Functional analysis and clinical application of let-7 microRNA in oral cancer

• Project/Area Number: 25670844
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: University of Tsukuba
• Principal Investigator: BUKAWA HIROKI 筑波大学, 医学医療系, 教授 (80173558)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: microRNA / let-7 / 口腔がん / 腫瘍生物学 / 唾液 / 血液

Outline of Final Research Achievements

We revealed that let-7c microRNA and the let-7g microRNA expression is significantly reduced as compared to normal tissue in the oral cancer tissue. Furthermore we also revealed that the expression of let-7c and let-7g is significantly reduced as compared to normal skin cell lines in cell line (HSC3) derived human oral carcinoma. In addition, we made it clear that the expression level of these two microRNA is correlated with cervical lymph node metastasis of oral cancer. Finally we have found that the addition of an agonist of the let-7c and let-7g to HSC3 may inhibit cancer cell growth ability.

Research Products

• [Presentation] 口腔がんにおけるmiRNA let-7 family 発現の意義 (2015)
  • Author(s): 長井宏樹 , 長谷川正午, 馬場脩 , 内田文彦 , 寺邊健人 , 菅野直美 , 大和地正信 , 山縣憲司 , 柳川徹 , 武川寛樹
  • Organizer: 第33回日本口腔腫瘍学会総会・学術大会
  • Place of Presentation: 奈良
  • Year and Date: 2015-01-29 – 2015-01-30
• [Presentation] 口腔癌の頸部リンパ節転移におけるmiR-205 とInterferon Regulatory Factor 1 の関係 (2014)
  • Author(s): 長谷川正午, 内田文彦, 馬場 脩, 長井宏樹, 伊藤孝明, 大和地正信, 菅野直美, 山縣憲司, 柳川 徹, 武川寛樹
  • Organizer: 第59回公益社団法人日本口腔外科学会総会・学術大会
  • Place of Presentation: 千葉
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] 口腔癌の頸部リンパ節転移におけるmiR-155-5p とzinc finger protein 703 の関係 (2014)
  • Author(s): 内田文彦, 長谷川正午, 馬場 脩, 寺邊健人, 伊藤孝明, 大和地正信, 菅野直美, 山縣憲司, 柳川 徹, 武川寛樹
  • Organizer: 第59回公益社団法人日本口腔外科学会総会・学術大会
  • Place of Presentation: 千葉
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] 口腔がんにおけるmiR-203 の機能解析と臨床応用 (2014)
  • Author(s): 馬場 脩, 伊藤孝明, 内田文彦, 大和地正信, 菅野直美, 山縣憲司, 長谷川正午, 柳川 徹, 鬼澤浩司郎, 武川寛樹
  • Organizer: 第59回公益社団法人日本口腔外科学会総会・学術大会
  • Place of Presentation: 千葉
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] miR-205-5p Targets Interferon Regulatory Factor 1 and Suppresses Metastasis in Oral Cancer Cells (2014)
  • Author(s): Shogo Hasegawa, Fumihiko Uchida, Osamu Baba, Takaaki Ito, Masanobu Yamatoji, Naomi I. Kanno, Kenji Yamagata, Toru Yanagawa, Hiroki Bukawa
  • Organizer: 2014 AAOMS Annual Meeting - American Association of Oral and Maxillofacial Surgeons
  • Place of Presentation: ホノルル(USA)
  • Year and Date: 2014-09-08 – 2014-09-13
• [Presentation] miRNA-155-5p Targets ZNF703 and Suppresses Metastasis in Oral Cancer Cells (2014)
  • Author(s): Fumihiko Uchida, Shogo Hasegawa, Osamu Baba, Takaaki Ito, Masanobu Yamatoji, Naomi I. Kanno, Kenji Yamagata, Toru Yanagawa, Hiroki Bukawa
  • Organizer: 2014 AAOMS Annual Meeting - American Association of Oral and Maxillofacial Surgeons
  • Place of Presentation: ホノルル(USA)
  • Year and Date: 2014-09-08 – 2014-09-13
• [Presentation] Utility of Saliva in the Evaluation of Microrna Functions as a Tumor Suppressor in Oral Squamous Cell Carcinoma (2014)
  • Author(s): Osamu Baba, Takaaki Ito, Fumihiko Uchida, Masanobu Yamatoji, Naomi I. Kanno, Kenji Yamagata, Shogo Hasegawa, Toru Yanagawa, Hiroki Bukawa
  • Organizer: 2014 AAOMS Annual Meeting - American Association of Oral and Maxillofacial Surgeons
  • Place of Presentation: ホノルル(USA)
  • Year and Date: 2014-09-08 – 2014-09-13
• [Presentation] 血清・唾液をサンプルとした口腔がん関連遊離microRNAの網羅的発現解析
  • Author(s): 内田文彦、大和地正信、瀬戸佳穂里、馬場 脩、伊藤寛之、山縣憲司、柳川 徹、武川寛樹
  • Organizer: 第67回 日本口腔科学会学術会
  • Place of Presentation: 栃木県総合分化センター（栃木県）