Development of a novel cell and tissue freezing technology using variable magnetic field

• Project/Area Number: 25670837
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Matsumoto Dental University
• Principal Investigator: KAGAMI Hideaki 松本歯科大学, 歯学部, 教授 (80242866)
• Co-Investigator(Kenkyū-buntansha): SAGARA Hajime 東京大学, 医科学研究所, 助教 (50145041) ; 李 憲起 松本歯科大学, 総合歯科医学研究所, 講師 (60350831)
• Co-Investigator(Renkei-kenkyūsha): LI Kenki 松本歯科大学, 総合歯科医学研究所, 講師 (60350831)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 凍結保存法 / 凍結生物学 / 細胞 / 再生医療 / 解凍 / 凍結保存 / 培養細胞 / 変動磁場 / 幹細胞 / 組織

Outline of Final Research Achievements

For the wide acceptance of regenerative medicine, the development of cell transportation technology is important. However, currently available technologies are not capable to transport living cells for a long distance. Accordingly, freezing is a promissing approach to solve this problem. Tissue engineering utilize cells from patient or donors. Harvesting cells at each treatment is a burden for patients. Cell freezing technology is also beneficial for the stable supply of stem cells. In this study, freezin technology from food industry was introduced and modified to achieve higher living ratio after feeze-thaw cycle. Furthermore, we also investigated the usefulness of direct tissue freezing strategy, which enables cultivation of stem cells from frozen tissue in the future.

Research Products

• [Journal Article] The potential use of cell-based therapies in the treatment of oral diseases. (2015)
  • Author(s): Kagami H
  • Journal Title: Oral Diseases Volume: in press Issue: 5 Pages: 545-549
  • DOI: 10.1111/odi.12320
  • Peer Reviewed
• [Journal Article] Characterization of time-course morphological features for efficient prediction of osteogenic potential in human mesenchymal stem cells. (2014)
  • Author(s): F. Matsuoka, I. Takeuchi, H. Agata, H. Kagami, H. Shiono H, Y. Kiyota, H. Honda, R. Kato
  • Journal Title: Biotechnol. Bioeng. Volume: epub Issue: 7 Pages: 1430-1439
  • DOI: 10.1002/bit.25189
  • Peer Reviewed
• [Journal Article] The use of bone marrow stromal cells (bone marrow-derived multipotent mesenchymal stromal cells) for alveolar bone tissue engineering: basic science to clinical translation. (2014)
  • Author(s): Kagami H, Agata H, Inoue M, Asahina I, Tojo A, Yamashita N. Imai K.
  • Journal Title: Tissue Eng. Part B Volume: in press
  • NAID: 120006986575
  • Peer Reviewed
• [Journal Article] GDFs promote tenogenic characteristics on human periodontal ligament-derived cells in culture at late passages (2013)
  • Author(s): Xia D, Sumita Y, Liu Y, Tai Y, Wang J, Uehara M, Agata H, Kagami H, Fan Z, Asahina I, Wang S, Tran SD
  • Journal Title: Growth Factors Volume: vol.31(5) Issue: 5 Pages: 165-73
  • DOI: 10.3109/08977194.2013.830611
  • Peer Reviewed
• [Journal Article] Morphology-based prediction of osteogenic differentiation potential of human mesenchymal stem cells (2013)
  • Author(s): Matsuoka F et al.
  • Journal Title: Plos One Volume: 8 Issue: 2 Pages: e55082-e55082
  • DOI: 10.1371/journal.pone.0055082
  • Peer Reviewed
• [Journal Article] Ischemic culture of dental pulp-derived cells is a useful model in which to investigate mechanisms of post-ischemic tissue recovery. (2013)
  • Author(s): Agata A, Sumita Y, Asahina I, Tojo A, Kagami A,
  • Journal Title: Histol Histopathol. Volume: 28 Pages: 985-991
  • Peer Reviewed
• [Presentation] Bone marrow stromal cells (bone marrow-derived multipotent mesenchymal stromal cells) for alveolar bone tissue engineering: basic science to clinical translation.
  • Author(s): Kagami H
  • Organizer: Biobridge. Generation Regeneration Conference.
  • Place of Presentation: Giorgio Cini Foundation, Venice, Italy.
  • Invited
• [Book] Optimization of stem cell expansion, storage, and distribution. Chapter 25, in “Stem Cell Biology and Tissue engineering in Dental Science” (2014)
  • Author(s): Kagami H
  • Publisher: Elsevier
• [Book] "New Trends in Tissue Engineering and Regenerative Medicine - Official Book of the Japanese Society for Regenerative Medicine" (2014)
  • Author(s): Sumita Y, Asahina I, Tran SD, Agata H, Inoue M, Tojo A, Kagami H
  • Publisher: Intech