Clinical application of salivary microRNA to preemptive medicine for oral cancer

• Project/Area Number: 25670861
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Ehime University
• Principal Investigator: HAMAKAWA Hiroyuki 愛媛大学, 医学(系)研究科(研究院), 教授 (20127905)
• Co-Investigator(Kenkyū-buntansha): NAKASHIRO Koichi 愛媛大学, 大学院医学系研究科, 准教授 (90314880)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 口腔癌 / microRNA / 先制医療

Outline of Final Research Achievements

Recently, it is known that miRNAs exist and circulate in many types of body fluids. These reports suggest the possibility that circulating miRNA in body fluid becomes a novel biomarker for cancer patients. In this study, we have attempted to identify novel miRNA markers in saliva and serum for oral squamous cell carcinoma (OSCC) patients. We extracted and purified circulating miRNA in saliva and serum of OSCC patients and healthy volunteers, and then carried out miRNA microarray analysis and quantitative RT-PCR. Ten miRNAs, let-7b/c, miR-17, miR-23a/b, miR-24, miR-25, miR-26a, miR-122a, miR-191 were significantly reduced in the serum of the OSCC patients. Furthermore, miR-26a significantly inhibited the growth of human OSCC cells. These results suggest that several miRNAs may be novel biomarkers for OSCC patients, and useful as a tool for preemptive medicine in OSCC.

Research Products

• [Journal Article] Prognostic significance of interleukin-8 and CD163-positive cell-infiltration in tumor tissues in patients with oral squamous cell carcinoma (2014)
  • Author(s): Fujita Y, Okamoto M, Goda H, Tano T, Nakashiro K, Sugita A, Fujita T, Koido S, Homma S, Kawakami Y, Hamakawa H.
  • Journal Title: PLoS ONE Volume: 9 Issue: 12 Pages: e110378-e110378
  • DOI: 10.1371/journal.pone.0110378
  • Peer Reviewed / Open Access
• [Journal Article] Immuno-Chemoradiotherapy for patients with oral squamous cell carcinoma: augmentation of OK-432-induced helper T-cell 1 response by 5-FU and X-ray irradiation (2013)
  • Author(s): Tano T, Okamoto M, Kan S, Bando T, Goda H, Nakashiro K, Shimodaira S, Koido S, Honma S, Fujita T, Sato M, Yamashita N, Hamakawa H, Kawakami Y
  • Journal Title: Neoplasia Volume: 15 Pages: 805-814
  • Peer Reviewed