• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification and characterization of microorganisms involved in the carbon and energy cycles in the deep biosphere

Research Project

Project/Area Number 25701003
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Environmental dynamic analysis
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

HORI Tomoyuki  国立研究開発法人産業技術総合研究所, 環境管理研究部門, 主任研究員 (20509533)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥23,790,000 (Direct Cost: ¥18,300,000、Indirect Cost: ¥5,490,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Keywords環境分析 / 深海環境 / 地球化学 / 環境 / 微生物
Outline of Final Research Achievements

In this study, we focused on anaerobic acetate oxidizers involved in the reduction of crystalline iron(III) oxides and successfully obtained their enrichment cultures from deep subseafloor core sediments. High-throughput sequencing of 16S rRNA genes revealed that iron reducers affiliated within the phylum Firmicutes may play a pivotal role in the carbon and energy cycles in the deep biosphere. Further, in order to investigate the ecophysiological features of the iron reducers, we established the ultra-high-sensitivity stable isotope probing of 16S rRNA.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (22 results)

All 2016 2015 2014 2013 Other

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (16 results) (of which Invited: 4 results) Remarks (1 results)

  • [Journal Article] Isolation of microorganisms involved in reduction of crystalline iron (III) oxides in natural environments2015

    • Author(s)
      Hori T., Aoyagi T., Itoh, H., Narihiro T., Oikawa A., Suzuki K., Ogata A., Friedrich, M.W., Conrad R., Kamagata Y.
    • Journal Title

      Frontiers in Microbiology

      Volume: 6

    • DOI

      10.3389/fmicb.2015.00386

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dynamic transition of chemolithotrophic sulfur-oxidizing bacteria in response to amendment with nitrate in deposited marine sediments2015

    • Author(s)
      Aoyagi T, Kimura M, Yamada N, Navarro RR, Itoh H, Ogata A, Sakoda A, Katayama Y, Takasaki M and Hori T
    • Journal Title

      Front. Microbiol.

      Volume: 6

    • DOI

      10.3389/fmicb.2015.00426

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] High-Resolution Dynamics of Microbial Communities during Dissimilatory Selenate Reduction in Anoxic Soil2015

    • Author(s)
      Ronald R Navarro, Tomo Aoyagi, Makoto Kimura, Hideomi Itoh, Yuya Sato, Yoshitomo Kikuchi, Atsushi Ogata, Tomoyuki Hori
    • Journal Title

      Environmental science & technology

      Volume: 49 Issue: 13 Pages: 7684-7691

    • DOI

      10.1021/es505210p

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 未培養微生物の代謝活性を検出する超高感度安定同位体プローブ法の開発2015

    • Author(s)
      青柳 智、堀 知行
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 73 Pages: 225-227

    • NAID

      40020476288

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ultra-high-sensitivity stable-isotope probing of rRNA by high-throughput sequencing of isopycnic centrifugation gradients2015

    • Author(s)
      青柳 智、花田 智、伊藤 英臣、佐藤 由也、尾形 敦、Michael W. Friedrich、菊池 義智、堀 知行
    • Journal Title

      ENVIRONMENTAL MICROBIOLOGY REPORTS

      Volume: 7 Issue: 2 Pages: 282-287

    • DOI

      10.1111/1758-2229.12243

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 超高感度rRNA-Stable Isotope Probingによる未知環境微生物群の機能解析2016

    • Author(s)
      堀 知行、青柳 智、田中亮一、尾形 敦
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 酸性鉱山廃水処理システムにおける微生物群集構造の高解像度解析2016

    • Author(s)
      青柳 智、堀 知行、稲葉 知大、Navarro R. Ronald、愛澤秀信、尾形 敦
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] セレン汚染土壌を対象とした生物還元処理法の実効性評価2016

    • Author(s)
      堀 知行、青柳 智、Navarro R. Ronald、木村真人、伊藤英臣、佐藤由也、菊池義智、尾形 敦
    • Organizer
      第50回日本水環境学会年会
    • Place of Presentation
      アスティ徳島(徳島県・徳島市)
    • Year and Date
      2016-03-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] 海底由来堆積物の嫌気分解過程における微生物間相互作用の解明2016

    • Author(s)
      青柳 智、Navarro R. Ronald、眞弓大介、尾形 敦、片山葉子、高崎みつる、堀 知行
    • Organizer
      第50回日本水環境学会年会
    • Place of Presentation
      アスティ徳島(徳島県・徳島市)
    • Year and Date
      2016-03-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] 環境微生物の機能を活用した重金属汚染水処理の現状と展望2015

    • Author(s)
      堀 知行、尾形 敦
    • Organizer
      平成27年度資源・素材関係学協会合同秋季大会
    • Place of Presentation
      愛媛大学(愛媛県・松山市)
    • Year and Date
      2015-09-08
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] A progress toward the comprehensive understanding of the microbial ecosystem function - Ultra-high-sensitivity stable-isotope probing by high-throughput sequencing of rRNA transcript -2014

    • Author(s)
      堀 知行
    • Organizer
      2nd AIST-Tsinghua University Workshop on Advanced Technology for Waste Water Reclamation and Reuse
    • Place of Presentation
      産総研つくば西(茨城)
    • Year and Date
      2014-11-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 海洋性化学合成硫黄細菌を起点とする海底堆積物の嫌気分解過程2014

    • Author(s)
      青柳 智、木村真人、Navarro R. Ronald、伊藤 英臣、山田 奈海葉、尾形 敦、迫田章義、高崎みつる、片山 葉子、堀 知行
    • Organizer
      環境微生物系学会合同大会2014
    • Place of Presentation
      静岡大学工学部(静岡)
    • Year and Date
      2014-10-21 – 2014-10-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Geobacter sp. AOG5 株で見出された結晶性酸化鉄に対する高い還元活性2014

    • Author(s)
      青柳 智、佐藤 由也、花田 智、尾形 敦、鎌形 洋一、堀 知行
    • Organizer
      第66回日本生物工学会大会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 次世代シークエンサーの適用による超高感度rRNA-Stable Isotope Probing - 微生物生態系機能の実態解明に向けた技術進展 -2014

    • Author(s)
      堀 知行
    • Organizer
      第17 回日本水環境学会シンポジウム
    • Place of Presentation
      滋賀県立大学(滋賀)
    • Year and Date
      2014-09-08 – 2014-09-10
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Metabolic potential and community structure of anaerobically respiring microorganisms enriched from deep subseafloor sediments of the Shimokita Coalbed, Japan2014

    • Author(s)
      堀 知行、青柳 智、Navarro R. Ronald、佐藤 由也、伊藤 英臣、星野辰彦、諸野祐樹、稲垣史生、鎌形 洋一
    • Organizer
      ISME 15
    • Place of Presentation
      ソウル(韓国)
    • Year and Date
      2014-08-24 – 2014-08-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] High-throughput sequencing of isopycnic centrifugation gradients improves the sensitivity of rRNA-based stable isotope probing2014

    • Author(s)
      青柳 智、伊藤 英臣、佐藤 由也、尾形 敦、花田 智、菊池 義智、堀 知行
    • Organizer
      ISME 15
    • Place of Presentation
      ソウル(韓国)
    • Year and Date
      2014-08-24 – 2014-08-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 次世代シークエンサーの適応によるRNA-SIPの高感度化2014

    • Author(s)
      青柳 智, 佐藤由也, 伊藤英臣, 花田 智, 菊池義智, 堀 知行
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学(神奈川)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 結晶性酸化鉄を還元する新しい微生物の分離培養と生理学的特徴2013

    • Author(s)
      青柳智, 堀知行, 伊藤英臣, 尾形敦, 成廣隆, 花田智, 鎌形洋一
    • Organizer
      第29回日本微生物生態学会大会
    • Place of Presentation
      鹿児島大学(鹿児島)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 次世代シークエンサーの適用によるRNA-SIPの高感度化2013

    • Author(s)
      青柳智, 佐藤由也, 伊藤英臣, 多胡香奈子, 早津雅仁, 花田智, 菊池義智, 堀知行
    • Organizer
      第29回日本微生物生態学会大会
    • Place of Presentation
      鹿児島大学(鹿児島)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Enrichment, isolation and characterization of microorganisms involved in reduction of crystalline iron(III) oxides in anoxic soil environments2013

    • Author(s)
      堀知行, 青柳智, 成廣隆, 伊藤英臣, 尾形敦, 花田智, 鎌形洋一
    • Organizer
      2nd Thunen Symposium on Soil Metagenomics
    • Place of Presentation
      Thunen Institute(Braunschweig, Germany)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Enrichment, isolation and characterization of microorganisms involved in reduction of crystalline iron(III) oxides in anoxic soil environments2013

    • Author(s)
      堀知行
    • Organizer
      Microbial Ecophysiology group seminar
    • Place of Presentation
      Bremen university(Bremen, Germany)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Remarks] 産業技術総合研究所 環境微生物研究グループホームページ

    • URL

      https://unit.aist.go.jp/emri/114envmicrob/index.html

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi