 145-158

    • DOI

      10.1530/joe-13-0040

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Negligible immunogenicity of terminally differentiated cells derived from induced pluripotent or embryonic stem cells2013

    • Author(s)
      Ryoko Araki
    • Journal Title

      Nature

      Volume: 494 Issue: 7435 Pages: 100-104

    • DOI

      10.1038/nature11807

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms of skeletal tissue formation2013

    • Author(s)
      Nifuji A
    • Journal Title

      The Journal of Physical Fitness and Sports Medicine

      Volume: 2 Issue: 1 Pages: 1-8

    • DOI

      10.7600/jpfsm.2.1

    • NAID

      10031159011

    • ISSN
      2186-8123, 2186-8131
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Expressions and functions of Histone H3K9 methyltransferases during tooth development2014

    • Author(s)
      Taichi Kamiunten , Hisahi Ideno, Akemi Shimada, Satoshi Wada, Yoshiki Nakamura, Makoto Tachibana, Kazuhisa Nakashima, Akira Nifuji
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜( 神奈川県)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Annexin A5 null mice show masticatory muscle hypertrophy, hyper tooth attrition and bony outgrowth in the orofacial region2014

    • Author(s)
      Akemi Shimada 1, Yoshinori Arai 2, Koichiro Komatsu 1, Kazuhisa Nakashima 1, Teruhito Yamashita 3, Naoyuki Takahashi 3, Ernst Pöschl 4, Akira Nifuji 1
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜( 神奈川県)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Functions of histone methyltransferase G9a during growth and differentiation of mesenchymal cells2014

    • Author(s)
      Ideno H1,4, Shimada A1, Kamiunten T2, Wada S2, Komatsu K1, Tachibana M3, Araki R4, Nakamura Y2, Abe M4, Nakashima K1, Nifuji A1,4
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜( 神奈川県)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] The functions of histone methyltransferase G9a during growth and differentiation of mesenchymal cells2014

    • Author(s)
      出野 尚1,4, 島田明美1, 上運天太一2, 和田悟史2, 小松浩一郎1, 立花 誠3, 荒木良子4, 中村芳樹2, 安倍真澄4, 中島和久1,二藤 彰1,4
    • Organizer
      第56回 歯科基礎医学会
    • Place of Presentation
      福岡国際会議場( 福岡県)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Functions of the histone methyltransferase G9a during tenocyte differentiation2014

    • Author(s)
      和田悟史、出野 尚、島田明美、上運天太一、中島和久、中村芳樹、二藤 彰
    • Organizer
      第56回 歯科基礎医学会
    • Place of Presentation
      福岡国際会議場( 福岡県)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 歯の発生分化におけるヒストンメチル化酵素の発現と機能2014

    • Author(s)
      上運天太一、出野尚、島田明美、中島和久、中村芳樹、二藤 彰
    • Organizer
      第56回 歯科基礎医学会
    • Place of Presentation
      福岡国際会議場( 福岡県)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒストン3リジン9メチル化酵素群(G9a、Glp、Suv39h1、Prdm2)のマウ ス歯牙発生における発現と機能2014

    • Author(s)
      上運天太一、島田明美、出野尚、中村芳樹、中島和久、二藤彰
    • Organizer
      第32回 日本骨代謝学会
    • Place of Presentation
      大阪国際会議場( 大阪府)
    • Year and Date
      2014-07-24 – 2014-07-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 間葉系細胞の増殖・分化過程におけるヒストンメチル化酵素G9aの機能2014

    • Author(s)
      出野 尚1,2, 島田明美1, 上運天太一3, 和田悟史3, 小松浩一郎1, 中島和久1, 荒木良子2, 安倍真澄2, 二藤 彰1,2
    • Organizer
      第32回 日本骨代謝学会
    • Place of Presentation
      大阪国際会議場( 大阪府)
    • Year and Date
      2014-07-24 – 2014-07-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒストンメチル化酵素G9aによる腱細胞の分化制御2014

    • Author(s)
      和田悟史、島田明美、出野尚、中村芳樹、中島和久、二藤 彰
    • Organizer
      第32回 日本骨代謝学会
    • Place of Presentation
      大阪国際会議場( 大阪府)
    • Year and Date
      2014-07-24 – 2014-07-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 効率的で安定的なマウス腱細胞の初代培養法2014

    • Author(s)
      島田明美1, 和田悟史2, 出野尚1, 上運天太一2, 小松浩一郎1, 工藤明3, 中村芳樹2, 中島和久1, 二藤 彰1
    • Organizer
      第32回 日本骨代謝学会
    • Place of Presentation
      大阪国際会議場( 大阪府)
    • Year and Date
      2014-07-24 – 2014-07-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Predominant expression of Histone 3 lysine 9 methyltransferases in the prehypertrophc chondrocytes during the growth plate chondrocyte development2013

    • Author(s)
      Ideno H, Shimada A, Kamiunten T, Imaizumi, K, Nakashima K, Nifuji A
    • Organizer
      2nd Joint Meeting of the International Bone and Mineral Society and The Japanese Society for Bone and Mineral Research
    • Place of Presentation
      神戸国際会議場 兵庫県
    • Related Report
      2013 Research-status Report
  • [Presentation] Three histone 3 lysine 9 methyltransferases , G9a, GLP, and SETDB1, are expresssed during mouse tooth development.2013

    • Author(s)
      Kamiunten T, Shimada A, Ideno H, , Nakashima K, Nakamura Y, Nifuji A
    • Organizer
      2nd Joint Meeting of the International Bone and Mineral Society and The Japanese Society for Bone and Mineral Research
    • Place of Presentation
      神戸国際会議場 兵庫県
    • Related Report
      2013 Research-status Report
  • [Presentation] Tenocytes regulate cell survical of osteoblasts in vitro.2013

    • Author(s)
      Wada S, Shimada A, Ideno H, Nakamura Y, Nakashima K, Nifuji A
    • Organizer
      2nd Joint Meeting of the International Bone and Mineral Society and The Japanese Society for Bone and Mineral Research
    • Place of Presentation
      神戸国際会議場 兵庫県
    • Related Report
      2013 Research-status Report
  • [Presentation] Endocytic incorporation of alendronate promotes bone formation2013

    • Author(s)
      Komatsu K, Shimada A, Ideno H, Shibata T, Nakashima K, Amizuka N, Noda M, Nifuji A
    • Organizer
      2nd Joint Meeting of the International Bone and Mineral Society and The Japanese Society for Bone and Mineral Research
    • Place of Presentation
      神戸国際会議場 兵庫県
    • Related Report
      2013 Research-status Report
  • [Presentation] 発生の骨格組織分化におけるH3K9メチル化酵素群の発現局在2013

    • Author(s)
      二藤彰、 島田明美、 中島和久
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山コンベンションセンター 岡山県
    • Related Report
      2013 Research-status Report
  • [Presentation] MyoD mediated myogenic conversion from fibroblasts into myoblasts is blocked by cell cycle inhibition2013

    • Author(s)
      Hisashi Ideno, Kazuhisa Nakashima, Yasuyuki Ohkawa, Hiroshi Kimura , Ryoko Araki , Masumi Abe , Akira Nifuji
    • Organizer
      第36回 日本分子生物学会
    • Place of Presentation
      神戸国際会議場 兵庫県
    • Related Report
      2013 Research-status Report
  • [Presentation] Histone 3 lysine 9 methyltransferases , G9a, GLP, SUV39h1, and PRDM2, are expresssed during mouse tooth development.2013

    • Author(s)
      Kamiunten T, Ideno H, Shimada A, Nakamura Y, Nakashima K, Kimura H, Nifuji A
    • Organizer
      第36回 日本分子生物学会
    • Place of Presentation
      神戸国際会議場 兵庫県
    • Related Report
      2013 Research-status Report
  • [Presentation] Regulations of osteoblastic survival at entheses.2013

    • Author(s)
      Wada S, Shimada A, Ideno H, Nakamura Y, Nakashima K, Nifuji A
    • Organizer
      第36回 日本分子生物学会
    • Place of Presentation
      神戸国際会議場 兵庫県
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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