| Project/Area Number |
25670812
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Conservative dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
HAYASHI Yoshihiko 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (20150477)
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| Co-Investigator(Kenkyū-buntansha) |
山田 志津香 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (00363458)
藤原 守 長崎大学, 医歯薬学総合研究科(歯学系), 客員研究員 (40336178)
井川 一成 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (80584739)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | Caイオンの移動 / 細胞増殖 / 細胞分化 / 骨芽細胞 / Caチャネル / ニフェジピン / Fluo 4-AM / Ca蛍光強度 / ホウ素 / Na+/K+-ATPase / Caイオンチャネル / 細胞膜の活性化 / 石灰化 |
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| Outline of Final Research Achievements |
The aim of this study was to investigate the effects of 0.1 mM of boron (B) on the membrane function of osteoblastic cells in vitro. The Ca2+ flux was evaluated for the activation of L-type Ca2+ channel for the Ca2+ influx, and that of Na+/K+-ATPase for the Ca2+ efflux. A real-time PCR analysis revealed that the expression of four mineralization-related genes was increased with a B-supplemented medium. Using microarray analyses, five genes involved in cell proliferation and differentiation were up-regulated. Regarding the Ca2+ influx, in the nifedipine-pretreated group, the relative fluorescence intensity for one min after adding B solution did not increase compared with that for one min before addition. Regarding the Ca2+ efflux, in 0.1 mM of B-supplemented medium, the relative fluorescence intensity for 10-min after ouabain treatment revealed a significantly lower slope value. B promotes the proliferation and differentiation of mammalian osteoblastic cells in vitro.
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