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Application of activated macrophages derived from dental pulp stem cells to dental pulp tissue engineering

Research Project

Project/Area Number 25670808
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Conservative dentistry
Research InstitutionNiigata University

Principal Investigator

OKIJI Takashi  新潟大学, 医歯学系, 教授 (80204098)

Co-Investigator(Kenkyū-buntansha) YOSHIBA Nagako  新潟大学, 医歯学総合病院, 講師 (10323974)
YOSHIBA Kunihiko  新潟大学, 医歯学系, 准教授 (30220718)
KANEKO Tomoatsu  新潟大学, 医歯学総合病院, 助教 (70345297)
SHIGETANI Yoshimi  新潟大学, 医歯学総合病院, 助教 (80397132)
OHKURA Naoto  新潟大学, 医歯学総合病院, 医員 (00547573)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords歯内治療学 / 歯髄組織再生 / 歯髄幹細胞 / マクロファージ / スキャホールド
Outline of Final Research Achievements

This study aimed to examine the role of macrophages in the regeneration of dental pulp tissues. After co-implantation of ED2-positive macrophages isolated from rat dental pulp with stem cells into the coronal pulp chamber of pulpotomized rat molars, expression of macrophage markers in the engineered pulp tissue was analyzed. When IL-4 treated ED2-positive macrophages were co-implanted with stem cells, the engineered tissues showed a significant increase in the number of ED2-positive cells and the expression of CD34 and CD163 mRNAs. These findings suggest that M2 macrophages show proliferation and act as wound healing macrophages after co-implantation of macrophages with stem cells.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (12 results)

All 2015 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (8 results) (of which Invited: 1 results) Book (1 results)

  • [Journal Article] 幹細胞を用いた歯髄組織再生におけるマクロファージ様細胞について2015

    • Author(s)
      金子友厚
    • Journal Title

      日本歯内療法学会雑誌

      Volume: 36 Pages: 13-16

    • NAID

      130006108659

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Initial transient accumulation of M2 macrophage-associated molecule-expressing cells after pulpotomy with mineral trioxide aggregate in rat molars2014

    • Author(s)
      Takei E, Shigetani Y, Yoshiba K, Hinata G, Yoshiba N, Okiji T
    • Journal Title

      Journal of Endodontics

      Volume: 40 Issue: 12 Pages: 1983-1988

    • DOI

      10.1016/j.joen.2014.08.012

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] M2 macrophages participate in the biological tissue healing reaction to mineral trioxide aggregate2014

    • Author(s)
      Ito T, Kaneko T, Yamanaka Y, Shigetani Y, Yoshiba K, Okiji T
    • Journal Title

      Journal of Endodontics

      Volume: 40 Issue: 3 Pages: 379-383

    • DOI

      10.1016/j.joen.2013.11.011

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Immnue-LCM analysis of M1/M2 macrophages in engineered dental pulp tissues2014

    • Author(s)
      T. Kaneko
    • Organizer
      18th International Microscopy Congress
    • Place of Presentation
      Prague Congress Centre (Prague, チェコ共和国)
    • Year and Date
      2014-09-07 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 再生歯髄組織におけるマクロファージの活性化と成熟 ―血管内皮細胞混合移植の影響―2014

    • Author(s)
      金子友厚
    • Organizer
      第35回日本歯内療法学会学術大会
    • Place of Presentation
      朱鷺メッセ(新潟市)
    • Year and Date
      2014-07-12 – 2014-07-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] ラット実験的歯髄炎における幹細胞関連マーカー発現の経時的検索2014

    • Author(s)
      伊藤崇史
    • Organizer
      日本歯科保存学会2014年度春季学会(第140回)
    • Place of Presentation
      びわ湖ホール(大津市)
    • Year and Date
      2014-06-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] 再生歯髄組織内マクロファージにおけるM1/M2関連遺伝子の発現2014

    • Author(s)
      金子友厚
    • Organizer
      日本歯科保存学会2014年度春季学会(第140回)
    • Place of Presentation
      びわ湖ホール(大津市)
    • Year and Date
      2014-06-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] Vital Pulp Therapy: Biological Basis, Current Concepts and Future Perspectives2013

    • Author(s)
      Okiji T
    • Organizer
      The 9th World Endodontic Congress
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Immunohistochemical analysis of CD163-expressing macrophages in mineral trioxide aggregate-applied rat subcutaneous tissue2013

    • Author(s)
      Ito T, Yamanaka Y, Kaneko T, Okiji T
    • Organizer
      The 9th World Endodontic Congress
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] The activation/maturation of macrophages in engineered pulp tissue2013

    • Author(s)
      Kaneko T, Yamanaka Y, Ito T, Yoshiba K, Okiji T
    • Organizer
      The 9th World Endodontic Congress
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] Accumulation of M2 macrophages in mineral trioxide aggregate-implanted tissue2013

    • Author(s)
      Ito T, Kaneko T, Yamanaka Y, Okiji T
    • Organizer
      15th Joint Scientific Meeting of JSCD-KACD
    • Place of Presentation
      Gyeongju, Korea
    • Related Report
      2013 Research-status Report
  • [Book] ライフステージと歯内療法2013

    • Author(s)
      金子友厚, 興地隆史(分担執筆)
    • Total Pages
      147
    • Publisher
      デンタルダイヤモンド社
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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