| Project/Area Number |
25670872
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Orthodontics/Pediatric dentistry
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
NAKANO Kazuhiko 大阪大学, 歯学研究科(研究院), 教授 (00379083)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OKAWA Rena 大阪大学, 歯学部附属病院, 助教 (80437384)
NAKA Shuhei 大阪大学, 歯学研究科, 助教 (10589774)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | ヘリコバクター・ピロリ菌 / 口腔 / 感染経路 / 遺伝子配列 / PCR法 / 感染根管 / 定着 / 唾液 / ゲノム / ureA / 歯髄 / 線維芽細胞 / 分子生物学的手法 / 検出系 |
|---|
| Outline of Final Research Achievements |
Helicobacter pylori infection is believed to be acquired in early childhood, mainly via the oral cavity. However, the route of infection has yet to be elucidated. In the present study, we constructed a novel PCR system for the identification of H. pylori DNA based on complete genome information of 48 H. pylori strains registered in a database. Analysis of infected root canal specimens using this system revealed a detection rate of 15%, indicating the root canal as a possible reservoir for H. pylori.
|
