Raman imaging of osteoblastic mineralization
| Project/Area Number |
25670883
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Periodontology
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| Research Institution | Osaka University |
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Principal Investigator |
MURAKAMI Shinya 大阪大学, 歯学研究科(研究院), 教授 (70239490)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Yoshinori 大阪大学, 大学院工学研究科, 招聘教授 (20386634)
KITAGAKI Jirouta 大阪大学, 歯学部附属病院, 助教 (90570292)
TAKEDACHI Masahide 大阪大学, 大学院歯学研究科, 助教 (60452447)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ラマン分光法 / 歯根膜細胞 / 極微小石灰化ノジュール / 非侵襲性観察 |
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| Outline of Final Research Achievements |
In this study, we observed osteoblast differentiation utilizing time-lapse Raman imaging non-invasively. We established the methods to observe and visualize the hydroxyapatite (HA) on the differentiating mouse osteoblast cell line, KUSA-A1 by Raman imaging. This method demonstrated that Raman imaging visualized the HA spot more specifically than Alizarin red S staining. In addition, time-resolved Raman imaging analysis revealed that HA was triggered to generate in which beta-carotene had been detected. The osteoblastic differentiation in KUSA-A1 was tacked by Raman imaging non-invasively, thus these observations demonstrated for the first time by Raman imaging. And it was proven that Raman imaging was the powerful for non-invasive analysis of the molecules involved in the complicated process of osteoblast differentiation.
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Report
(3 results)
Research Products
(8 results)
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[Presentation] Raman Imaging of Osteoblastic Mineralization
Author(s)
Aya Hashimoto, Liang-da Chiu, Keigo Sawada, Tomohiko Ikeuchi, Katsumasa Fujita, Masahide Takedachi, Yoshinori Yamaguchi, Satoshi Kawata, Shinya Murakami, Eiichi Tamiya
Organizer
7th International Conference on Advanced Vibrational Spectroscopy
Place of Presentation
Kobe Convention Center, Japan
Related Report
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[Presentation] Time-lapse Raman imaging of osteoblastic mineralization
Author(s)
Aya Hashimoto, Liang-da Chiu, Keigo Sawada, Tomohiko Ikeuchi, Katsumasa Fujita, Masahide Takedachi, Yoshinori Yamaguchi, Satoshi Kawata, Shinya Murakami, Eiichi Tamiya
Organizer
Japan-Taiwan Bilateral Conference: Biomedical and Plasmonic Imaging
Place of Presentation
National University, Taiwan
Related Report
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