Bone disease analysis using human disease-specific iPS cells.

• Project/Area Number: 25670856
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Kyoto University
• Principal Investigator: BESSHO Kazuhisa 京都大学, 医学(系)研究科(研究院), 教授 (90229138)
• Co-Investigator(Kenkyū-buntansha): TAKAHASHI Katsu 京都大学, 医学研究科, 准教授 (90314202) ; NAKAO Kazumasa 京都大学, 医学研究科, 助教 (40599932) ; KOYAMA Noriaki 京都大学, 医学研究科, 客員研究員 (30599931)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 疾患特異的iPS細胞 / GNAS遺伝子変異 / 線維性異形成症 / （１） 疾患特異的iPS細胞 / （２） GNAS遺伝子変異 / （３） 線維性異形成症

Outline of Final Research Achievements

We will investigate pathological analysis using human FD（Fibrous Dysplasia; FD） cells which is the representative for preparation of bone disease.
We collected FD lesion from FD patients and we cultured human FD cells. Although we could get wild-type iPS cells, we couldn't establish human iPS cells from the FD cells mutation.

Research Products

• [Journal Article] Schwannoma Located in the Upper Gingival Mucosa: Case Report and Literature Review (2015)
  • Author(s): 9.Emi Nishijima Sakanashi, Junya Sonobe, Motofumi Chin, Kazuhisa Bessho
  • Journal Title: Journal of Maxillofacial and Oral Surgery Volume: 14 Issue: S1 Pages: 222-225
  • DOI: 10.1007/s12663-012-0445-8
  • Peer Reviewed
• [Journal Article] Bone regeneration of rat calvarial defect by magnesium calcium phosphate gelatin scaffolds with or without bone morphogenetic protein-2 (2014)
  • Author(s): A. Hussain, K. Takahashi, J. Sonobe, M Bamba, Y. Tabata, K. Bessho
  • Journal Title: J Maxillofac Oral Surg Volume: 13 Issue: 1 Pages: 29-35
  • DOI: 10.1007/s12663-013-0478-7
  • Peer Reviewed
• [Journal Article] Recent Clinical Recent Clinical Evidence in Bisphosphonate-Related Osteomyelitis of the Jaw: Focus on Risk, Prevention and Treatment (2014)
  • Author(s): Toru Yamazaki, Katsu Takahashi, Kazuhisa Bessho
  • Journal Title: Review on Recent Clinical Trial Volume: 9 Issue: 1 Pages: 37-52
  • DOI: 10.2174/1574887109666140423120614
  • Peer Reviewed / Open Access
• [Journal Article] Prospective signs of cleidocranial dysplasia in Cebpb deficiency (2014)
  • Author(s): Boyen Huang1,2, Katsu Takahashi2*, Ernest A Jennings1 , Pongthorn Pumtang-on3 , Honoka Kiso2 , Yumiko Togo2 , Kazuyuki Saito2 , Manabu Sugai4 , Shizuo Akira5 , Akira Shimizu4 and Kazuhisa Bessho2
  • Journal Title: Journal of Biomedical Science Volume: 21 Issue: 1 Pages: 44-48
  • DOI: 10.1186/1423-0127-21-44
  • Peer Reviewed / Open Access
• [Journal Article] The effect of nicotine on osteoinduction by recombinant human bone morphogenetic protein-2. (2014)
  • Author(s): Tamura, K., Togo, Y., Kaihara, S., Hussain, A., Takahashi, K., and Bessho, K.
  • Journal Title: Int J Oral Maxillofac Surg Volume: 43 Issue: 8 Pages: 1022-1029
  • DOI: 10.1016/j.ijom.2014.02.014
  • NAID: 10018954979
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Interactions between BMP-7 and USAG-1 (Uterine Sensitization-Associated Gene-1) Regulate Supernumerary Organ Formations (2014)
  • Author(s): 10.Honoka Kiso, Katsu Takahashi, Kazuyuki Saito, Yumiko Togo, Hiroko Tsukamoto, Boyen Huang, Manabu Sugai, Akira Shimizu, Yasuhiko Tabata, Aris N Economides, Harold C Slavkin, Kazuhisa Bessho
  • Journal Title: PLoS ONE Volume: 9
  • Peer Reviewed / Open Access
• [Journal Article] Dental Trauma related to General Anesthesia: Should the Anesthesiologist Perform a Preanesthetic Dental Evaluation? (2014)
  • Author(s): 12.Saeed Riad Idrees, Kazuma Fujimura, Kazuhisa Bessho
  • Journal Title: Oral Health & Dental Management Volume: 13 Pages: 271-274
  • Peer Reviewed
• [Journal Article] An association between anti-nuclear antibodies and HLA class II locus and heterogeneous characteristics of staining patterns: The Nagahama Study. (2014)
  • Author(s): Terao C, Ohmura K, Yamada R, Kawaguchi T, Shimizu M, Tabara Y, Takahashi M, Setoh K, Nakayama T, Kosugi S, Sekine A, Matsuda F, Tsuneyo Mimori T on behalf of the Nagahama Study Group.
  • Journal Title: Arthritis Rheumatol. Volume: 66 Issue: 3_suppl Pages: 3395-403
  • DOI: 10.1177/0022034514559127
  • Peer Reviewed / Open Access
• [Journal Article] Human induced pluripotent stem cells differentiated into chondrogenic lineage via generation of mesenchymal progenitor cells (2013)
  • Author(s): Koyama, N., Miura, M., Nakao, K., Kondo, E., Fujii, T., Taura, D., Kanamoto, N., Sone, M., Yasoda, A., Arai, H., Bessho, K., Nakao, K.
  • Journal Title: Stem Cells and Development Volume: 22(1) Issue: 1 Pages: 102-113
  • DOI: 10.1089/scd.2012.0127
  • Peer Reviewed
• [Journal Article] Aldehyded dextran and ε-poly(l-lysine) hydrogel as non-viral gene carrier. (2013)
  • Author(s): Togo Y, Takahashi K, Saito K, Kiso H, Huang B, Tsukamoto H, Hyon SH, Bessho K.
  • Journal Title: Stem Cell Int, Volume: 2013 Pages: 634379-634379
  • DOI: 10.1155/2013/634379
  • Peer Reviewed
• [Journal Article] Risk factors and indices of osteomyelitis of the jaw in osteoporosis patients: results from a hospital-based cohort study in Japan. (2013)
  • Author(s): Yamazaki T, Yamori M, Tanaka S, Yamamoto K, Sumi E, Nishimoto-Sano M, Asai K, Takahashi K, Nakayama T, Bessho K.
  • Journal Title: PLoS One Volume: 8(11) Issue: 11 Pages: e79376-e79376
  • DOI: 10.1371/journal.pone.0079376
  • Peer Reviewed
• [Journal Article] Methotrexate-associated lymphoproliferative disorder mimicking pyocyanic ecthyma gangrenosum in a patient with rheumatoid arthritis (2013)
  • Author(s): Inuzuka K, Endo Y, Kato M, Fujisawa A, Tanioka M, Kabashima K, Tsukamoto H, Sonobe J, Bessho K, Miyachi Y.
  • Journal Title: Eur J Dermatol. Volume: 23 Issue: 3 Pages: 418-419
  • DOI: 10.1684/ejd.2013.2044
  • Peer Reviewed
• [Journal Article] The effects of C-type natriuretic peptide on craniofacial skeletogenesis (2013)
  • Author(s): Nakao K, Okubo Y, Yasoda A, Koyama N, Osawa K, Isobe Y, Kondo E, Fujii T, Miura M, Nakao K, Bessho K
  • Journal Title: J Dent Res Volume: 92(1) Issue: 1 Pages: 58-64
  • DOI: 10.1177/0022034512466413
  • Peer Reviewed
• [Journal Article] Mastication and risk for diabetes in a Japanese population: a cross-sectional study. (2013)
  • Author(s): Yamazaki T, Yamori M, Asai K, Nakano-Araki I, Yamaguchi A, Takahashi K, Sekine A, Matsuda F, Kosugi S, Nakayama T, Inagaki N, Bessho K; Nagahama Study Collaboration Group.
  • Journal Title: PLoS One. Volume: 8 Issue: 6 Pages: e64113-e64113
  • DOI: 10.1371/journal.pone.0064113
  • Peer Reviewed
• [Journal Article] Phenotypes of CCAAT/enhancer-binding protein beta deficiency: Hyperdontia and elongated coronoid process. (2013)
  • Author(s): Huang, B., Takahashi, K., Sakata-Goto, T., Kiso, H., Togo, Y., Saito, K., Tsukamoto, H., Sugai, M., Akira, S., Shimizu, A. and Bessho, K.
  • Journal Title: Oral Diseases Volume: 19 Issue: 2 Pages: 144-150
  • DOI: 10.1111/j.1601-0825.2012.01963.x
  • Peer Reviewed
• [Journal Article] Assessing anteroposterior basal bone discrepancy with the Dental Aesthetic Index (2013)
  • Author(s): Huang B, Takahashi K, Yamazaki, T., Saito, K., Yamori, M.,Asai, K., Yoshikawa Y., Kamioka H.,, Yamashiro T., Bessho K
  • Journal Title: Angle Orthod Volume: 83 Issue: 3 Pages: 527-532
  • DOI: 10.2319/050312-369.1
  • Peer Reviewed
• [Journal Article] A pragmatic method for electronic medical record-based observational studies: devel oping an electronic medical records retrieval system for clinical research. (2012)
  • Author(s): Yamamoto K, Sumi E, Yamazaki T, Asai K, Yamori M, Teramukai S, Bessho K, Yokode M, Fukushima M.
  • Journal Title: BMJ Open Volume: 2 Issue: 6 Pages: e001622-e001622
  • DOI: 10.1136/bmjopen-2012-001622
  • NAID: 120005122347
  • Peer Reviewed
• [Presentation] CNP/GC-B 系は局所因子として内軟骨骨化を促進する (2014)
  • Author(s): 中 尾 一 祐, 八十田 明 宏, 大 澤 賢 次, 山 中 茂 樹, 小 山 典 昭
  • Organizer: 第32 回骨代謝学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-07-24 – 2014-07-26
• [Presentation] ヒトiPS細胞を用いて間葉系前駆細胞を介した骨芽細胞への分化誘導 (2013)
  • Author(s): 磯部悠、小山典昭、池野正幸、中尾一祐、高橋克、別所和久
  • Organizer: 第５8回 日本口腔外科学会総会
  • Place of Presentation: 福岡
• [Presentation] ヒトiPS細胞は間葉系前駆細胞を介して軟骨細胞へ分化する (2013)
  • Author(s): 小山 典昭, 三浦 晶子, 中尾 一祐, 上田 依利子, 山下 唯, 近藤 絵里, 藤井 寿人, 田浦 大輔, 金本 巨哲, 曽根 正勝, 八十田 明宏, 荒井 宏司, 別所 和久, 中尾 一和
  • Organizer: 2013年国際骨代謝学会・日本骨代謝学会 第2回合同国際会議
  • Place of Presentation: 神戸
• [Book] Chapter 5. Feasibility of Molecularly Targeted Therapy for Tooth Regeneration. In. Hideharu Hibi, Minoru Ueda, ed.: New Trends in Tissue Engineering and Regenerative Medicine- Official Book of the Japanese Society of Regenerative Medicine (2014)
  • Author(s): 2.Katsu Takahashi, Honoka Kiso, Kazuyuki Saito, Yumiko Togo, Hiroko Tsukamoto, Boyen Huang, Kazuhisa Bessho
  • Total Pages: 11
  • Publisher: InTech
• [Remarks] 京都大学医学部附属病院歯科口腔外科
  • URL: http://kuhp.kyoto-u.ac.jp/~oral_surg/Oral_Surg_index.html
• [Remarks] 京都大学大学院医学研究科・医学部
  • URL: http://www.med.kyoto-u.ac.jp/organization-staff/research/doctoral_course/r-057/