| Project/Area Number |
25670883
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Periodontology
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| Research Institution | Osaka University |
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Principal Investigator |
MURAKAMI Shinya 大阪大学, 歯学研究科(研究院), 教授 (70239490)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Yoshinori 大阪大学, 大学院工学研究科, 招聘教授 (20386634)
KITAGAKI Jirouta 大阪大学, 歯学部附属病院, 助教 (90570292)
TAKEDACHI Masahide 大阪大学, 大学院歯学研究科, 助教 (60452447)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ラマン分光法 / 歯根膜細胞 / 極微小石灰化ノジュール / 非侵襲性観察 |
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| Outline of Final Research Achievements |
In this study, we observed osteoblast differentiation utilizing time-lapse Raman imaging non-invasively. We established the methods to observe and visualize the hydroxyapatite (HA) on the differentiating mouse osteoblast cell line, KUSA-A1 by Raman imaging. This method demonstrated that Raman imaging visualized the HA spot more specifically than Alizarin red S staining. In addition, time-resolved Raman imaging analysis revealed that HA was triggered to generate in which beta-carotene had been detected. The osteoblastic differentiation in KUSA-A1 was tacked by Raman imaging non-invasively, thus these observations demonstrated for the first time by Raman imaging. And it was proven that Raman imaging was the powerful for non-invasive analysis of the molecules involved in the complicated process of osteoblast differentiation.
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