Communication between osteoblasts and osteoclasts in medaka

• Project/Area Number: 25670788
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional basic dentistry
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Kudo Akira 東京工業大学, 生命理工学研究科, 教授 (70178002)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 骨折修復 / 骨モデリング / メダカ / 骨芽細胞 / 破骨細胞 / TGFb / 尾ひれ / 咽頭歯 / OPG / 咽頭歯骨 / トランスジェニック / ノックアウトメダカ / RANKL / TGFbeta / ノックアウト

Outline of Final Research Achievements

To investigate TGFb function during fracture healing in vivo, we used the fracture healing model of the medaka fish, which enabled us to observe the behavior and function of living cells in response to a bone-specific injury. TGFb-2 functions specifically during fracture healing to stimulate the migration of osteoblasts as well as the differentiation of osteoblasts and osteoclasts. Next, we developed the bone modeling system by employing the pharyngeal bones, coupled with the osterix-DsRed/TRAP-GFP transgenic line visualizing osteoblasts and osteoclasts. Our results demonstrated that bone formation and resorption sites are divided by the posterior and anterior sides of developed supporting bones, respectively, and osteoblasts lead to coordination of bone morphology through the support to osteoclasts.

Research Products

• [Journal Article] Osteoblast and osteoclast behaviors in the turnover of attachment bones during medaka tooth replacement (2016)
  • Author(s): Mantoku A, Chatani M, Aono K, Inohaya K, Kudo A.
  • Journal Title: Developmental Biology Volume: 409 Issue: 2 Pages: 370-381
  • DOI: 10.1016/j.ydbio.2015.12.002
  • Peer Reviewed
• [Journal Article] TGFβ-2 signaling is essential for osteoblast migration and differentiation during fracture healing in medaka fish (2016)
  • Author(s): Takeyama K, Chatani M, Inohaya K, Kudo A.
  • Journal Title: Bone Volume: 86 Pages: 68-78
  • DOI: 10.1016/j.bone.2016.03.001
  • Peer Reviewed
• [Journal Article] Proliferation following tetraploidization regulates the size and number of erythrocytes in the blood flow during medaka development, as revealed by the abnormal karyotype of erythrocytes in the medaka TFDP1 mutant. (2015)
  • Author(s): Taimatsu K, Takubo K, Maruyama K, Suda T, Kudo A.
  • Journal Title: Dev Dyn. Volume: 該当なし Issue: 5 Pages: 651-668
  • DOI: 10.1002/dvdy.24259
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Microgravity promotes osteoclast activity in medaka fish reared at the international space station (2015)
  • Author(s): Masahiro Chatani, Akiko Mantoku, Kazuhiro Takeyama, Dawud Abduweri, Yasutaka Sugamori, Kazuhiro Aoki, Keiichi Ohya, Hiromi Suzuki, Satoko Uchida, Toru Sakimura, Yasushi Kono, Fumiaki Tanigaki, Masaki Shirakawa, Yoshiro Takano and *Akira Kudo
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 14172-14172
  • DOI: 10.1038/srep14172
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Eda/Edar signaling guides fin ray formation with preceding osteoblast differentiation, as revealed by analyses of the medaka all-fin less mutant afl (2014)
  • Author(s): Iida, Y., Hibiya, K., Inohaya, K. and Kudo, A
  • Journal Title: Deveopmental Dynamics Volume: 243 Pages: 765-777
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] In-vivo imaging of the fracture healing in medaka revealed two types of osteoclasts before and after the callus formation by osteoblasts (2014)
  • Author(s): Takeyama, K., Chatani, M., Takano, Y. and Kudo, A
  • Journal Title: Developmental Biology Volume: 394 Pages: 292-304
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Medaka Osteoclast in ISS. (2015)
  • Author(s): Akira Kudo
  • Organizer: 12th Japan-Korea joint seminar on space environment utilization
  • Place of Presentation: Yonsei University International Campus, Songdo, Incheon, South Korea
  • Year and Date: 2015-10-20
  • Int'l Joint Research / Invited
• [Presentation] RANKL/OPG double deficient medaka unveils the decision system for the bone resorption site in a whole-body. (2015)
  • Author(s): Masahiro Chatani, Yoshiro Takano, Takeshi Todo, Akira Kudo
  • Organizer: the ASBMR 2015 Annual Meeting
  • Place of Presentation: Seattle, WA, USA
  • Year and Date: 2015-10-09
  • Int'l Joint Research
• [Presentation] Early response to microgravity in the analysis of whole transcriptome and live imaging. (2015)
  • Author(s): Masahiro Chatani, Akiko Mantoku, Kazuhiro Takeyama, Hiroya Morimoto, Takehiko Ito, Naoki Tanigawa, Koji Kubota, Hiromi Suzuki, Satoko Uchida, Japan; Fumiaki Tanigaki, Masaki Shirakawa, Yoshiro Takano, Akira Kudo
  • Organizer: the ASBMR 2015 Annual Meeting
  • Place of Presentation: Seattle, WA, USA
  • Year and Date: 2015-10-09
  • Int'l Joint Research
• [Presentation] Microgravity enhances osteoclast activity revealed by 2 months-rearing of medaka fish in ISS (2015)
  • Author(s): Masahiro Chatani, Akiko Mantoku, Kazuhiro Takeyama, Dawud Abduweri, Kazuhiro Aoki, Yasutaka Sugamori, Keiichi Ohya, Satoko Uchida, Hiromi Suzuki, Toru Sakimura, Yasushi Kono, Fumiaki Tanigaki, Masaki Shirakawa, Yoshiro Takano and Akira Kudo
  • Organizer: ISSR&D Conference 2015
  • Place of Presentation: Marriot Copley Place, Boston, MA, USA
  • Year and Date: 2015-07-07
  • Int'l Joint Research
• [Presentation] TGFbeta signaling activated by MMP2 is essential for bone fracture healing (2015)
  • Author(s): Kazuhiro Takeyama, Masahiro Chatani, Akira Kudo
  • Organizer: 9th European Zebrafish Meeting
  • Place of Presentation: Oslo, Norway
  • Year and Date: 2015-06-28
  • Int'l Joint Research
• [Presentation] The whole-body analysis employing RANKL -/- and OPG -/- medaka fish reveals the in vivo bone resorption system (2014)
  • Author(s): Masahiro Chatani, Yoshiro Takano, Takeshi Todo, Akira Kudo
  • Organizer: Annual Meeting of ASBMR (The American Society for Bone and Mineral Research) 2014
  • Place of Presentation: Houston, Texas, USA
  • Year and Date: 2014-09-12 – 2014-09-15
• [Presentation] TGFbによる骨折修復初期の破骨細胞と骨芽細胞の誘導 (2014)
  • Author(s): 武山和弘、茶谷昌宏、高野吉郎、工藤 明
  • Organizer: 第32回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場、大阪市
  • Year and Date: 2014-07-24 – 2014-07-26
• [Presentation] メダカを用いたRANKL/OPGのバランス破壊による新たな全身骨吸収機構の解明 (2014)
  • Author(s): 茶谷昌宏、高野吉郎、藤堂 剛、工藤 明
  • Organizer: 第32回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場、大阪市
  • Year and Date: 2014-07-24 – 2014-07-26
• [Presentation] Tooth regeneration with the cooperative action of osteoclasts and osteoblasts (2014)
  • Author(s): Akiko Mantoku, Masahiro, Chatani, Keiji Inohaya, Akira Kudo
  • Organizer: 47 th Annual Meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: WINC AICHI, 名古屋市
  • Year and Date: 2014-05-27 – 2014-05-30
• [Presentation] メダカを用いたRANKL/OPGのバランス破壊による新たな全身骨吸収機構の解明 (2014)
  • Author(s): 茶谷昌宏
  • Organizer: 日本骨代謝学会
  • Place of Presentation: 大阪国際会議場
• [Presentation] Tooth rgeneration with the ccoperative action of osteoclasts and osteoblasts
  • Author(s): Akiko Mantoku
  • Organizer: 日本発生生物学会
  • Place of Presentation: ウィンクあいち