Exploration of seeds for development of a new drug targeting Bone-Vascular-Spleen axis
Project/Area Number |
25670793
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Functional basic dentistry
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Research Institution | Matsumoto Dental University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
NINOMIYA Tadashi 松本歯科大学, 総合歯科医学研究所, 講師 (00360222)
NAKAMICHI Yuko 松本歯科大学, 総合歯科医学研究所, 講師 (20350829)
NAKAMURA Midori 松本歯科大学, 歯学部, 准教授 (90278177)
UEHARA Syunsuke 松本歯科大学, 歯学部, 講師 (90434480)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 破骨細胞 / 骨芽細胞 / マクロファージ / 脾臓 / RANKL / M-CSF / IL-34 / ミクログリア / 皮膚 / 脳 / ビタミンD / 単球 / 血管 |
Outline of Final Research Achievements |
(1)To clarify the role of spleen in bone metabolism, M-CSF-deficient mice and wild-type mice were subjected to splenectomy or a sham-operation. Splenectomy completely suppressed osteoclastogenesis in M-CSF-deficient mice. In contrast, splenectomy moderately suppressed osteoclastogenesis in wild-type mice. (2)Comparing the gene expression profiles of IL-34-positive and IL-34-negative vascular endothelial cells identifies differentially expressed genes. We focused on top-ranked genes which possess leading signal peptide for secretion and membrane-anchoring.We are now addressing functional analyses of these genes. (3)To elucidate mechanisms underlying the age-associated increase of IL-34 expressions in several tissues, we tried to establish the culture system for IL-34-positive vascular endothelial cells. However, the expression level of IL-34 gradually decreased in long-term culture.
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Report
(3 results)
Research Products
(10 results)
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[Journal Article] The dynamin inhibitor dynasore inhibits bone resorption by rapidly disrupting actin rings of osteoclasts.2015
Author(s)
Thirkonda, G.J., Uehara, S., Nakayama, T., Yamashita, T., Nakamura, Y., Mizoguchi, T., Yagami, K., Takahashi, N., Udagawa, N., Kobayashi, Y.
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Journal Title
J. Bone Miner. Metab.
Volume: 33
Related Report
Peer Reviewed
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[Journal Article] Noncanonical Wnt5a enhances Wnt/-catenin signaling during osteogenesis.2014
Author(s)
Okamoto, M., Udagawa, N., Yamashita, T., Nakamichi, Y., Uehara, S., Kato, H., Saito, N., Minami, Y., Takahashi, N., Kobayashi, Y.
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Journal Title
Sci. report
Volume: 4
Issue: 1
Pages: 4493-4493
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Stimulation of bone formation in cortical bone of mice treated with a receptor activator of nuclear factor-κB ligand (RANKL)-binding peptide that possesses osteoclastogenesis inhibitory activity.2013
Author(s)
Furuya Y, Inagaki A, Khan M, Mori K, Penninger JM, Nakamura M, Udagawa N, Aoki K, Ohya K, Uchida K, Yasuda H
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Journal Title
J Biol Chem
Volume: 288
Issue: 8
Pages: 5562-5571
DOI
Related Report
Peer Reviewed
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[Book] Encyclopedia of Human Body 3rd Edition2015
Author(s)
Takahashi, N., Mizoguchi, T., Nakamichi, Y., Kobayashi, Y., Nakamura, M., Hofstetter, W., Udagawa, N.
Related Report