Investigation of factors which simultaneously regulate bone formation and bone resorption in terms of cell cycle regulation.

• Project/Area Number: 25670846
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: The University of Tokyo
• Principal Investigator: OGASAWARA TORU 東京大学, 医学部附属病院, 講師 (20359623)
• Co-Investigator(Kenkyū-buntansha): 緒方 直史 帝京大学, 医学部, 教授 (10361495) ; 阿部 雅修 東京大学, 保健・健康推進本部, 講師 (10392333) ; 安部 貴大 東京大学, 医学部附属病院, 特任講師 (20383250) ; 筑田 博隆 東京大学, 医学部附属病院, 講師(移行) (30345219) ; 井口 隆人 東京大学, 医学部附属病院, 助教 (80587775)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 再生医学 / 骨代謝学 / 細胞周期 / 細胞分化 / 幹細胞 / 骨形成 / 骨吸収 / 骨軟骨分化 / 骨再生

Outline of Final Research Achievements

This study aimed to discover factors which simultaneously regulate bone formation and bone resorption, in terms of cell cycle regulation. DNA microarray analysis and Real time quantitative PCR analysis etc. revealed that Runx1, Runx3, Cyclin D1 regulate different cellular processes simultaneously, including bone and cartilage metabolism. In addition, we discovered factors which were up-regulated in osteoclast differentiation, while down-regulated in osteoblast differentiation. We also discovered factors which were down-regulated in osteoclast differentiation, while up-regulated in osteoblast differentiation. In conclusion, the results of this study suggested that some of the cell cycle factors simultaneously regulate genes involving in bone and cartilage metabolism.

Research Products

• [Journal Article] Runx1 and Runx3 Are Downstream Effectors of Nanog in Promoting Osteogenic Differentiation of the Mouse Mesenchymal Cell Line C3H10T1/2 (2015)
  • Author(s): Saito T, Ohba S, Yano F, Seto I, Yonehara Y, Takato T, Ogasawara T
  • Journal Title: Cell Reprogram Volume: 17 Issue: 3 Pages: 227-234
  • DOI: 10.1089/cell.2014.0059
  • Peer Reviewed / Open Access
• [Presentation] CyclinD1はNanogが有する間葉系細胞骨軟骨分化能促進効果のバランスを制御する (2017)
  • Author(s): 小笠原 徹
  • Organizer: 第16回日本再生医療学会総会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2017-03-07
• [Presentation] CyclinD1 regulates the balance between the osteogenic and chondrogenic differentiation of mesenchymal cells that constitutively express Nanog. (2016)
  • Author(s): Toru Ogasawara, Jumpei Imamura, Yasuyuki Fujii
  • Organizer: ASBMR 2016 Annual Meeting
  • Place of Presentation: Atlanta, Georgia, USA, Georgia World Congress Center
  • Year and Date: 2016-09-16
  • Int'l Joint Research
• [Presentation] Nanogの間葉系細胞骨分化能促進効果におけるRunxファミリー（Runx1/2/3）の関与 (2015)
  • Author(s): 小笠原徹
  • Organizer: 第68回NPO法人日本口腔科学会学術大会
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2015-05-08 – 2015-05-09
• [Presentation] Runx1 and Runx3 are downstream effectors of Nanog in the promoted osteogenic differentiation of mesenchymal cells. (2013)
  • Author(s): Toru Ogasawara et al.
  • Organizer: 2013 annual meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: Baltimore, Maryland, USA, Baltimore Convention Center
• [Remarks] 東大口腔外科HP業績
  • URL: https://plaza.umin.ac.jp/~oralsurg/archive.html
• [Remarks] 東京大学医学部附属病院 顎口腔外科・歯科矯正歯科ホームページ
  • URL: http://plaza.umin.ac.jp/~oralsurg/
• [Remarks] 東京大学医学部附属病院顎口腔外科・歯科矯正歯科ホームページ
  • URL: http://plaza.umin.ac.jp/~oralsurg/archive.html
• [Remarks] 東京大学医学部附属病院 顎口腔外科・歯科矯正歯科ホームページ
  • URL: http://plaza.umin.ac.jp/~oralsurg/