Elucidation of dynamics in neuronal networks by focused laser-induced perturbation

• Project/Area Number: 25700031
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Life / Health / Medical informatics
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Hosokawa Chie 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (60435766)
• Project Period (FY): 2013-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000); Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2013: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
• Keywords: 神経細胞 / 光ピンセット / フェムト秒レーザー / ナノバイオ / 蛍光解析 / 神経科学 / 生物物理 / 細胞・組織

Outline of Final Research Achievements

Molecular dynamics in neuronal cells is essential for synaptic plasticity and subsequent modulation of cellular functions in a neuronal network. For realizing artificial control of living neuronal network, we demonstrate laser-induced perturbation into the neuronal dynamics in neurons. By fluorescence correlation spectroscopy of quantum-dot-conjugated AMPA-type glutamate receptors on neurons, the molecular motion was constrained in an optical trap. Moreover, femtosecond laser-induced stimulation was applied to evaluate neuronal activity in neuronal networks. Focusing a femtosecond laser into target neuron cultured on micro-electrode arrays, intracellular Ca2+ immediately increased at the laser spot and the spike signals were frequently observed at electrode which was close to the laser spot. These results suggest that propagation of neuronal activity was evoked by laser-induced stimulation.

Research Products

• [Journal Article] Effect of correlating adjacent neurons for identifying communications: Feasibility experiment in a cultured neuronal network (2018)
  • Author(s): Yoshi Nishitani, Chie Hosokawa, Yuko Mizuno-Matsumoto, Tomomitsu Miyoshi, Shinichi Tamura
  • Journal Title: AIMS Neuroscience Volume: 5 Issue: 1 Pages: 13-31
  • DOI: 10.3934/neuroscience.2018.1.18
  • Peer Reviewed / Open Access
• [Journal Article] Enhanced fluorescence microscopy with the Bull’s eye-plasmonic chip (2017)
  • Author(s): Tawa Keiko、Izumi Shota、Sasakawa Chisato、Hosokawa Chie、Toma Mana
  • Journal Title: Optics Express Volume: 25 Issue: 9 Pages: 10622-10622
  • DOI: 10.1364/oe.25.010622
  • Peer Reviewed / Open Access
• [Journal Article] 集光レーザービームの光摂動を用いた細胞機能操作技術の開発 (2017)
  • Author(s): 細川千絵
  • Journal Title: 化学工業 Volume: 68 Pages: 340-345
• [Journal Article] 集光フェムト秒レーザー刺激による神経回路網の応答特性 (2017)
  • Author(s): 細川 千絵, 藤岡 祐次, 工藤 卓, 田口 隆久
  • Journal Title: レーザー学会研究会報告 Volume: - Pages: 41-45
  • Peer Reviewed
• [Journal Article] Classification of spike wave propagations in a cultured neuronal network: Investigating a brain communication mechanism (2017)
  • Author(s): Y. Nishitani, C. Hosokawa, Y. Mizuno-Matsumoto, T. Miyoshi and S. Tamura
  • Journal Title: AIMS Neuroscience Volume: 4 Issue: 1 Pages: 1-13
  • DOI: 10.3934/neuroscience.2017.1.1
  • Peer Reviewed / Open Access
• [Journal Article] Surface plasmon-enhanced optical trapping of quantum-dot-conjugated surface molecules on neurons cultured on a plasmonic chip (2016)
  • Author(s): K. Miyauchi, K. Tawa, Suguru N. Kudoh, T. Taguchi and C. Hosokawa
  • Journal Title: Japanese Journal of Applied Physics Volume: 55 Issue: 6S1 Pages: 06GN04-06GN04
  • DOI: 10.7567/jjap.55.06gn04
  • NAID: 210000146677
  • Peer Reviewed / Open Access
• [Journal Article] 集光レーザー摂動による神経細胞ネットワークの局所操作 (2016)
  • Author(s): 細川 千絵
  • Journal Title: レーザー研究 Volume: 44 Pages: 244-249
  • NAID: 130007957200
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Spike code flow in cultured neuronal networks (2016)
  • Author(s): Shinichi Tamura, Yoshi Nishitani, Chie Hosokawa, Tomomitsu Miyoshi, Hajime Sawai, Takuya Kamimura, Yasushi Yagi, Yuko Mizuno-Matsumoto, and Yen-Wei Chen
  • Journal Title: Computational Intelligence and Neuroscience Volume: 2016 Pages: 1-11
  • DOI: 10.1155/2016/7267691
  • Peer Reviewed / Open Access
• [Journal Article] Simulation of code spectrum and code flow of cultured neuronal networks (2016)
  • Author(s): Shinichi Tamura, Yoshi Nishitani, Chie Hosokawa, Tomomitsu Miyoshi, and Hajime Sawai
  • Journal Title: Computational Intelligence and Neuroscience Volume: 2016 Pages: 1-12
  • DOI: 10.1155/2016/7186092
  • Peer Reviewed / Open Access
• [Journal Article] Multiplex communication by BP learning in neural network (2016)
  • Author(s): Shinichi Tamura, Yoshi Nishitani, Chie Hosokawa, Tomomitsu Miyoshi, Hajime Sawai, Yuko Mizuno-Matsumoto, and Yen-Wei Chen
  • Journal Title: Proceedings of 2016 9th International Congress on Image and Sign al Processing, BioMedical Engineering and Informatics (CISP-BMEI) Volume: 2016 Pages: 825-828
  • DOI: 10.1109/cisp-bmei.2016.7852824
  • Peer Reviewed
• [Journal Article] Feasibility of multiplex communication in a 2D mesh asynchronous neural network with fluctuations (2016)
  • Author(s): S. Tamura, Y. Nishitani and C. Hosokawa
  • Journal Title: AIMS Neuroscience Volume: 3 Issue: 4 Pages: 385-397
  • DOI: 10.3934/neuroscience.2016.4.385
  • Peer Reviewed / Open Access
• [Journal Article] 培養神経細胞表面に局在するグルタミン酸受容体タンパク質の光捕捉 (2016)
  • Author(s): 細川 千絵、岸本 龍典、前澤 安代、工藤 卓、田口 隆久
  • Journal Title: レーザー学会研究会報告 Volume: 16 Pages: 19-23
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Fluorescence microscopy imaging of cells with a plasmonic dish integrally molded (2016)
  • Author(s): K. Tawa, C. Sasakawa, T. Fujita, K. Kiyosue, C. Hosokawa, J. Nishii, M. Oike and N. Kakinuma
  • Journal Title: Japanese Journal of Applied Physics Volume: 55 Issue: 3S2 Pages: 03DF12-03DF12
  • DOI: 10.7567/jjap.55.03df12
  • NAID: 210000146215
  • Peer Reviewed
• [Journal Article] 集光レーザーを用いた神経細胞ネットワークの局所光操作 (2015)
  • Author(s): 細川 千絵
  • Journal Title: Molecular Electronics and Bioelectronics Volume: 26 Pages: 69-72
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Spike code and information flow in cultured neuronal networks and its simulation on 2D mesh network (2015)
  • Author(s): S. Tamura, Y. Nishitani, C. Hosokawa, Y. Mizuno-Matsumoto, C. Yen-Wei
  • Journal Title: Proceedings of the 2015 7th IEEE International Conference on Cybernetics and Intelligent Systems (CIS) and Robotics, Automation and Mechatronics (RAM) Pages: 191-195
  • DOI: 10.1109/iccis.2015.7274571
  • Peer Reviewed
• [Journal Article] Base of brain intelligence: Information flow in cultured neuronal networks and its simulation on 2D mesh network (2015)
  • Author(s): S. Tamura, Y. Nishitani, C. Hosokawa, Y. Mizuno-Matsumoto,
  • Journal Title: Proceedings of 2015 12th International Conference on Fuzzy Systems and Knowledge Discovery (FSKD) Pages: 1747-1752
  • DOI: 10.1109/fskd.2015.7382211
  • Peer Reviewed
• [Journal Article] Laser-induced perturbation into molecular dynamics localized in neuronal cell (2015)
  • Author(s): C. Hosokawa, N. Takeda, S. N. Kudoh, T. Taguchi
  • Journal Title: Proceedings of SPIE Volume: 9305 Pages: 93052N-93052N
  • DOI: 10.1117/12.2076348
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Trapping of Neural Cell Adhesion Molecules in Neurons with Resonant Optical Tweezers (2014)
  • Author(s): 武田尚子、工藤卓、田口隆久、細川千絵
  • Journal Title: IEEJ Transactions on Electronics, Information and Systems Volume: 134 Issue: 8 Pages: 1071-1077
  • DOI: 10.1541/ieejeiss.134.1071
  • NAID: 130004678013
  • ISSN: 0385-4221, 1348-8155
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] In Situ Sensitive Fluorescence Imaging of Neurons Cultured on a Plasmonic Dish Using Fluorescence Microscopy (2014)
  • Author(s): K. Tawa*, C. Yasui, C. Hosokawa, H. Aota and J. Nishii
  • Journal Title: ACS Appl. Mater. Interfaces Volume: 6 Issue: 22 Pages: 20010-20015
  • DOI: 10.1021/am505579u
  • Peer Reviewed
• [Journal Article] Optical Perturbation of Intracellular Molecular Dynamics of Single Neuron in Living Neuronal Network (2014)
  • Author(s): 細川千絵
  • Journal Title: Seibutsu Butsuri Volume: 54 Issue: 6 Pages: 325-326
  • DOI: 10.2142/biophys.54.325
  • NAID: 130004713036
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed
• [Journal Article] Synchronized Code Sequences from Spike Trains in Cultured Neuronal Networks (2014)
  • Author(s): Y. Nishitani, C. Hosokawa, Y. Mizuno-Matsumoto, T. Miyoshi, H. Sawai, S. Tamura
  • Journal Title: International Journal of Engineering and Industries Volume: 5 Pages: 12-24
  • Peer Reviewed
• [Journal Article] Multiplexed spatiotemporal communication model in artificial neural networks. (2014)
  • Author(s): Tamura, S., Nishitani, Y., Kamimura, T., Hosokawa, C., Miyoshi, T., Sawai, H., Mizuno-Matsumoyo, Y., CHen, Y-W.
  • Journal Title: Automation, Control and Intelligent Systems Volume: 1 Issue: 6 Pages: 121-130
  • DOI: 10.11648/j.acis.20130106.11
  • Peer Reviewed
• [Presentation] 光マニピュレーションを用いた細胞操作 (2018)
  • Author(s): 細川千絵
  • Organizer: 第111回テクノラボツアー「ナノ光マニピュレーションが拓く世界」
• [Presentation] フェムト秒レーザー照射による神経細胞の光刺激過程 (2018)
  • Author(s): 藤岡祐次、工藤卓、田口隆久、細川千絵
  • Organizer: 第65回応用物理学会春季学術講演会
• [Presentation] 光ピンセットを用いた神経細胞分子操作における細胞内電気活動計測 (2018)
  • Author(s): 岸本龍典、工藤卓、田口隆久、細川千絵
  • Organizer: 第65回応用物理学会春季学術講演会
• [Presentation] プラズモニックチップ上で培養した神経細胞表面AMPA受容体の光捕捉 (2018)
  • Author(s): 松林佑基、泉章太、田和圭子、 工藤卓、細川千絵
  • Organizer: 第65回応用物理学会春季学術講演会
• [Presentation] 光ピンセット集光時におけるAMPA受容体分子の動態観測 (2017)
  • Author(s): 岸本 龍典、前澤 安代、工藤 卓、田口 隆久、細川 千絵
  • Organizer: 第64回応用物理学会春季学術講演会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2017-03-14
• [Presentation] フェムト秒レーザー光刺激に伴う誘発応答の細胞外電位計測とCa2+イメージングの同時計測 (2017)
  • Author(s): 藤岡 祐次、中川 裕太、工藤 卓、田口 隆久、細川 千絵
  • Organizer: 第64回応用物理学会春季学術講演会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2017-03-14
• [Presentation] 集光レーザー摂動による神経回路網の局所操作 (2017)
  • Author(s): 細川 千絵
  • Organizer: 日本分光学会関西支部 平成28年度 講演会
  • Place of Presentation: 産総研関西センター（大阪府池田市）
  • Year and Date: 2017-03-03
  • Invited
• [Presentation] Molecular dynamics in an optical trap of glutamate receptors labeled with quantum-dots on living neurons (2017)
  • Author(s): T. Kishimoto, Y. Maezawa, S. N. Kudoh, T. Taguchi, C. Hosokawa
  • Organizer: The 4th Optical Manipulation Conference (OMC '17)
  • Int'l Joint Research
• [Presentation] 集光レーザー摂動を利用した神経回路の機能操作 (2017)
  • Author(s): 細川千絵
  • Organizer: 大阪大学フォトニクスセンター第52回フォトニクス・コロキアム
  • Invited
• [Presentation] 集光レーザービームの光摂動を利用した細胞機能操作手法の開発 (2017)
  • Author(s): 細川千絵
  • Organizer: 第20回光科学異分野横断セミナー
  • Invited
• [Presentation] Optical Trapping Dynamics of AMPA Receptors on Neurons Revealed by Fluorescence Analysis (2017)
  • Author(s): T. Kishimoto, Y. Maezawa, S. N. Kudoh, T. Taguchi, C. Hosokawa
  • Organizer: 9th International Conference on Molecular Electronics and Bioelectronics (M&BE9)
  • Int'l Joint Research
• [Presentation] Evoked Responses in Living Neuronal Networks with Femtosecond Laser-induced Stimulation (2017)
  • Author(s): Y. Fujioka, S. N. Kudoh, T. Taguchi, C. Hosokawa
  • Organizer: 9th International Conference on Molecular Electronics and Bioelectronics (M&BE9)
  • Int'l Joint Research
• [Presentation] Surface Plasmon Resonance based Optical Trapping of Neurotransmitter Receptors on Neurons cultured on a Plasmonic Chip (2017)
  • Author(s): Y. Matsubayashi, S. Izumi, K. Tawa, S. N. Kudoh, C. Hosokawa
  • Organizer: 9th International Conference on Molecular Electronics and Bioelectronics (M&BE9)
  • Int'l Joint Research
• [Presentation] Optical trapping dynamics of quantum-dot conjugated AMPA-type glutamate receptors on living neurons (2017)
  • Author(s): T. Kishimoto, Y. Maezawa, S. N. Kudoh, T. Taguchi, C. Hosokawa
  • Organizer: 2017 Hsinchu Summer Course and Workshop
  • Int'l Joint Research
• [Presentation] 集光レーザービームの光摂動を用いた培養神経回路網の局所操作 (2017)
  • Author(s): 細川千絵
  • Organizer: イメージング数理研究会2017夏
  • Invited
• [Presentation] プラズモニックチップを用いた神経細胞表面に局在するAMPA 受容体分子の光捕捉 (2017)
  • Author(s): 松林佑基、泉章太、田和圭子、 工藤卓、細川千絵
  • Organizer: 平成29年 電気学会 電子・情報・システム部門大会
• [Presentation] 培養日数に依存して変化するAMPA受容体分子の光捕捉過程 (2017)
  • Author(s): 岸本龍典、前澤安代、工藤卓、田口隆久、細川千絵
  • Organizer: 平成29年 電気学会 電子・情報・システム部門大会
• [Presentation] フェムト秒レーザー光刺激手法による神経回路網の誘発応答 (2017)
  • Author(s): 藤岡祐次、工藤卓、田口隆久、細川千絵
  • Organizer: 平成29年 電気学会 電子・情報・システム部門大会
• [Presentation] Bull’s Eyeプラズモニックチップによる培養神経回路網の膜電位蛍光イメージング (2017)
  • Author(s): 箕嶋渉、泉章太、細川千絵、工藤卓、田和圭子
  • Organizer: 第78回応用物理学会 秋季学術講演会
• [Presentation] Stimulation Mechanisms in Living Neuronal Cells with a Focused Femtosecond Laser (2017)
  • Author(s): 藤岡祐次、工藤卓、田口隆久、細川千絵
  • Organizer: 第55回日本生物物理学会年会
• [Presentation] 集光フェムト秒レーザー刺激による神経回路網の応答特性 (2017)
  • Author(s): 細川 千絵、藤岡 祐次、工藤 卓、田口 隆久
  • Organizer: 第6回 ニューロフォトニクス研究会
• [Presentation] Sensitive Voltage Sensitive Dye Imaging in Living Neuronal Network on Bull’s eye-Plasmonic Chips (2017)
  • Author(s): W. Minoshima, S. Izumi , C. Hosokawa, S. N. Kudoh, K. Tawa
  • Organizer: 30th International Microprocesses and Nanotechnology Conference (MNC 2017)
  • Int'l Joint Research
• [Presentation] 集光レーザービームの光摂動による神経細胞ネットワークの操作 (2017)
  • Author(s): 細川千絵
  • Organizer: 大阪大学生命機能研究科第175回研究交流会
  • Invited
• [Presentation] Optical trapping of quantum-dot-conjugated glutamate receptors on living neuronal cells (2017)
  • Author(s): C. Hosokawa, T. Kishimoto, Y. Maezawa, S. N. Kudoh, T. Taguchi
  • Organizer: International Symposium on Nanomedicine 2017 (ISNM2017)
  • Int'l Joint Research / Invited
• [Presentation] Sensitive Action Potential Imaging in Cultured Neuronal Network on the Plasmonic-chip (2017)
  • Author(s): W. Minoshima, S. Izumi , C. Hosokawa, S. N. Kudoh, K. Tawa
  • Organizer: International Symposium on Nanomedicine 2017 (ISNM2017)
  • Int'l Joint Research
• [Presentation] Molecular dynamics of glutamate-receptors in an optical trap on living neurons revealed by fluorescence correlation spectroscopy (2017)
  • Author(s): T. Kishimoto, Y. Maezawa, S. N. Kudoh, T. Taguchi, C. Hosokawa
  • Organizer: The first International Workshop by the 174th Committee JSPS “Symbiosis of Biology and Nanodevices
  • Int'l Joint Research
• [Presentation] Characterization of the surface plasmon-enhanced fluorescence and single-nanoparticle fluorescence imaging on the bull’s eye-plasmonic chip (2016)
  • Author(s): S. Izumi, C. Hosokawa, M. Toma, and K. Tawa
  • Organizer: 9th Asian and Oceanian Photochemistry Conference 2016 (APC2016)
  • Place of Presentation: Nanyang Technological University (Singapore)
  • Year and Date: 2016-12-04
  • Int'l Joint Research
• [Presentation] レーザー光ピンセットによる細胞操作 (2016)
  • Author(s): 細川 千絵
  • Organizer: 第36回ポリマー光部品(POC)研究会
  • Place of Presentation: 産総研関西センター（大阪府池田市）
  • Year and Date: 2016-11-30
  • Invited
• [Presentation] Spatiotemporal activity pattern in neuronal network evaluated by femtosecond laser induced stimulation (2016)
  • Author(s): 藤岡 祐次、中川 裕太、工藤 卓、田口 隆久、細川 千絵
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
  • Year and Date: 2016-11-25
• [Presentation] Laser-induced perturbation into molecular dynamics in neuronal network (2016)
  • Author(s): C. Hosokawa, Y. Fujioka, Y. Nakagawa, S. N. Kudoh, and T. Taguchi
  • Organizer: The 10th International Symposium on Nanomedicine (ISNM2016)
  • Place of Presentation: 産総研つくばセンター（茨城県つくば市）
  • Year and Date: 2016-11-24
  • Int'l Joint Research / Invited
• [Presentation] 培養神経細胞表面に局在するグルタミン酸受容体タンパク質の光捕捉 (2016)
  • Author(s): 細川 千絵、岸本 龍典、前澤 安代、工藤 卓、田口 隆久
  • Organizer: レーザー学会 第498回研究会 「ニューロフォトニクス」 （第5回 ニューロフォトニクス研究会）
  • Place of Presentation: 東京農工大学（東京都小金井市）
  • Year and Date: 2016-11-19
• [Presentation] Neuronal activity evoked by femtosecond laser-induced stimulation (2016)
  • Author(s): C. Hosokawa, Y. Nakagawa, S. N. Kudoh, and T. Taguchi
  • Organizer: 29th International Microprocesses and Nanotechnology Conference (MNC 2016)
  • Place of Presentation: ANA Crown Plaza Kyoto（京都府京都市）
  • Year and Date: 2016-11-08
  • Int'l Joint Research
• [Presentation] Optical trapping dynamics depend on initial assembling of quantum-dot- conjugated glutamate receptors on hippocampal neuron (2016)
  • Author(s): T. Kishimoto, Y. Maezawa, S. N. Kudoh, T. Taguchi, and C. Hosokawa
  • Organizer: 29th International Microprocesses and Nanotechnology Conference (MNC 2016)
  • Place of Presentation: ANA Crown Plaza Kyoto（京都府京都市）
  • Year and Date: 2016-11-08
  • Int'l Joint Research
• [Presentation] Optical trapping dynamics of AMPA-type glutamate receptors on hippocampal neurons (2016)
  • Author(s): 岸本 龍典、前澤 安代、工藤 卓、田口 隆久、細川 千絵
  • Organizer: ライフエンジニアリング部門シンポジウム2016（LE2016）
  • Place of Presentation: 大阪国際交流センター（大阪府大阪市）
  • Year and Date: 2016-11-03
• [Presentation] Multiplex communication by BP learning in neural network (2016)
  • Author(s): S. Tamura, Y. Nishitani, C. Hosokawa, T. Miyoshi, H. Sawai, Y. Mizuno-Matsumoto, and Y.-W. Chen
  • Organizer: 2016 9th International Congress on Image and Signal Processing, BioMedical Engineering and Informatics (CISP-BMEI)
  • Place of Presentation: Datong (China)
  • Year and Date: 2016-10-15
  • Int'l Joint Research
• [Presentation] 集光レーザー摂動による神経細胞ネットワークの局所操作技術の開発 (2016)
  • Author(s): 細川 千絵
  • Organizer: 第8 回 BioOpto Japan カンファレンス
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-09-15
  • Invited
• [Presentation] 顕微鏡の照射角制御によって最適化されたプラズモニックチップ上のナノ粒子の蛍光増強度 (2016)
  • Author(s): 泉 章太、細川 千絵、當麻 真奈、田和 圭子
  • Organizer: 2016年第77回応用物理学会秋季学術講演会
  • Place of Presentation: 朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2016-09-13
• [Presentation] Improvement of Single-nanoparticle Fluorescence Image on Bull's Eye-plasmonic Chip (2016)
  • Author(s): S. Izumi, C. Hosokawa, M. Toma, K. Tawa
  • Organizer: The 14th International Conference of Near-Field Optics, Nanophotonics and Related Techniques (NFO-14)
  • Place of Presentation: Act City Hamamatsu（静岡県浜松市）
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] Optical trapping and assembling dynamics of quantum-dot labeled AMPA receptors located on hippocampal neuron (2016)
  • Author(s): T. Kishimoto, Y. Maezawa, S. N. Kudoh, T. Taguchi, and C. Hosokawa
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-07-20
• [Presentation] 光ピンセットを用いた神経伝達物質受容体の分子動態制御 (2016)
  • Author(s): 岸本 龍典、前澤 安代、工藤 卓、田口 隆久、細川 千絵
  • Organizer: 応用物理学会関西支部平成28年度第1回講演会
  • Place of Presentation: 産総研関西センター（大阪府池田市）
  • Year and Date: 2016-06-17
• [Presentation] Optical trapping of quantum-dot conjugated cell surface molecules of neuronal cell cultured onto a plasmonic chip (2016)
  • Author(s): C. Hosokawa, K. Miyauchi, S. N. Kudoh, T. Taguchi, K. Tawa
  • Organizer: The 3rd Optical Manipulation Conference (OMC '16)
  • Place of Presentation: PacificoYokohama（神奈川県横浜市）
  • Year and Date: 2016-05-18
  • Int'l Joint Research
• [Presentation] Bull's Eye構造のプラズモニックチップを用いた蛍光標識ナノ粒子の蛍光顕微鏡観察 (2016)
  • Author(s): 泉章太、細川千絵、當麻真奈、田和圭子
  • Organizer: 2016年第63回応用物理学会春季学術講演会
  • Place of Presentation: 東京工業大学(東京都)
  • Year and Date: 2016-03-21
• [Presentation] 神経細胞表面のグルタミン酸受容体分子の初期集合状態に依存した光捕捉過程 (2016)
  • Author(s): 前澤安代、岸本龍典、工藤卓、田口隆久、細川千絵
  • Organizer: 2016年第63回応用物理学会春季学術講演会
  • Place of Presentation: 東京工業大学(東京都)
  • Year and Date: 2016-03-21
• [Presentation] 集光フェムト秒レーザーを用いた単一細胞刺激による神経細胞ネットワークの活動特性 (2016)
  • Author(s): 中川裕太、工藤卓、田口隆久、細川千絵
  • Organizer: 2016年第63回応用物理学会春季学術講演会
  • Place of Presentation: 東京工業大学(東京都)
  • Year and Date: 2016-03-21
• [Presentation] Fluorescence imaging of a single nanoparticle on the plasmonic chip (2015)
  • Author(s): K. Tawa, C. Sasakawa, C. Hosokawa
  • Organizer: Pacifichem 2015
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2015-12-18
  • Int'l Joint Research
• [Presentation] Enhanced optical trapping toward direct manipulation of cell surface molecules on neurons (2015)
  • Author(s): C. Hosokawa
  • Organizer: The 9th International Symposium on Nanomedicine (ISNM2015)
  • Place of Presentation: Mie, Japan
  • Year and Date: 2015-12-11
  • Int'l Joint Research / Invited
• [Presentation] 集光レーザー摂動による神経細胞ネットワークの局所操作 (2015)
  • Author(s): 細川千絵
  • Organizer: レーザー学会 第4回ニューロフォトニクス研究会
  • Place of Presentation: ホテルグランドヒル市ヶ谷(東京都)
  • Year and Date: 2015-12-04
• [Presentation] Plasmon-enhanced optical trapping of nanoparticles on a plasmonic chip (2015)
  • Author(s): K. Miyauchi, K. Tawa, S. N. Kudoh, T. Taguchi, C. Hosokawa
  • Organizer: The 28th International Microprocesses and Nanotechnology Conference (MNC2015)
  • Place of Presentation: Toyama, Japan
  • Year and Date: 2015-11-12
  • Int'l Joint Research
• [Presentation] Resonance laser effect on optical trapping of cell adhesion molecules on neurons (2015)
  • Author(s): C. Hosokawa, N. Takeda, S. N. Kudoh, T. Taguchi
  • Organizer: The 28th International Microprocesses and Nanotechnology Conference (MNC2015)
  • Place of Presentation: Toyama, Japan
  • Year and Date: 2015-11-12
  • Int'l Joint Research
• [Presentation] プラズモニックチップを用いた単一ナノ粒子の光捕捉過程の蛍光解析 (2015)
  • Author(s): 宮内康平、田和圭子、工藤卓、田口隆久、細川千絵
  • Organizer: 2015年第76回応用物理学会秋季学術講演会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2015-09-15
• [Presentation] Direct manipulation of molecular dynamics in neuronal network with laser-induced perturbation (2015)
  • Author(s): 細川千絵
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢大学(石川県)
  • Year and Date: 2015-09-15
  • Invited
• [Presentation] Functional analysis of living neuronal networks with a femtosecond laser-induced stimulation (2015)
  • Author(s): 中川裕太、工藤卓、田口隆久、細川千絵
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢大学(石川県)
  • Year and Date: 2015-09-14
• [Presentation] Sensitive Single-Particle Fluorescence Imaging with a Plasmonic Chip (2015)
  • Author(s): 田和圭子、笹川知里、細川千絵
  • Organizer: 2015年第76回応用物理学会秋季学術講演会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2015-09-14
• [Presentation] Optical Trapping Dynamics of Glutamate Receptors in Cultured Neurons (2015)
  • Author(s): 前澤安代、工藤卓、田口隆久、細川千絵
  • Organizer: 2015年第76回応用物理学会秋季学術講演会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2015-09-13
• [Presentation] Optical manipulation of cell surface molecles on neurons with surface plasmon resonance-based optical tweezers (2015)
  • Author(s): 宮内康平、田和圭子、工藤卓、田口隆久、細川千絵
  • Organizer: ライフエンジニアリング部門シンポジウム2015 (LE2015)
  • Place of Presentation: 九州工業大学(福岡県)
  • Year and Date: 2015-09-02
• [Presentation] Optical trapping of AMPA receptors labeled with quantum-dots on hippocampal neurons (2015)
  • Author(s): 細川千絵、前澤安代、工藤卓、田口隆久
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2015-07-29
• [Presentation] Optical trapping dynamics of neurotransmitter receptors on a neuronal cell (2015)
  • Author(s): Y. Maezawa, C. Hosokawa, S. N. Kudoh, T. Taguchi
  • Organizer: The 8th International Conference on Molecular Electronics and Bioelectronics (M&BE8)
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2015-06-23
  • Int'l Joint Research
• [Presentation] Fluorescence imaging of cells on the plasmonic dish integrally molded (2015)
  • Author(s): K. Tawa, T. Fujita, C. Sasakawa, K. Kiyosue, C. Hosokawa, J. Nishii, M. Oike, N. Kakinuma
  • Organizer: The 8th International Conference on Molecular Electronics and Bioelectronics (M&BE8)
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2015-06-22
  • Int'l Joint Research
• [Presentation] プラズモニックディッシュで培養した神経細胞の高感度蛍光イメージング (2015)
  • Author(s): 田和圭子、藤田剛、細川千絵、西井準治
  • Organizer: 第64回高分子学会年次大会
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2015-05-28
• [Presentation] 集光レーザーを用いた神経細胞ネットワークの局所光操作 (2015)
  • Author(s): 細川千絵
  • Organizer: 応用物理学会 有機分子・バイオエレクトロニクス分科会 研究会
  • Place of Presentation: 富山大学(富山県)
  • Year and Date: 2015-05-28
• [Presentation] Optical trapping and assembling of neurotransmitter receptor proteins localized on a neuronal cell (2015)
  • Author(s): C. Hosokawa, Y. Maezawa, N. Takeda, S. N. Kudoh, T. Taguchi
  • Organizer: The 2nd Optical Manipulation Conference (OMC15)
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2015-04-22
  • Int'l Joint Research / Invited
• [Presentation] プラズモニックチップ上で培養した神経細胞接着分子の光捕捉 (2015)
  • Author(s): 細川千絵、宮内康平、工藤卓、田口隆久、田和圭子
  • Organizer: 2015年第62回応用物理学会春季学術講演会
  • Place of Presentation: 東海大学(神奈川県)
  • Year and Date: 2015-03-13
• [Presentation] 光ピンセットを用いた神経細胞に局在する受容体タンパク質の捕捉・集合 (2015)
  • Author(s): 前澤安代、工藤卓、田口隆久、細川千絵
  • Organizer: 2015年第62回応用物理学会春季学術講演会
  • Place of Presentation: 東海大学(神奈川県)
  • Year and Date: 2015-03-11
• [Presentation] Root problem of image cognition : Spike code flow map in cultured neuronal networks (2015)
  • Author(s): S. Tamura, Y. Nishitani, C. Hosokawa, T. Miyoshi, H. Sawai, T. Kamimura, Y. Yagi, C. Hosokawa, Y. Mizuno-Matsumoto, T. Miyoshi, H. Sawai, C. Yen-Wei
  • Organizer: 電子情報通信学会 医用画像研究会
  • Place of Presentation: 石垣島ホテルミヤヒラ(沖縄県)
  • Year and Date: 2015-03-02
• [Presentation] SPIE Photonics West 2015 (2015)
  • Author(s): C. Hosokawa, N. Takeda, S. N. Kudoh, T. Taguchi
  • Organizer: Laser-induced perturbation into molecular dynamics localized in neuronal cell
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2015-02-07
  • Int'l Joint Research
• [Presentation] Optical control of single cell in a neuronal network by a focused laser beam (2014)
  • Author(s): C. Hosokawa, N. Takeda, Y. Nakagawa, S. N. Kudoh, T. Taguchi
  • Organizer: The 8th International Symposium on Nanomedicine (ISNM2014)
  • Place of Presentation: Matsuyama, Japan
  • Year and Date: 2014-12-06
  • Int'l Joint Research / Invited
• [Presentation] Optical perturbation into neuronal cells by a focused laser beam (2014)
  • Author(s): C. Hosokawa, N. Takeda, S. N. Kudoh, T. Taguchi
  • Organizer: The 27th International Microprocesses and Nanotechnology Conference (MNC2014)
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2014-11-06
  • Int'l Joint Research
• [Presentation] Surface plasmon-enhanced optical tweezers of cell surface molecules on neurons (2014)
  • Author(s): K. Miyauchi, K. Tawa, S. N. Kudoh, T. Taguchi, C. Hosokawa
  • Organizer: The 27th International Microprocesses and Nanotechnology Conference (MNC2014)
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2014-11-06
  • Int'l Joint Research
• [Presentation] High-contrast fluorescence imaging on a plasmonic chip with the transmitted light fluorescence microscope (2014)
  • Author(s): T. Fujita, C. Hosokawa, J. Nishii, N. Kakinuma, M. Oike, K. Tawa
  • Organizer: The 27th International Microprocesses and Nanotechnology Conference (MNC2014)
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2014-11-06
  • Int'l Joint Research
• [Presentation] Laser-induced perturbation into living neuronal networks: Toward understanding neurodynamics (2014)
  • Author(s): C. Hosokawa
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2014-09-25
  • Invited
• [Presentation] フェムト秒レーザーを用いた単一神経細胞刺激による神経活動の伝搬 (2014)
  • Author(s): 中川裕太、工藤卓、田口隆久、細川千絵
  • Organizer: ライフエンジニアリング部門シンポジウム2014
  • Place of Presentation: 金沢大学(石川県)
  • Year and Date: 2014-09-18
• [Presentation] 神経細胞表面分子の局所操作に向けたプラズモン光ピンセット手法の開発 (2014)
  • Author(s): 宮内康平、田和圭子、工藤卓、田口隆久、細川千絵
  • Organizer: 平成26年電気学会電子・情報・システム部門大会
  • Place of Presentation: 島根大学(島根県)
  • Year and Date: 2014-09-05
• [Presentation] フェムト秒レーザー光刺激による神経細胞ネットワークの活動制御 (2014)
  • Author(s): 中川裕太、工藤卓、田口隆久、細川千絵
  • Organizer: 平成26年電気学会電子・情報・システム部門大会
  • Place of Presentation: 島根大学(島根県)
  • Year and Date: 2014-09-03
• [Presentation] Bright fluorescence imaging of cells cultured on the plasmonic dish (2014)
  • Author(s): K. Tawa, T. Fujita, C. Yasui, K. Kiyosue, C. Hosokawa, C. Sasagawa, H. Aota, N. Kakinuma, M. Oike, J. Nishii
  • Organizer: The 248th ACS National Meeting
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2014-08-12
  • Int'l Joint Research
• [Presentation] 一体成形加工したプラズモニックディッシュ上で培養した神経細胞の蛍光顕微鏡イメージング (2014)
  • Author(s): 藤田剛、細川千絵、西井準冶、柿沼憲宏、尾池真、田和圭子
  • Organizer: 第63回高分子学会年次大会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2014-05-29
• [Presentation] 表面プラズモン共鳴効果を利用した神経細胞接着分子の光捕捉 (2014)
  • Author(s): 宮内康平、田和圭子、工藤卓、田口隆久、細川千絵
  • Organizer: ナノ学会第12回大会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2014-05-23
• [Presentation] Optical manipulation of molecular dynamics in neurons (2014)
  • Author(s): C. Hosokawa, N. Takeda, S. N. Kudoh, T. Taguchi
  • Organizer: The 1st Optical Manipulation Conference (OMC14)
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2014-04-23
  • Int'l Joint Research / Invited
• [Presentation] 量子ドットで標識した神経細胞接着分子動態の光操作
  • Author(s): 武田尚子、工藤卓、田口隆久、細川千絵
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 京都国際会館(京都府)
• [Presentation] Laser manipulation of intracellular molecular dynamics in hippocampal neurons
  • Author(s): C. Hosokawa, Y. Ueda, N. Takeda, S. N. Kudoh, and T. Taguchi
  • Organizer: The 10th Conference on Lasers and Electro-Optics Pacific Rim (CLEO-PR & OECC/PS 2013)
  • Place of Presentation: Kyoto, Japan
• [Presentation] レーザー誘起分子集合制御による神経回路網の局所機能操作
  • Author(s): 細川千絵
  • Organizer: 大阪府立大学大学院工学研究科 第76回電子物理工学セミナー
  • Place of Presentation: 大阪府立大学(大阪府)
  • Invited
• [Presentation] Intracellular molecular dynamics in an optical trap studied by fluorescence correlation spectroscopy
  • Author(s): C. Hosokawa, N. Takeda, Y. Ueda, S. N. Kudoh, and T. Taguchi
  • Organizer: The 7th International Conference on Advanced Vibrational Spectroscopy
  • Place of Presentation: Kobe, Japan
• [Presentation] 共鳴レーザー照射による神経細胞接着分子の光捕捉
  • Author(s): 武田尚子、工藤卓、田口隆久、細川千絵
  • Organizer: 平成25年電気学会 電気・情報・システム部門大会
  • Place of Presentation: 北見工業大学(北海道)
• [Presentation] フェムト秒レーザー切断による神経回路網の機能的結合特性評価
  • Author(s): 久保勇人、工藤卓、田口隆久, 細川千絵
  • Organizer: ライフエンジニアリング部門シンポジウム2013
  • Place of Presentation: 慶應義塾大学(神奈川県)
• [Presentation] 神経細胞接着分子の光捕捉過程における共鳴レーザー照射効果
  • Author(s): 武田尚子、工藤卓、田口隆久、細川千絵
  • Organizer: 第74回応用物理学会秋季学術講演会
  • Place of Presentation: 同志社大学(京都府)
• [Presentation] 光集合制御による神経細胞内分子動態の解明
  • Author(s): 細川千絵
  • Organizer: 日本物理学会2013年秋季大会
  • Place of Presentation: 徳島大学(徳島県)
  • Invited
• [Presentation] Functional connectivity of living neuronal metwork modulated by femtosecond laser cutting of neurites
  • Author(s): H. Kubo, S. N. Kudoh, T. Taguchi, and C. Hosokawa
  • Organizer: The 12th International Conference on Laser Ablation (COLA 2013)
  • Place of Presentation: Ischia, Italy
• [Presentation] Optical perturbation of intracellular molecular dynamics of single neuron in living neuronal network
  • Author(s): C. Hosokawa, N. Takeda, Y. Ueda, S. N. Kudoh, and T. Taguchi
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 京都国際会館(京都府)
  • Invited
• [Presentation] Spatio-temporal dynamics of spontaneous activity in living neuronal network by femtosecond laser-induced cutting of neurites
  • Author(s): H. Kubo, S. N. Kudoh, T. Taguchi, and C. Hosokawa
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 京都国際会館(京都府)
• [Presentation] Laser manipulation of living neuronal network
  • Author(s): C. Hosokawa, N. Takeda, Y. Ueda, S. N. Kudoh, and T. Taguchi
  • Organizer: The 7th International Symposium on Nanomedicine (ISNM2013)
  • Place of Presentation: Kitakyushu, Japan
  • Invited
• [Presentation] 神経回路網を自在に操る―レーザー光を用いたアプローチ―
  • Author(s): 細川千絵
  • Organizer: 日本知能情報ファジィ学会 第15回ブレイン・コンピューティング研究部会研究会
  • Place of Presentation: 関西学院大学(兵庫県)
  • Invited
• [Presentation] Root problem of image cognition - Hardware in brain -
  • Author(s): 田村進一、西谷陽志、細川千絵、三好智満、澤井元、上村拓矢、八木康史、水野(松本)由子、陳延偉
  • Organizer: 電子情報通信学会 医用画像研究会
  • Place of Presentation: 沖縄ぶんかテンブス館(沖縄県)
• [Presentation] 光マニピュレーションの新しい生物科学応用
  • Author(s): 細川千絵
  • Organizer: 日本化学会 第94回春季年会
  • Place of Presentation: 名古屋大学(愛知県)
  • Invited
• [Presentation] 創薬スクリーニングに向けた細胞機能評価：集光レーザービームの光摂動を用いたアプローチ
  • Author(s): 細川千絵
  • Organizer: 日本化学会 第94回春季年会
  • Place of Presentation: 名古屋大学(愛知県)
  • Invited
• [Book] フェムト秒レーザー光による単一神経細胞刺激 (2014)
  • Author(s): 細川千絵
  • Publisher: Clinical Neuroscience
• [Remarks] Researchmap
  • URL: http://researchmap.jp/chiehosokawa
• [Remarks] 産業技術総合研究所バイオメディカル研究部門
  • URL: http://unit.aist.go.jp/bmd/