Development of new approach for effective bone repair using small natural compounds and sustained-relesing nanogel

• Project/Area Number: 25670831
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Japan Women's University
• Principal Investigator: OTA Masato 日本女子大学, 家政学部, 准教授 (70313228)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 天然低分子化合物 / ホノキオール / 効率的骨修復法 / 徐放性ゲル / nanogel / Bmp２ / 個体差の解消 / 超高齢化社会 / 低分子化合物 / ハルミン / 骨芽細胞分化 / BMP２ / 効率的骨形成 / CHPAナノゲル / honokiol / BMP－2

Outline of Final Research Achievements

Accompanied by the rapid progress of the super-aging society , the risk of osteoporosis and/or bone fracture, which is related to degradation of quality of life or secondary iatrogenic disease due to long-term hospitalization, is becoming a social problem. To prevent this problem,development of efficient bone regeneration method is an very important and urgent issue for the Japanese people in general. We have developed a new bone regeneration method which combined the honokiol,extracted from magnolia that has been used since ancient times in Japnease medicine,and BMP2 protein and applied to a bone defect using nanogels.This method eliminated the individual differences , yet efficiently to promote bone regeneration. Further, in the present project , we conducted the development of in vitro or in vivo assay systems to analyze the detailed functional mechanism of new bone regeneration method including Honokiol.

Research Products

• [Journal Article] Foxc1 is required for early stage telencephalic vascular development. (2015)
  • Author(s): Prasitsak T, Nandar M, Okuhara S, Ichinose S, Ota MS, Iseki S.
  • Journal Title: Dev Dyn. Volume: 244 Issue: 5 Pages: 703-711
  • DOI: 10.1002/dvdy.24269
  • Peer Reviewed
• [Journal Article] Excess NF-kB induces ectopic odontogenesis in embryonic incisor epithelium. (2015)
  • Author(s): Blackburn, J., Kawasaki, K., Porntaveetus, T., Kawasaki, M., Otsuka-Tanaka, Y., Miake, Y., Ota, M.S., Watanabe, M., Hishinuma, M., Nomoto, T., Oommen, S., Ghafoor, S., Harada, F., Nozawa-Inoue, K., Maeda, T., Peterková, R., Lesot, H., Inoue, J., Akiyama, T.,
  • Journal Title: J. Dent. Res Volume: 94 Issue: 1 Pages: 121-128
  • DOI: 10.1177/0022034514556707
  • Peer Reviewed / Open Access
• [Journal Article] Comparison of stress-induced modulation of smooth-muscle activity between ileum and colon in male rats (2014)
  • Author(s): Mari Kimoto,Jorge L Zeredo,Masato S Ota, Zenro Nihe, Kazuo Toda
  • Journal Title: Autonomic Neuroscience Volume: - Pages: 8-11
  • DOI: 10.1016/j.autneu.2014.01.008
  • Peer Reviewed
• [Journal Article] Expression of transcripts for fibroblast growth factor 18 and its possible receptors during postnatal dentin formation in rat molars. (2014)
  • Author(s): Otto Baba. Masato S Ota, Tatsuo Terashima, Makoto J Tabata, Yoshiro Takano
  • Journal Title: Odontology Volume: 102 Issue: 2 Pages: 1-5
  • DOI: 10.1007/s10266-013-0147-9
  • Peer Reviewed
• [Journal Article] FGF18 accelerates osteoblast differentiation by upregulating Bmp2 expression. (2013)
  • Author(s): Nagayama T, Okuhara S, Ota MS, Tachikawa N, Kasugai S, Iseki S.
  • Journal Title: Congenit Anom (Kyoto). Volume: 53 Issue: 2 Pages: 83-8
  • DOI: 10.1111/cga.12012
  • Peer Reviewed