| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Recently, it is known that miRNAs exist and circulate in many types of body fluids. These reports suggest the possibility that circulating miRNA in body fluid becomes a novel biomarker for cancer patients. In this study, we have attempted to identify novel miRNA markers in saliva and serum for oral squamous cell carcinoma (OSCC) patients. We extracted and purified circulating miRNA in saliva and serum of OSCC patients and healthy volunteers, and then carried out miRNA microarray analysis and quantitative RT-PCR. Ten miRNAs, let-7b/c, miR-17, miR-23a/b, miR-24, miR-25, miR-26a, miR-122a, miR-191 were significantly reduced in the serum of the OSCC patients. Furthermore, miR-26a significantly inhibited the growth of human OSCC cells. These results suggest that several miRNAs may be novel biomarkers for OSCC patients, and useful as a tool for preemptive medicine in OSCC.
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