Mechanism of aggravation of periodontitis caused by periodontitis-related bacteria transmitted from companion animals

• Project/Area Number: 25670873
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Osaka University
• Principal Investigator: NOMURA Ryota 大阪大学, 歯学研究科(研究院), 准教授 (90437385)
• Co-Investigator(Kenkyū-buntansha): NAKANO Michiyo 岡山大学, 医歯(薬)学総合研究科, 教授 (30359848) ; ASAI Fumitoshi 麻布大学, 獣医学部, 教授 (00511677)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 歯周病原性細菌 / 伴侶動物 / Porphyromonas gulae / 犬 / FimA / 僧帽弁閉鎖不全症 / 線毛型 / 伝播 / PCR 法 / リコンビナントタンパク / カイコモデル / 歯周病

Outline of Final Research Achievements

Information regarding transmission of periodontitis-related bacterial species between dogs and their owners is limited. In the present study, we detected periodontitis-related species in both dogs and their owners including Porphyromonas gulae, known to be a major bacterial pathogen of periodontitis in dogs. P. gulae possesses approximately 41-kDa filamentous appendages (FimA), which have been classified into three major genotypes; A, B, and C. Our results showed that P. gulae with FimA genotype C was associated with mitral regurgitation in dogs.

Research Products

• [Journal Article] Measurement of oral bacterial counts in dogs by dielectrophoretic impedance (2015)
  • Author(s): Takahashi S, Kuratani M, Tanaka M, Ito T, Kanemaki N, Shirai M, Nomura R, Nakano K, Asai F
  • Journal Title: Fundamental Toxicological Sciences Volume: 未定
  • NAID: 130005083361
  • Peer Reviewed
• [Journal Article] Correlation of Age with Distribution of Periodontitis-Related Bacteria in Japanese Dogs (2013)
  • Author(s): Hirai N, Shirai M, Kato Y, et al.
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Issue: 7 Pages: 999-1001
  • DOI: 10.1292/jvms.13-0041
  • NAID: 130003362159
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Presentation] 犬とその飼い主における歯周病原細菌の伝播と接触度に関する検討 (2014)
  • Author(s): 吉田翔，山崎由衛，仲野和彦，仲周平，野村良太，仲野道代
  • Organizer: 第52回日本小児歯科学会大会
  • Place of Presentation: 品川区立総合区民会館（東京都，品川区）
  • Year and Date: 2014-05-16
• [Presentation] Distribution of periodontitis-related bacterial species in dogs in same facility (2013)
  • Author(s): Yamasaki Y, Nakano K, Nomura R, et al
  • Organizer: IADR-APR 2013
  • Place of Presentation: Bangkok, Thailand
• [Presentation] Clinical significance of Porphyromonas gulae FimA classification in dogs (2013)
  • Author(s): Yamasaki Y, Nomura R, Nakano K, et al
  • Organizer: The Third International Symposium of Medical-Dental-Pharmaceutical Education and Research in Okayama
  • Place of Presentation: 岡山