Reversing Aging: Regeneration of Aged Tissues using Adult Stem Cells Inside a Tissue exposed to Hydrogen Sulfide

• Project/Area Number: 25670898
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Social dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: Yaegaki Ken 日本歯科大学, 生命歯学部, 教授 (40166468)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 再生医療 / 若返り / 膵臓 / 肝臓 / β細胞 / インスリン / 歯髄 / 幹細胞 / tooth / stem cell / insulin / pancreas / 硫化水素 / 骨髄幹細胞 / 乳歯幹細胞

Outline of Final Research Achievements

This study had shown that hydrogen sulfide increases the number of b-cells and also insulin secretion from the cells reproduced from human tooth pulp. However high glucose decreased the number of cells and insulin secretion because of apoptosis. Hence we revised the protocol of regeneration of b-cells from human tooth pulp, and increased hydrogen sulfide concentration to 10 times, and glucotoxicities were almost disappeared, and the cells produced large amount of insulin. Thus exposing human tissue to hydrogen sulfide may cause reversing aging of the pancreas.

Research Products

• [Journal Article] Regeneration of insulin-producing pancreatic cells using a volatile bioactive compound and human teeth (2014)
  • Author(s): Okada M, Imai T, Yaegaki K, Ishkitiev N, Tanaka T
  • Journal Title: J Breath Res. Volume: 8 Issue: 4 Pages: 046004-046004
  • DOI: 10.1088/1752-7155/8/4/046004
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Hydrogen sulphide increases hepatic differentiation of human tooth pulp stem cells compared with human bone marrow stem cells (2014)
  • Author(s): Okada M, Ishkitiev N, Yaegaki K, Imai T, Tanaka T, Fukuda M, Ono S, Haapasalo M
  • Journal Title: Int Endod J Volume: 47 Pages: 1142-1150
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Hydrogen sulfide increases hepatic differentiation of human tooth-pulp stem cells compared with human bone-marrow stem cells. (2014)
  • Author(s): Okada M, Ishkitiev N, Yaegaki K, Imai T, Tanaka T, Fukuda M, Haapasalo M.
  • Journal Title: Int Endod J. 2014 Feb 12. Volume: 47 Issue: 12 Pages: 1142-1150
  • DOI: 10.1111/iej.12262
  • Peer Reviewed
• [Presentation] The effect of H2S on pancreatic regeneration from human tooth pulp (2014)
  • Author(s): Okada M, Ishkitiev N, Yaegaki K, Imai T
  • Organizer: 第12回国際幹細胞研究学会
  • Place of Presentation: Vancouver (Canada)
  • Year and Date: 2014-06-18 – 2014-06-21
• [Presentation] 乳歯歯髄幹細胞の膵臓分化における 硫化水素の影響 (2014)
  • Author(s): 岡田 実緖, Ishkitiev N, 八重垣 健, 今井 敏夫
  • Organizer: 第63会日本口腔衛生学会総会
  • Place of Presentation: 熊本市民会館、（熊本県熊本市９
  • Year and Date: 2014-05-29 – 2014-05-31
• [Presentation] Pancreatic regeneration from human tooth pulp: effects of H2S (2014)
  • Author(s): Mio Okada, Nikolay Ishkitiev, Ken Yaegaki, Toshio Imai,
  • Organizer: AADR/CADR2014
  • Place of Presentation: Charlotte, USA
• [Presentation] Role of Breath Compounds (2014)
  • Author(s): Ken Yaegaki
  • Organizer: International Association of Breath Research
  • Place of Presentation: Torun、Poland