Epithelial mesenchymal interaction mediated by exosomes in tooth development

• Project/Area Number: 25670869
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: IWAMOTO Tsutomu 徳島大学, 大学院医歯薬学研究部, 教授 (90346916)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 歯原性上皮細胞 / エクソソーム / Cd9 / 歯の発生 / 上皮ー間葉の相互作用 / テトラスパニン

Outline of Final Research Achievements

The process of tooth development is involved in two kinds of cell types, dental epithelial cells and dental mesenchymal cells. Those cells interacted with each other, which is called epithelial-meshenchyamal interactions. However, its mechanisms sill unclear. Here we are trying to find the gene that regulate in this process. we found that Cd9, a major component of exosome, is highly expressed in tooth. It is suggested that Cd9 play a role in epithelial-meshencymal interaction in tooth development.

Research Products

• [Journal Article] Recruitment of mesenchymal stem cells by stromal cell-derived factor 1α in pulp cells from deciduous teeth (2015)
  • Author(s): Akazawa Y., Hasegawa T., Yoshimura Y., Chosa N., Asakawa T., Ueda K., Sugimoto A., Kitamura T., Nakagawa H., Ishisaki A., Iwamoto T.
  • Journal Title: International Journal of Molecular Medicine Volume: 36 Issue: 2 Pages: 442-448
  • DOI: 10.3892/ijmm.2015.2247
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Interaction between Fibronectin and β1 Integrin is Essential for Tooth Development. (2015)
  • Author(s): Saito, K., Fukumoto, E., Yamada, A., Yuasa, K., Yoshizaki, K., Iwamoto, T., Saito, M., Nakamura, T., and Fukumoto, S.
  • Journal Title: PLOS one Volume: 10(4) Issue: 4 Pages: 1-12
  • DOI: 10.1371/journal.pone.0121667
  • NAID: 120006872347
  • Peer Reviewed / Open Access
• [Journal Article] Dental findings and management in a child with hypomelanosis of Ito. (2014)
  • Author(s): Tomokazu Hasegawa, Yuki Akazawa, Takamasa Kitamura, Asuna Sugimoto, Kimiko Ueda, Tsutomu Iwamoto
  • Journal Title: Pediatric Dental Journal Volume: 24 Issue: 3 Pages: 173-177
  • DOI: 10.1016/j.pdj.2014.08.002
  • Peer Reviewed / Open Access
• [Journal Article] Pannexin 3 inhibits proliferation of osteoprogenitor cells by regulating Wnt and p21 signaling (2014)
  • Author(s): Ishikawa M, Iwamoto T, Fukumoto S, Yamada Y
  • Journal Title: J Biol Chem Volume: 289(5) Issue: 5 Pages: 2839-2851
  • DOI: 10.1074/jbc.m113.523241
  • Peer Reviewed
• [Journal Article] 象牙芽細胞の分化と細胞外基質 (2014)
  • Author(s): 岩本 勉
  • Journal Title: 四国歯学会雑誌 Volume: 26(2) Pages: 23-28
  • NAID: 120005832492
• [Journal Article] Application of a tooth-surface coating material to teeth with discolored crowns. (2013)
  • Author(s): Hashimura T, Yamada A, Iwamoto T, Arakaki M, Saito K, Fukumoto S.
  • Journal Title: PEDIATRIC DENTAL JOURNAL Volume: 23(1) Pages: 44-50
  • Peer Reviewed
• [Journal Article] Biological roles of gap junction proteins in cartilage and bone development. (2013)
  • Author(s): Iwamoto T, Ishikawa M, Ono M, Nakamura T,Fukumoto S, Yamada Y.
  • Journal Title: ,J Oral Biosci Volume: 55(1) Pages: 29-33
  • Peer Reviewed
• [Presentation] 象牙質修復に関わる間葉系幹細胞の乳歯歯髄細胞由来SDF-1αによる制御 (2015)
  • Author(s): 赤澤 友基, 長谷川 智一, 岩本 勉
  • Organizer: 第53回日本小児歯科学会大会
  • Place of Presentation: 広島国際会議場（広島県広島市）
  • Year and Date: 2015-05-21
• [Presentation] 乳歯歯根膜由来細胞のSDF-1αを介した歯周組織恒常性に関わる細胞間相互作用 (2015)
  • Author(s): 長谷川 智一, 赤澤 友基, 吉村 善隆, 帖佐 直幸, 浅川 剛吉, 杉本 明日菜, 北村 尚正, 上田(山口) 公子, 中川 弘, 白石 真紀, 石崎 明, 岩本 勉
  • Organizer: 第53回日本小児歯科学会大会
  • Place of Presentation: 広島国際会議場（広島県広島市）
  • Year and Date: 2015-05-21