| Project/Area Number |
25670788
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional basic dentistry
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Kudo Akira 東京工業大学, 生命理工学研究科, 教授 (70178002)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 骨折修復 / 骨モデリング / メダカ / 骨芽細胞 / 破骨細胞 / TGFb / 尾ひれ / 咽頭歯 / OPG / 咽頭歯骨 / トランスジェニック / ノックアウトメダカ / RANKL / TGFbeta / ノックアウト |
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| Outline of Final Research Achievements |
To investigate TGFb function during fracture healing in vivo, we used the fracture healing model of the medaka fish, which enabled us to observe the behavior and function of living cells in response to a bone-specific injury. TGFb-2 functions specifically during fracture healing to stimulate the migration of osteoblasts as well as the differentiation of osteoblasts and osteoclasts. Next, we developed the bone modeling system by employing the pharyngeal bones, coupled with the osterix-DsRed/TRAP-GFP transgenic line visualizing osteoblasts and osteoclasts. Our results demonstrated that bone formation and resorption sites are divided by the posterior and anterior sides of developed supporting bones, respectively, and osteoblasts lead to coordination of bone morphology through the support to osteoclasts.
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