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Development of a new strategy for regeneration of alveolar bone by combination of dental stem cells and the functional scaffold

Research Project

Project/Area Number 25670806
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Conservative dentistry
Research InstitutionTohoku University

Principal Investigator

SHIMAUCHI Hidetoshi  東北大学, 歯学研究科(研究院), 教授 (70187425)

Co-Investigator(Kenkyū-buntansha) ISHIHATA Hiroshi  東北大学, 大学院歯学研究科, 助教 (40261523)
NEMOTO Eiji  東北大学, 大学院歯学研究科, 准教授 (40292221)
KANYA Sousuke  東北大学, 大学院歯学研究科, 助教 (80375097)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords歯槽骨再生 / 幹細胞 / 新規スキャフォールド / 歯髄幹細胞 / チタンメッシュ / 歯周組織再生 / 機能的スキャフォールド / ナノ化ハイドロキシアパタイト
Outline of Final Research Achievements

This research project was aimed to develop the new strategy of alveolar bone regeneration by a combination of dental stem cells and guided bone regeneration technique using a newly developed titanium (Ti) mesh membrane. Our in vitro studies revealed the molecular mechanisms of proliferation and differentiation of dental stem cells regulated by Wnt signaling. Dental folllicule cells (DFC) are able to differentiate into cement blast and alveolar bone cells. Canonical and non-canonical Wnt signaling performed the complementary role on DFC, that canonical Wnt3a signaling induced the osteoblastogenesis of DFC and non-canonical Wnt5a signaling negative developed these processes.
Our newly developed Ti-mesh exhibited the proliferative effects on DFCs by a topographical effect. Furthermore, our animal studies indicated that a combination of graft and Ti-mesh induced the superior bone regeneration on standardized bone defect model made in beagle dogs.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (8 results)

All 2015 2014 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (6 results)

  • [Journal Article] Calcium Phosphate Particles Induce Interleukin-8 Expression in Human Gingival Epithelial Cell Line via Nuclear Factor-kB Signaling Pathway2014

    • Author(s)
      Sakai, Y., E. Nemoto, S. Kanaya, M. Shimonishi, H. Shimauchi.
    • Journal Title

      J. Periodontol.

      Volume: 85 Pages: 464-1473

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cyclic tensile force up-regulates BMP-2 expression through MAP kinase and COX-2/PGE2 signaling pathways in human periodontal ligament cells2014

    • Author(s)
      Suzuki, R., E. Nemoto, H. Shimauchi.
    • Journal Title

      Exp. Cell Res.

      Volume: 323 Pages: 232-241

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] 歯小嚢細胞におけるWnt/β-cateninシグナルによるOsterix発現誘導へのp38MAPキナーゼの関与2015

    • Author(s)
      向阪 幸彦、根本 英二、金谷 聡介、中村 卓史、田村 正人、島内 英俊
    • Organizer
      第58回春季日本歯周病学会学術大会
    • Place of Presentation
      千葉市,千葉
    • Year and Date
      2015-05-15 – 2015-05-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Wnt5a Attenuates Dental Follicle Cells Differentiation Triggered by Wnt3a2015

    • Author(s)
      Sakisaka Y., Nemoto E., Suto M., Kanaya S., Tamura M., Shimauchi H.
    • Organizer
      The 93rd International Association for Dental Research General Session & Exhibition
    • Place of Presentation
      Boston, MA, USA
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] Non-Canonical Wntがセメント芽細胞の分化に与える影響2014

    • Author(s)
      向阪 幸彦、根本 英二、須藤 瑞樹、金谷 聡介、田村 正人、島内 英俊
    • Organizer
      第140回日本歯科保存学会春季学術大会
    • Place of Presentation
      大津市,滋賀
    • Year and Date
      2014-06-19 – 2014-06-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] Wnt/β-cateninシグナルによる歯小嚢細胞アルカリフォスファターゼの発現誘導2014

    • Author(s)
      向阪 幸彦、根本 英二、須藤 瑞樹、金谷 聡介、田村 正人、島内 英俊
    • Organizer
      第57回春季日本歯周病学会学術大会
    • Place of Presentation
      岐阜市,岐阜
    • Year and Date
      2014-05-23 – 2014-05-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Wnt/β-cateninシグナルによる歯小嚢細胞アルカリフォスファターゼの発現誘導2014

    • Author(s)
      向阪幸彦、根本英二、金谷聡介、田村正人、島内英俊
    • Organizer
      第57回春季日本歯周病学会学術大会
    • Place of Presentation
      岐阜市
    • Related Report
      2013 Research-status Report
  • [Presentation] Induction of BMP-2 by extracellular Ca2+ and Pi as well as nano-hydroxyapatite in periodontal ligament cells.2013

    • Author(s)
      Suto M, Nemoto E, Kanaya S, Suzuki R, Tsuchiya M, Shimauchi H.
    • Organizer
      10th Asian Pacific Society of Periodontology Meeting
    • Place of Presentation
      奈良市
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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