| Budget Amount *help |
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2013: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
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| Outline of Final Research Achievements |
Molecular dynamics in neuronal cells is essential for synaptic plasticity and subsequent modulation of cellular functions in a neuronal network. For realizing artificial control of living neuronal network, we demonstrate laser-induced perturbation into the neuronal dynamics in neurons. By fluorescence correlation spectroscopy of quantum-dot-conjugated AMPA-type glutamate receptors on neurons, the molecular motion was constrained in an optical trap. Moreover, femtosecond laser-induced stimulation was applied to evaluate neuronal activity in neuronal networks. Focusing a femtosecond laser into target neuron cultured on micro-electrode arrays, intracellular Ca2+ immediately increased at the laser spot and the spike signals were frequently observed at electrode which was close to the laser spot. These results suggest that propagation of neuronal activity was evoked by laser-induced stimulation.
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