Scleraxis-mediated regulation of mechanical responses in the periodontal ligament

• Project/Area Number: 25670871
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Hiroshima University
• Principal Investigator: SHUKUNAMI Chisa 広島大学, 大学院医歯薬保健学研究院, 教授 (60303905)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Teruko 東北大学, 歯学研究科(研究院), 教授 (00127250) ; TAKIMOTO Aki 京都大学, 健康長寿社会の総合医療開発ユニット, 特定助教 (00378902)
• Research Collaborator: YOSHIMOTO Yuki 広島大学, 大学院医歯薬保健学研究院, 特任助教 (40735304)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 歯学 / メカニカルストレス / 歯根膜 / Scleraxis / トランスジェニックマウス

Outline of Final Research Achievements

During experimental tooth movement, Scleraxis (Scx) and Osterix (Osx) were upregulated on the tension side of the periodontal ligament (PDL). Scx and Osx were upregulated via activation of TGF-beta and BMP signaling, respectively. Overexpression of Scx resulted in significant inhibition of mineralization in cultured PDL cells under the osteoinductive conditions. Conversely, knockdown of Scx significantly upregulated the expression of Osteocalcin, a marker for bone formation in osteoinduced PDL cells. Thus, Scx and Osx antagonistically regulate tensile-force responsive remodeling of the PDL and alveolar bone.

Research Products

• [Journal Article] Scleraxis and osterix antagonistically regulate tensile force-responsive remodeling of the periodontal ligament and alveolar bone. (2015)
  • Author(s): Takimoto A., Kawatsu M., Yoshimoto Y., Kawamoto T., Seiryu M., Takano-Yamamoto T., Hiraki Y., Shukunami C.
  • Journal Title: Development. Volume: 142(4) Issue: 4 Pages: 787-796
  • DOI: 10.1242/dev.116228
  • Peer Reviewed / Open Access
• [Presentation] 腱・靱帯付着部の形成機構 (2015)
  • Author(s): 宿南 知佐
  • Organizer: 第28回日本軟骨代謝学会
  • Place of Presentation: 東京都文京区 東京医科歯科大学 鈴木章夫記念講堂
  • Year and Date: 2015-03-07
  • Invited
• [Presentation] 歯周靭帯形成を解析するためのin vivoモデルの構築 (2014)
  • Author(s): 吉本由紀，宿南知佐
  • Organizer: 第56回歯科基礎医学会学術大会・総会
  • Place of Presentation: 福岡市 福岡国際会議場
  • Year and Date: 2014-09-27
• [Presentation] Molecular mechanisms regulating tendon and ligament formation (2014)
  • Author(s): Chisa Shukunami
  • Organizer: The 11th Bone Biology Forum
  • Place of Presentation: 裾野市 富士教育研究所
  • Year and Date: 2014-08-23
  • Invited
• [Presentation] 腱と骨を連結する分子機構 (2014)
  • Author(s): 宿南知佐
  • Organizer: 第32回日本骨代謝学会学術集会
  • Place of Presentation: 大阪市 大阪国際会議場
  • Year and Date: 2014-07-26
  • Invited
• [Presentation] Establishment of an in vivo model for analysis of periodontal ligament formation (2014)
  • Author(s): Yuki Sugimoto, Aki Takimoto, Yuji Hiraki, Chisa Shukunami
  • Organizer: 第47回広島大学歯学会総会
  • Place of Presentation: 広島市 広島大学歯学部大講義室
  • Year and Date: 2014-06-21
• [Presentation] 筋骨格系を連結する腱・靱帯形成の分子機構 (2014)
  • Author(s): 宿南 知佐
  • Organizer: 第27回骨代謝セミナー
  • Place of Presentation: 東京都文京区、ホテル椿山荘東京 アンフィシアター
  • Year and Date: 2014-06-06
  • Invited
• [Presentation] 歯根膜細胞における伸展刺激に応答したScleraxis発現調節メカニズムの解析
  • Author(s): 川津正慶，岩崎将也，清流正弘，池田悦子，滝本晶，宿南知佐，山本照子
  • Organizer: 第72回日本矯正歯科学会大会
  • Place of Presentation: 長野
• [Presentation] 腱・靱帯と硬組織の連結を制御する分子機構
  • Author(s): 宿南 知佐
  • Organizer: 第28回日本整形外科学会基礎学術集会
  • Place of Presentation: 千葉
  • Invited