| Project/Area Number |
25670854
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
KENJI SEO 新潟大学, 医歯学系, 教授 (40242440)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Takeyasu 新潟大学, 医歯学系, 教授 (40183941)
IDA Hiroko 新潟大学, 医歯学系, 准教授 (60293213)
FUJIWARA Naoshi 新潟大学, 医歯学系, 教授 (70181419)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | BONE / BDNF / OSTEOBLAST / OSTEOCLAST / REMODELING / NERVE INJURY / TRIGEMINAL NERVE / MANDIBLE / 三叉神経 / 神経損傷 / 破骨細胞 / リモデリング / TrkB / 下歯槽神経 / 下顎管 / 骨吸収 / 前骨芽細胞 / NGF / 細胞増殖 / 遺伝子発現 / 細胞遊走性試験 |
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| Outline of Final Research Achievements |
BDNF is known to promote the natural healing of injured nerves, but it also induces sclerotic changes in bone. We reported that peripheral nerve injury promotes the local production of BDNF. Therefore, this study aimed to evaluate the effect of local BDNF on osteogenesis. Results: BDNF significantly activated the mRNA expression of osteopontin and osteocalcin in MC3T3-E1 cells without affecting cell proliferation. The promotion of the differentiation of MC3T3-E1 cells by BDNF was predicted to occur via the activation of Akt signaling through trkB. Osteopontin-positive new bone formation on the surface of preexisting bone was significantly accelerated in the BDNF-grafted groups, and active bone remodeling, involving osteoblasts, osteoclasts and osteocytes, continued after 28 days due to exogenous BDNF. Conclusion: Local BDNF produced by inferior alveolar nerve injury in the mendible can contribute to accelerating osteosclerotic changes in the alveolar bone.
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