| Project/Area Number |
25670818
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Prosthodontics/ Dental materials science and
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
KUBOKI Takuo 岡山大学, 医歯(薬)学総合研究科, 教授 (00225195)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ONO Mitsuaki 岡山大学, 大学院医歯薬学総合研究科インプラント再生補綴学分野, 助教 (60613156)
AKIYAMA Kentaro 岡山大学, 大学病院クラウン・ブリッジ補綴科, 講師 (70423291)
園山 亘 岡山大学, 大学病院, 講師 (40325121)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | microRNA / リプログラミング / side population / 間葉系幹細胞 / Side population |
|---|
| Outline of Final Research Achievements |
Small non-coding microRNAs (miRNAs) have been reported to play important roles in stem cell biology, related to cell reprogramming, maintenance of stemness and regulation of cell differentiation. We herein sorted stem-cell-enriched side population (SP) cells from human DPCs and PDLCs, and performed a miRNA array. As a result, miR-720 was highly expressed in the differentiated main population (MP) cells compared to that in SP cells. In silico analysis and a reporter assay showed that miR-720 targets the stem cell marker NANOG, indicating that miR-720 could promote differentiation of dental pulp stem/ progenitor cells by repressing NANOG. Indeed, gain-and loss-of-function analyses showed that miR-720 controls NANOG transcript and protein levels. Moreover, transfection of miR-720 significantly decreased the number of cells positive for the early stem cell marker SSEA-4. Our findings identify miR-720 as a novel miRNA regulating the differentiation of DPCs.
|
