Identification of stromal cell-derived factors required for maintaining functional osteoclast precursors and elucidation of the role of the factors in inflammatory bone destruction
| Project/Area Number |
25670799
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Meikai University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 破骨細胞前駆細胞株4B12細胞 / 破骨細胞前駆細胞機能維持因子 / Insulin / Fibronectin N末端 / IGFBP-2 / Nidogen-2 / CD13 / 破骨細胞形成 / 破骨細胞前駆細胞株4Ｂ12細胞 / 破骨細胞前駆細胞分化機能維持因子 / Fibronectin |
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| Outline of Final Research Achievements |
M-CSF, Insulin-2, Nidogen-2, IGFBP-2 and N-terminal fragment 30-kDa of fibronectin (Fn30kDa) were identified as factors required for maintenance and proliferation of osteoclast precursor in conditioned media from mouse embryonic calvaria-derived stromal cells. The amino acid sequence NGRGE were conserved between IGFBP2 and Fn30kDa. Fn30kDa bound to CD13 expressed on osteoclast precursors, increased the expression of RANK, TRAF6, NFATc1 and c-Fos, and enhanced osteoclastogenesis. Intraperitoneal injection of Fn30kDa in mice increased the number of CD11b/CD13 positive cells in peripheral blood, and enhanced calvarial bone resorption induced by LPS.
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Report
(4 results)
Research Products
(5 results)
