The individual difference in the dentin deterioration by Genetic Analysis

• Project/Area Number: 25670804
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Hokkaido University
• Principal Investigator: Sano Hidehiko 北海道大学, 歯学研究科(研究院), 教授 (90205998)
• Co-Investigator(Kenkyū-buntansha): MATSUDA Yasuhiro 北海道医療大学, 歯学部口腔機能修復・再建学系・う蝕制御治療学分野, 講師 (50431317) ; 中沖 靖子 東京医科歯科大学, 歯学部, その他 (50302881) ; 川本 千春 北海道大学, 大学病院, 助教 (60301909)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: High Resolution Melt分析法 / ＤＮＡ抽出 / 個体差 / DNA / SNP / 象牙質 / 象牙質劣化 / ｐHサイクル / 遺伝子解析 / 象牙室 / 微小引っ張り強さ試験

Outline of Final Research Achievements

The dNA extraction procedure from pulp and the dentin specimens of tooth was able to get DNA of 9.8ng/μl and 5.8 ng/μl. In addition, High Resolution Melt analytical method (HRMA) analysis was performed for rs17576 SNP in MMP9. The frequency of the genetic polymorphism was also analyzed based on th Database of single nucleotide polymorphisms (SNPs) at the Naciotnal Center for Biotechnology Information. The frequency of A/A, A/G, G/G in the rs17576 SNP is 36.7% 44,6%, 18,7% . It was classified in three groups in the analysis by HRMA on the basis of data of NCBI equally by the data of 10004 samples. A method to analyze was established by dentin deterioration by genetic polymorphism by combining it with the load examination using the device for an automatic pH cycle.

Research Products

• [Journal Article] Evaluation of microtensile bond strength of self-etching adhesives on normal and caries-affected dentin (2016)
  • Author(s): Shibata S, Vieira L. C, Baratieri L. N, Fu J, Hoshika S, Matsuda Y, Sano H.
  • Journal Title: Dental Materials Journal Volume: 35 Issue: 2 Pages: 166-173
  • DOI: 10.4012/dmj.2014-330
  • NAID: 130005141811
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed
• [Journal Article] Assessment of the inhibitory effects of fissure sealants on the demineralization of primary teeth using an automatic pH-cycling system (2016)
  • Author(s): Ushimura, S, Nakamura K, Matsuda Y, Minamikawa H, Abe S, Yawaka Y.
  • Journal Title: Dental Materials Journal Volume: 35 Issue: 2 Pages: 316-324
  • DOI: 10.4012/dmj.2015-297
  • NAID: 130005141794
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed
• [Journal Article] う蝕リスクの遺伝子変異 (2015)
  • Author(s): 松田康裕
  • Journal Title: 北海道歯学雑誌 Volume: 36（1） Pages: 21-23
  • NAID: 120005676289
• [Journal Article] Fluorine uptake into the human enamel surface from fluoride-containing sealing materials during cariogenic pH cycling (2015)
  • Author(s): Matsuda Yasuhiro, Okuyama Katsushi, Yamamoto Hiroko, Komatsu Hisanori, Koka Masashi, Sato Takahiro, Hashimoto Naoki, Oki Saiko, Kawamoto Chiharu, Sano Hidehiko
  • Journal Title: Nuclear Instruments and Methods in Physics Research B Volume: 348 Pages: 156-159
  • DOI: 10.1016/j.nimb.2015.01.062
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of caries progression in dentin treated by fluoride-containing materials using an in-air micro-PIGE and micro-PIXE measurement system (2015)
  • Author(s): H. Yamamoto, Y. Iwami, K. Yagi, M. Hayashi, H. Komatsu, K. Okuyama, Y. Matsuda, K.Yasuda
  • Journal Title: Nuclear Instruments & Methods in Physics Research Volume: B348 Pages: 152-155
  • DOI: 10.1016/j.nimb.2015.01.068
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A new technique for analyzing trace element uptake by human enamel (2015)
  • Author(s): Funato Y, Matsuda Y, Okuyama K, Yamamoto H, Komatsu H, Sano H
  • Journal Title: Dental Materials Journal Volume: 34 Issue: 2 Pages: 240-245
  • DOI: 10.4012/dmj.2014-127
  • NAID: 130005062410
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effect of different remaining dentin thickness on microtensile bond strength of current adhesive systems (2015)
  • Author(s): Ting S, Chowdhury AFM A, Pan F, Fu J, Sun J, Kakuda S, Hoshika S, Matsuda Y, Ikeda T, Nakaoki Y, Abe S, Yoshida Y, Sano H
  • Journal Title: Dental Materials Journal Volume: 34（2） Pages: 181-188
  • Peer Reviewed
• [Presentation] Evaluation of dentin-bound fluoride uptaken from fluoride-containing dental materials. (2016)
  • Author(s): Okuyama K, Matsuda Y, Yamamoto H, Komada Y, Abe M, Sano H, Tamaki Y
  • Organizer: 45th Meeting of AADR (American association for dental research)
  • Place of Presentation: Los Angeles, U.S.A.
  • Year and Date: 2016-03-16
  • Int'l Joint Research
• [Presentation] Caries prevention after surface reaction-type prereacted glass ionomer filler-containing coating resin removal from root surfaces. (2015)
  • Author(s): Miyata Saori, Tanaka Saori, Matsuda Yasuhiro, Hashimoto Naoki, Sano Hidehiko and Kawakami Masamitsu
  • Organizer: Nano Korea 2015 Symposium
  • Place of Presentation: Seul, Korea
  • Year and Date: 2015-07-01
  • Int'l Joint Research
• [Presentation] Quantitative analysis of fluorine uptake in pre-demineralized enamel from filling materials (2015)
  • Author(s): Yasuhiro, Hashimoto Naoki, Okuyama Katsushi, Koka Masashi, Sato Takahiro, Nakamura Koichi, Abe Shigeaki and Sano Hidehiko
  • Organizer: Nano Korea 2015 Symposium
  • Place of Presentation: Seul, Korea
  • Year and Date: 2015-07-01
  • Int'l Joint Research
• [Presentation] 24.Fluorine uptake into human enamel surface from fluoride-containing sealing materials during cariogenic pH cycling. (2014)
  • Author(s): Matsuda Y. Okuyama K. Yamamoto H. Koka M. Sato T. Komatsu,H. Sano,H.
  • Organizer: 14th International Conference on Nuclear Microprobe Technology and Applications
  • Place of Presentation: Padva, Italy
  • Year and Date: 2014-07-06 – 2014-07-11