The development of the injectable hydrogel including periosteum stem cells to increase the volume of the alveolar bone

• Project/Area Number: 25670849
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: The University of Tokyo
• Principal Investigator: UCHINO Natsuko 東京大学, 医学部附属病院, 助教 (30569637)
• Co-Investigator(Kenkyū-buntansha): KAZUMI Ohkubo 東京大学, 医学部附属病院, 講師 (10396715)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 歯槽骨再建 / 幹細胞 / ペプチドハイドロゲル

Outline of Final Research Achievements

We have focused on periosteum of mentum as an abundant source of stem cell for regeneration of oral tissues. To develop the injectable hydrogel includes the cultured stem cells based on the biocompatible self-organization peptide hydrogel ‘‘PuraMatrix’’, we have evaluated the culture method and differentiation method for maintaining pluripotency of stem cell derived from human or rat. As results, the periosteum stem cells maintained stem cell properties in comparison with cells of surrounding oral tissue. In addition, we have prepared the bone defect model and extraction socket model then implanted stem cells derived from synovium to the defect. By the animal experiments, bone regeneration was observed the abundant extracellular matrix histologically. It was clear that the promotion of bone formation was enabled by using the injectable hybrid hydrogel easily, noninvasively. In this project, it was revealed that it was an effective method for relatively a little bone defect case.

Research Products

• [Journal Article] Incisor Inclination after Presurgical Orthodontic Treatment in Patients with Mandibular Prognathism (2014)
  • Author(s): Ohkubo K, Susami T, Inokuchi T, Okayasu M, Takahashi N, Uwatoko K, Uchino N, Suenaga H, Koga Y, Saijo H, Mori Y and Takato T
  • Journal Title: The Japanese Journal of Jaw Deformities Volume: 24 Issue: 1 Pages: 16-26
  • DOI: 10.5927/jjjd.24.16
  • NAID: 130003397166
  • ISSN: 0916-7048, 1884-5045
  • Peer Reviewed / Open Access
• [Journal Article] Fabrication of Stereotyped Beta-Tricalcium-Phosphate Blocks into a Conjugated Structure using Mesenchymal Stem Cell Sheets Prepared in Temperature-Responsive Culture Dishes. J of Hard Tissue Biology. (2014)
  • Author(s): Mori Y, Fujihara Y, Misawa M, Inoue H, Inaki R, Suenaga H, Okubo K, Saijo H, Takato T, Hoshi K
  • Journal Title: J of Hard Tissue Biology Volume: 印刷中
• [Presentation] 先端巨大症に伴う下顎前突を外科的矯正治療により改善した２症例 (2014)
  • Author(s): 井口隆人, 須佐美隆史, 長濱浩平, 内野夏子, 阿部雅修, 末永英之, 森良之, 髙戸毅
  • Organizer: 第24回日本顎変形症学会総会・学術大会
  • Place of Presentation: アクロス福岡
  • Year and Date: 2014-06-10 – 2014-06-11
• [Presentation] 唇顎口蓋裂患者の混合歯列における口蓋形態・上下顎骨関係と矯正歯科治療との関連性 (2014)
  • Author(s): 井口隆人, 須佐美隆史, 大久保和美, 岡安麻里, 内野夏子, 上床喜和子, 高橋直子, 末永英之, 髙戸毅
  • Organizer: 第38回日本口蓋裂学会学術大会・総会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-05-29 – 2014-05-30
• [Presentation] 先天異常患者における疾患別平均側面頭部X線規格写真トレース像の作成 (2013)
  • Author(s): 内野夏子, 須佐美隆史, 井口隆人, 岡安麻里, 大久保和美, 上床喜和子, 高橋直子, 森良之, 高戸 毅
  • Organizer: 第72回日本矯正歯科学会大会
  • Place of Presentation: 長野キッセイ文化ホール