| Project/Area Number |
25670884
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Periodontology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Satoru 大阪大学, 歯学部附属病院, 講師 (40359849)
NOZAKI Takenori 大阪大学, 歯学研究科, 助教 (30263304)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 歯周病 / 老化 / 歯根膜細胞 / SASP |
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| Outline of Final Research Achievements |
Senescent human periodontal ligament (HPDL) cells were established from the primary culture of periodontal ligament cells by the induction of replicative exhausion in vitro system. Character of the senescent HPDL cells was confirmed by the enlarged cell shape and arrest of cell growth rate. Enhanced senescence associated beta-galactosidase activity, reactive oxygen species (ROS) activity, increased expressions level of p16, p53, and Rb were detected in the senescent HPDL cells. Senescent HPDL cells showed the senescent associated secretory protein (SASP) phenotype characterized by the robust production of IL-6, IL-8 and expression of GRO alpha, MMP1 and MMP9. Addition of conditioned medium from the senescent HPDL cells retarded the migration velocity for the HPDL cells. Senescent HPDL cells and SASP factors could exacerbate the chronic inflammation and destruction of aged periodontal tissues in elderly populations.
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