| Project/Area Number |
25670868
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | National Cancer Center Japan |
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Principal Investigator |
HONDA Kazufumi 独立行政法人国立がん研究センター, 研究所, ユニット長 (10260936)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Taisuke 国立研究開発法人国立がん研究センター中央病院, 病理科 (00296708)
野村 武史 東京歯科大学, オーラルメディシン・口腔外科学講座, 教授 (60328268)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 唾液腺がん / 組織マイクロアレイ / プロテオミクス / 仮想病理画像 / 定量抗体プロテオミクス / 抗体基盤プロテオミクス / 画像解析ソフトウエア / 国際研究者交流 / 国際情報交換 |
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| Outline of Final Research Achievements |
To identify the biomarker for decision of therapeutic strategy in salivary gland carcinoma, we made tissue microarray of the salivary gland carcinoma, and searched it by antibody-based proteomics. We identified overexpression of actiin-4 (ACTN4) that is associated with cancer metastasis in salivary gland carcinoma. To confirm the gene amplification of ACTN4, we investigated the copy number of ACTN4 by FISH analysis. FISH (fluorescence in situ hybridization) revealed that overall survival time of patients with gene amplification of ACTN4 was significantly shorter than it of patients with normal copy number. The multivariate Cox regression analysis revealed that gene amplification of ACTN4 remained as an independent prognostic factor. The gene amplification of ACTN4 is a potential prognostic biomarker for salivary gland carcinoma.
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