Bone regeneration with bone substitute and cells in osteoclast lineage

• Project/Area Number: 25670834
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Nagasaki University
• Principal Investigator: IKEDA Tohru 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (00211029)
• Co-Investigator(Kenkyū-buntansha): NISHIMURA Masahiro 鹿児島大学, 医歯学総合研究科(歯学系), 教授 (00294570) ; KAMITAKAHARA Masanobu 東北大学, 環境科学研究科, 准教授 (80362854) ; IOKU Koji 慶応義塾大学, 経済学部, 教授 (60212726)
• Co-Investigator(Renkei-kenkyūsha): ERI Tatsukawa 長崎大学, 医歯薬学総合研究科(歯学系), 技術職員 (60534534)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 骨欠損 / 再生医療 / 破骨細胞 / 異物巨細胞 / 肉芽腫 / 酒石酸抵抗性酸性ホスファターゼ / カテプシンK / 骨代謝 / 骨形成 / 骨芽細胞 / 幹細胞 / 骨代替材料

Outline of Final Research Achievements

We tried to show importance of osteoclasts for bone regeneration in the region implanted with bone substitutes and osteoblast progenitor cells. We implanted granules of biodegradable bone substitute, beta tricalcium phosphate (β-TCP) in rat subcutaneous tissue with or without bone marrow mesenchymal cells, which include osteoblast progenitor cells. Implantation of bone marrow mesenchymal cells induced not only osteogenesis, but also osteoclastogenesis. Osteoclastogenesis stimulated resorption of β-TCP. These results showed that implantation of bone marrow mesenchymal cells stimulated replacement of bone substitute to the bone. In addition, we found that hydroxyapatite composed of rod shaped particles induced osteoclast-like multinucleated giant cells without implantation of bone marrow mesenchymal cells. Furthermore, we showed divergence of multinucleated giant cells induced around hydroxyapatite granules implanted in the rat subcutaneous tissue.

Research Products

• [Presentation] セラミック皮下移植系を用いた破骨細胞及び異物巨細胞形成機構の検討 (2015)
  • Author(s): 森下廣太，柴田恭明，Gazi Jased Ahmed，梅田正博，池田 通
  • Organizer: 第26回日本臨床口腔病理学会
  • Place of Presentation: 北海道大学学術交流会館(北海道・札幌市)
  • Year and Date: 2015-07-29
• [Presentation] セラミック皮下移植実験系による骨芽細胞非存在下での破骨細胞形成 (2015)
  • Author(s): 森下 廣太、柴田 恭明、立川 絵里、上高原 理暢、Gazi Jased Ahmed、梅田 正博、池田 通
  • Organizer: 第33回日本骨代謝学会
  • Place of Presentation: 京王プラザホテル（東京都）
  • Year and Date: 2015-07-23 – 2015-07-25
• [Presentation] セラミック皮下移植実験系による骨芽細胞非存在下での破骨細胞形成 (2015)
  • Author(s): 森下廣太，柴田恭明，立川絵里，上高原理暢，Gazi Jased Ahmed，梅田正博，池田 通
  • Organizer: 第33回日本骨代謝学会学術集会
  • Place of Presentation: 京王プラザホテル(東京都・新宿区)
  • Year and Date: 2015-07-23
• [Presentation] Functional hydroxyapatite ceremics prepared by hydrothermal processing (2015)
  • Author(s): Ioku K, Kawagoe D, Fujimori H, Kamitakahara M, Ikeda T.
  • Organizer: Biomaterials International Conference 2015
  • Place of Presentation: Kenting (Taiwan)
  • Year and Date: 2015-06-01
  • Int'l Joint Research