Effect of the cells transformed by elastins on facilitation of DNA/protamine complex-induced bone regeneration
| Project/Area Number |
25670838
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OHNO Jun 福岡歯科大学, 歯学部, 講師 (10152208)
MORI Nana 福岡歯科大学, 歯学部, 助教 (60736677)
SHINOZAKI Yosuke 福岡歯科大学, 歯学部, 助教 (80736687)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | エラスチン / ＤＮＡ / プロタミン / 骨再生 / ペースト / DNA / 細胞形質転換 / 細胞形質転換機能 / 骨補填材 / 骨形成 |
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| Outline of Final Research Achievements |
t was reported that fibroblast cell can differentiate into myofibroblast cell via osetoblast cell in the presence of elaschin peptide. Thus, three mixtures of DNA/protamine complex with salmon-derived water-insoluble elaschin, water-soluble elastin and eraschin peptide were prepared. These pastes were implanted into rat cranial defect for three months. It was found that the DNA/protamine complex with elaschin peptide formed new bone faster than the others. Moreover, real-time PCR indicated that elaschin peptide can facilitate the development of αーSMA and MMP-2. Therefore, elaschin peptide will be useful materials for repair of bone defects.
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Report
(3 results)
Research Products
(8 results)
