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The novel strategy to regenerate periodontal ligament tissue using iPS cells

Research Project

Project/Area Number 25670811
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Conservative dentistry
Research InstitutionKyushu University

Principal Investigator

AKAMINE Akifumi  九州大学, 歯学研究科(研究院), 教授 (00117053)

Co-Investigator(Kenkyū-buntansha) WADA Naohisa  九州大学, 大学病院, 講師 (60380466)
MAEDA Hidefumi  九州大学, 大学病院, 講師 (10284514)
MONNOUCHI Satoshi  九州大学, 歯学研究院, 助教 (30609558)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords歯根膜組織 / iPS細胞 / ハイブリッドスキャフォールド / 再生
Outline of Final Research Achievements

In this study, we aimed to establish novel method to regenerate periodontal tissue by use of iPS cells. We examined the effects of new cell differentiation method and scaffold where cells attach and form tissue. When human periodontal ligament cells (HPDLCs) were cultured on the hybrid scaffolds, HPDLCs attached and proliferated well on the scaffold, and also formed extra cellular matrix tissue. In addition, co-culture of neural crest cells differentiated from iPS cells with HPDLCs in the presence of TGF-beta upregulated the expression of periodontal ligament-related markers, suggesting iPS cell differentiation into HPDLC-like cells. Although further studies are required, the combination of iPS cells induced to differentiate into neural crest-like cells and hybrid scaffold could be alternative cell source for novel cell transplantation method to regenerate periodontal ligament tissue.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 2 results) Presentation (1 results) (of which Invited: 1 results)

  • [Journal Article] Wnt5a induces collagen production by human periodontal ligament cells through TGFβ1-mediated upregulation of Periostin expression2015

    • Author(s)
      Hasegawa D, Wada N, Maeda H, Yoshida S, Mitarai H, Tomokiyo A, Monnouchi S, Hamano S, Yuda A, Akamine A
    • Journal Title

      J Cell Physiol.

      Volume: Epub ahead of print Issue: 11 Pages: 2647-2660

    • DOI

      10.1002/jcp.24950

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Semaphorin 3A induces mesenchymal stem-like properties in human periodontal ligament cells2014

    • Author(s)
      Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Yoshida S, Tomokiyo A, Monnouchi S, Akamine A
    • Journal Title

      Stem Cells Dev

      Volume: (Epub ahead of print) Issue: 18 Pages: 2225-2236

    • DOI

      10.1089/scd.2013.0405

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regeneration of the periodontium for preservation of the damaged tooth2014

    • Author(s)
      Maeda H, Tomokiyo A, Wada N, Koori K, Kawachi G, Akamine A.
    • Journal Title

      Histol Histopathol.

      Volume: 29 Pages: 1249-1262

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 歯根膜幹細胞の多様な特性およびその有用性2014

    • Author(s)
      和田尚久
    • Organizer
      第56回歯科基礎医学会学術大会 サテライトシンポジウム「間葉系幹細胞の直接的/間接的な組織再生への関与を考える」
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2014-09-25
    • Related Report
      2014 Annual Research Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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