| Project/Area Number |
25670806
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Conservative dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ISHIHATA Hiroshi 東北大学, 大学院歯学研究科, 助教 (40261523)
NEMOTO Eiji 東北大学, 大学院歯学研究科, 准教授 (40292221)
KANYA Sousuke 東北大学, 大学院歯学研究科, 助教 (80375097)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 歯槽骨再生 / 幹細胞 / 新規スキャフォールド / 歯髄幹細胞 / チタンメッシュ / 歯周組織再生 / 機能的スキャフォールド / ナノ化ハイドロキシアパタイト |
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| Outline of Final Research Achievements |
This research project was aimed to develop the new strategy of alveolar bone regeneration by a combination of dental stem cells and guided bone regeneration technique using a newly developed titanium (Ti) mesh membrane. Our in vitro studies revealed the molecular mechanisms of proliferation and differentiation of dental stem cells regulated by Wnt signaling. Dental folllicule cells (DFC) are able to differentiate into cement blast and alveolar bone cells. Canonical and non-canonical Wnt signaling performed the complementary role on DFC, that canonical Wnt3a signaling induced the osteoblastogenesis of DFC and non-canonical Wnt5a signaling negative developed these processes.
Our newly developed Ti-mesh exhibited the proliferative effects on DFCs by a topographical effect. Furthermore, our animal studies indicated that a combination of graft and Ti-mesh induced the superior bone regeneration on standardized bone defect model made in beagle dogs.
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