| Budget Amount *help |
¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2014: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2013: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
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| Outline of Final Research Achievements |
In this study, denitrifying anaerobic methane oxidizing (DAMO) microorganisms was used for development of nitrogen removal system, which reduces the emmition of green house gases. To collect the basic knowhow on the novel nitrogen removal process, continuous flow reactor system and batch experiment were conducted. Enrichment of DAMO microorganisms was achieved by using continuous flow reactor systems from paddy filed soils. Clone libraries of 16S rRNA and pmoA gene were constructed using NC10 bacterial specific primer pairs to detect DAMO microorganisms in the reactor. The enriched microorganisms might be affected not only the types of electron acceptor but also it’s loading rate. In addition, cultivation method strongly affect the microbial community structures of DAMO cultures. Further, in the step of DAMO reaction using nitrate, nitrate reduction to nitrite could be the late limiting steps.
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