| Project/Area Number |
25670877
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
NONAKA Kazuaki 九州大学, 歯学研究科(研究院), 教授 (90128067)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAZA Haruyoshi 九州大学, 大学院歯学研究院, 講師 (30336151)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 乳歯幹細胞 / 小児歯科学 / ヒト乳歯由来幹細胞 |
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| Outline of Final Research Achievements |
We isolated mesenchymal stem cells from human exfoliated deciduous teeth (SHED). To examine the therapeutic efficacy of SHED on osteoporosis, SHED was intravenously transplanted into MRL/lpr mice which were the model for osteoporosis. Systemic transplantation of SHED recovered the reduction of bone density and structures in MRL/lpr mice by microCT analysis. Histological analysis showed SHED transplantation increased and decreased the number of osteoblasts and TRAP-positive cells, respectively, in the long bones. Gene expression analysis showed that the expression levels of Runx2, ALP, and Osteocalcin were significantly increased in the bones. In addition, SHED transplantation reduced the serum concentrations of soluble receptor activator for nuclear factor κB ligand (sRANKL)and C-terminal telopeptides of type I collagen (CTX), which were related with bone resorption. These results indicated systematic SHED transplantation promoted the bone formation and inhibited the bone resorption.
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