| Project/Area Number |
25670888
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Periodontology
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| Research Institution | National Center for Geriatrics and Gerontology |
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Principal Investigator |
MATSUSHITA Kenji 独立行政法人国立長寿医療研究センター, 口腔疾患研究部, 部長 (90253898)
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| Co-Investigator(Kenkyū-buntansha) |
HAGIWARA Makoto 国立長寿医療研究センター, 口腔疾患研究部, 流動研究員 (30546099)
TADA Hiroyuki 東北大学, 大学院歯学研究科, 講師 (70431632)
磯田 竜太朗 独立行政法人国立長寿医療研究センター, その他部局等, その他 (40456942)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 酸化修飾 / 活性酸素 / Rab5 / ファゴサイトーシス / 歯周病 / 歯周病原細菌 / マクロファージ / 自然免疫 / s-ニトロシル化 / 細菌感染 / 低分子量Gタンパク質 / 肺炎 / 感染制御 / 一酸化窒素 |
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| Outline of Final Research Achievements |
It has been suggested that oxidatively modified proteins are associated with the pathogenesis of chronic inflammatory diseases and infectious diseases. However, little is known about how the oxidative modification of proteins contributes to the pathogenesis of periodontal diseases. In this study, we explored oxidatively modified proteins in mammalian cells by an inclusive analyzing technique and examined the role of oxidatively modified proteins in the pathogenesis of periodontal diseases. We examined oxidatively modified proteins in the macrophage cell line RAW264 cyclopaedically and found that a small G-protein, Rab5, was s-nitrosylated in the cells. S-nitrosylated Rab5 enhanced bacterial pagocytosis in RAW264 cells. S-nitrosylation of proteins may be an important mechanism to protect periodontal tissues from infection with periodontopathic bacteria.
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