The novel strategy to regenerate periodontal ligament tissue using iPS cells

• Project/Area Number: 25670811
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Kyushu University
• Principal Investigator: AKAMINE Akifumi 九州大学, 歯学研究科(研究院), 教授 (00117053)
• Co-Investigator(Kenkyū-buntansha): WADA Naohisa 九州大学, 大学病院, 講師 (60380466) ; MAEDA Hidefumi 九州大学, 大学病院, 講師 (10284514) ; MONNOUCHI Satoshi 九州大学, 歯学研究院, 助教 (30609558)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 歯根膜組織 / iPS細胞 / ハイブリッドスキャフォールド / 再生

Outline of Final Research Achievements

In this study, we aimed to establish novel method to regenerate periodontal tissue by use of iPS cells. We examined the effects of new cell differentiation method and scaffold where cells attach and form tissue. When human periodontal ligament cells (HPDLCs) were cultured on the hybrid scaffolds, HPDLCs attached and proliferated well on the scaffold, and also formed extra cellular matrix tissue. In addition, co-culture of neural crest cells differentiated from iPS cells with HPDLCs in the presence of TGF-beta upregulated the expression of periodontal ligament-related markers, suggesting iPS cell differentiation into HPDLC-like cells. Although further studies are required, the combination of iPS cells induced to differentiate into neural crest-like cells and hybrid scaffold could be alternative cell source for novel cell transplantation method to regenerate periodontal ligament tissue.

Research Products

• [Journal Article] Wnt5a induces collagen production by human periodontal ligament cells through TGFβ1-mediated upregulation of Periostin expression (2015)
  • Author(s): Hasegawa D, Wada N, Maeda H, Yoshida S, Mitarai H, Tomokiyo A, Monnouchi S, Hamano S, Yuda A, Akamine A
  • Journal Title: J Cell Physiol. Volume: Epub ahead of print Issue: 11 Pages: 2647-2660
  • DOI: 10.1002/jcp.24950
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Semaphorin 3A induces mesenchymal stem-like properties in human periodontal ligament cells (2014)
  • Author(s): Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Yoshida S, Tomokiyo A, Monnouchi S, Akamine A
  • Journal Title: Stem Cells Dev Volume: (Epub ahead of print) Issue: 18 Pages: 2225-2236
  • DOI: 10.1089/scd.2013.0405
  • Peer Reviewed
• [Journal Article] Regeneration of the periodontium for preservation of the damaged tooth (2014)
  • Author(s): Maeda H, Tomokiyo A, Wada N, Koori K, Kawachi G, Akamine A.
  • Journal Title: Histol Histopathol. Volume: 29 Pages: 1249-1262
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 歯根膜幹細胞の多様な特性およびその有用性 (2014)
  • Author(s): 和田尚久
  • Organizer: 第56回歯科基礎医学会学術大会 サテライトシンポジウム「間葉系幹細胞の直接的/間接的な組織再生への関与を考える」
  • Place of Presentation: 福岡国際会議場（福岡市）
  • Year and Date: 2014-09-25
  • Invited