Delivery of bone-marrow stem cells and functional microRNA for atrophic salivary gland regeneration
| Project/Area Number |
25670864
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
SUMITA Yoshinori 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (50456654)
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| Co-Investigator(Kenkyū-buntansha) |
ASAHINA Izumi 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (30221039)
KAGAMI Hideaki 松本歯科大学, 歯学部, 教授 (80242866)
縣 秀樹 長崎大学, 医歯薬学総合研究科(歯学系), 客員研究員 (20581177)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 萎縮唾液腺 / 細胞治療 / microRNA / 唾液腺再生 / miRNA |
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| Outline of Final Research Achievements |
The specific hypothesis of this study is that bone marrow-derived endothelial progenitor cells (CD34-positive cells) can rescue the radiogenic salivary-gland atrophy. Furthermore, we assumed that the delivery of functional miRNA that contributes the proliferation or differentiation of salivary-gland epithelial cells may be able to enhance the effect of CD34-positive cell therapy. As a result, local administration of CD34-positive cells to submandibular glands could increase the salivary output of mice after 12Gy of head and neck irradiation. Currently, we are analyzing in vivo behavior of exogenous CD34-positive cells in detail, and administrating miRNAs with CD34-positive cells to irradiated mice.
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Report
(3 results)
Research Products
(3 results)
