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Bone regeneration with bone substitute and cells in osteoclast lineage

Research Project

Project/Area Number 25670834
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Dental engineering/Regenerative dentistry
Research InstitutionNagasaki University

Principal Investigator

IKEDA Tohru  長崎大学, 医歯薬学総合研究科(歯学系), 教授 (00211029)

Co-Investigator(Kenkyū-buntansha) NISHIMURA Masahiro  鹿児島大学, 医歯学総合研究科(歯学系), 教授 (00294570)
KAMITAKAHARA Masanobu  東北大学, 環境科学研究科, 准教授 (80362854)
IOKU Koji  慶応義塾大学, 経済学部, 教授 (60212726)
Co-Investigator(Renkei-kenkyūsha) ERI Tatsukawa  長崎大学, 医歯薬学総合研究科(歯学系), 技術職員 (60534534)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords骨欠損 / 再生医療 / 破骨細胞 / 異物巨細胞 / 肉芽腫 / 酒石酸抵抗性酸性ホスファターゼ / カテプシンK / 骨代謝 / 骨形成 / 骨芽細胞 / 幹細胞 / 骨代替材料
Outline of Final Research Achievements

We tried to show importance of osteoclasts for bone regeneration in the region implanted with bone substitutes and osteoblast progenitor cells. We implanted granules of biodegradable bone substitute, beta tricalcium phosphate (β-TCP) in rat subcutaneous tissue with or without bone marrow mesenchymal cells, which include osteoblast progenitor cells. Implantation of bone marrow mesenchymal cells induced not only osteogenesis, but also osteoclastogenesis. Osteoclastogenesis stimulated resorption of β-TCP. These results showed that implantation of bone marrow mesenchymal cells stimulated replacement of bone substitute to the bone. In addition, we found that hydroxyapatite composed of rod shaped particles induced osteoclast-like multinucleated giant cells without implantation of bone marrow mesenchymal cells. Furthermore, we showed divergence of multinucleated giant cells induced around hydroxyapatite granules implanted in the rat subcutaneous tissue.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2015

All Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] セラミック皮下移植系を用いた破骨細胞及び異物巨細胞形成機構の検討2015

    • Author(s)
      森下廣太,柴田恭明,Gazi Jased Ahmed,梅田正博,池田 通
    • Organizer
      第26回日本臨床口腔病理学会
    • Place of Presentation
      北海道大学学術交流会館(北海道・札幌市)
    • Year and Date
      2015-07-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] セラミック皮下移植実験系による骨芽細胞非存在下での破骨細胞形成2015

    • Author(s)
      森下 廣太、柴田 恭明、立川 絵里、上高原 理暢、Gazi Jased Ahmed、梅田 正博、池田 通
    • Organizer
      第33回日本骨代謝学会
    • Place of Presentation
      京王プラザホテル(東京都)
    • Year and Date
      2015-07-23 – 2015-07-25
    • Related Report
      2014 Research-status Report
  • [Presentation] セラミック皮下移植実験系による骨芽細胞非存在下での破骨細胞形成2015

    • Author(s)
      森下廣太,柴田恭明,立川絵里,上高原理暢,Gazi Jased Ahmed,梅田正博,池田 通
    • Organizer
      第33回日本骨代謝学会学術集会
    • Place of Presentation
      京王プラザホテル(東京都・新宿区)
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] Functional hydroxyapatite ceremics prepared by hydrothermal processing2015

    • Author(s)
      Ioku K, Kawagoe D, Fujimori H, Kamitakahara M, Ikeda T.
    • Organizer
      Biomaterials International Conference 2015
    • Place of Presentation
      Kenting (Taiwan)
    • Year and Date
      2015-06-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

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Published: 2014-07-25   Modified: 2019-07-29  

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