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Bacterial networking though membrane vesicles

Research Project

Project/Area Number 25701012
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Modeling and technologies for environmental conservation and remediation
Research InstitutionUniversity of Tsukuba

Principal Investigator

TOYOFUKU Masanori  筑波大学, 生命環境系, 助教 (30644827)

Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
Fiscal Year 2015: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2014: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2013: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Keywordsベシクル / 細菌間情報伝達 / 細胞間相互作用 / メンブランベシクル / 細菌間シグナル伝達 / 微生物 / 微生物間相互作用 / 細胞間シグナル伝達 / 応用微生物学 / ナノバイオ / 応用微生物 / 微生物間コミュニケーション / シグナル伝達
Outline of Final Research Achievements

Most bacteria release membrane vesicles (MVs) to the environment. These MVs are reported to have various functions that are important to the biological system, while they are not fully understood. During this project we have revealed a novel mechanism of how these MVs are released from the cell. Furthermore, we found that MVs can carry hydrophobic cell-cell communication signals (long chain AHLs), that will trigger the gene expression of the MV receiving cells. MVs showed some tendency to attach to the same species. Our data will allow us to understand the MV driven bacterial interactions and could also serve as a platform for nanotechnology.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (47 results)

All 2017 2016 2015 2014 2013 Other

All Int'l Joint Research (5 results) Journal Article (24 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 22 results,  Open Access: 6 results,  Acknowledgement Compliant: 4 results) Presentation (12 results) (of which Int'l Joint Research: 1 results,  Invited: 6 results) Book (4 results) Remarks (2 results)

  • [Int'l Joint Research] University of Zurich/ETH(スイス)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] HelmholtzZentrum(ドイツ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Zurich/ETH/Agaroscope(スイス)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] シドニー工科大学(オーストラリア)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] HelmholtzZentrum(ドイツ)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Membrane vesicle-mediated bacterial communication2017

    • Author(s)
      Toyofuku M, Morinaga K, Hashimoto Y, Uhl J, Shimamura H, Inaba H, Schmitt-Kopplin P, Eberl L, Nomura N
    • Journal Title

      ISME J.

      Volume: 印刷中 Issue: 6 Pages: 1504-1509

    • DOI

      10.1038/ismej.2017.13

    • NAID

      120007129099

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Biofilm formation by Paracoccus denitrificans requires a type I secretion system dependent adhesin BapA2017

    • Author(s)
      Yoshida K, Toyofuku M, Obana N, Nomura N
    • Journal Title

      FEMS Microbiol Lett.

      Volume: 364 Issue: 4

    • DOI

      10.1093/femsle/fnx029

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Influence of the alginate production on cell-to-cell communication in Pseudomonas aeruginosa PAO12017

    • Author(s)
      Yang J, Toyofuku M, Sakai R, Nomura N
    • Journal Title

      Environ Microbiol Rep.

      Volume: 印刷中 Issue: 3 Pages: 239-249

    • DOI

      10.1111/1758-2229.12521

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A Versatile and Rapidly Deployable Device to Enable Spatiotemporal Observations of the Sessile Microbes and Environmental Surfaces2017

    • Author(s)
      Kiyokawa T, Usuba R, Obana N, Yokokawa M, Toyofuku M, Suzuki H, Nomura N
    • Journal Title

      Microbes and Environments

      Volume: 32 Issue: 1 Pages: 88-91

    • DOI

      10.1264/jsme2.ME16161

    • NAID

      130005519591

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 微生物が能動的に産生するメンブランベシクルの生合成と機能2016

    • Author(s)
      尾花望, 黒沢正治,豊福雅典, 野村暢彦
    • Journal Title

      化学と生物

      Volume: 54 Pages: 812-819

    • NAID

      130006176422

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] メゾスコピック微生物学の夜明け2016

    • Author(s)
      豊福雅典,森永花菜, 野村暢彦
    • Journal Title

      環境バイオテクノロジー学会誌

      Volume: 16 Pages: 23-29

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細胞破裂を介した細菌のバイオフィルムとベシクル形成2016

    • Author(s)
      豊福雅典,野村暢彦
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 74 Pages: 420-421

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Explosive cell lysis is a novel mechanism for the biogenesis of membrane vesicles and biofilms2016

    • Author(s)
      Turnbull L, Toyofuku M, Hynen AL, Kurosawa M, Pessi G, Petty NK, Osvath, Carcamo-Oyarce G, Gloag ES, Shimoni R, Omasits U, Ito S, Yap X, Monahan LG, Cavaliere R, Ahrens CH, Charles IG, Nomura N, Eberl L and Whitchurch CB
    • Journal Title

      Nature Communications

      Volume: 7 Issue: 1

    • DOI

      10.1038/ncomms11220

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The <i>Pseudomonas</i> Quinolone Signal Inhibits Biofilm Development of <i>Streptococcus mutans</i>2015

    • Author(s)
      Inaba T, Oura H, Morinaga K, Toyofuku M, Nomura N
    • Journal Title

      Microbes and Environments

      Volume: 30 Issue: 2 Pages: 189-191

    • DOI

      10.1264/jsme2.ME14140

    • NAID

      130005077992

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bacterial membrane vesicles, an overlooked environmental colloid: Biology, environmental perspectives and applications.2015

    • Author(s)
      Masanori Toyofuku, Yosuke Tashiro, Yusuke Hasegawa, Masaharu Kurosawa, Nobuhiko Nomura
    • Journal Title

      Advance in Colloid and Interface Science

      Volume: 226 Pages: 65-77

    • DOI

      10.1016/j.cis.2015.08.013

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Environmental factors that shape biofilm formation.2015

    • Author(s)
      Toyofuku M, Inaba T, Kiyokawa T, Obana N, Yawata Y, Nomura N.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 80 Issue: 1 Pages: 7-12

    • DOI

      10.1080/09168451.2015.1058701

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Inhibition of Pseudomonas aeruginosa Swarming Motility by 1-Naphthol and Other Bicyclic Compounds Bearing Hydroxyl Groups2015

    • Author(s)
      Oura H, Tashiro Y, Toyofuku M, Ueda K, Kiyokawa T, Ito S, Takahashi Y, Lee S, Nojiri H, Nakajima-Kambe T, Uchiyama H, Futamata H and Nomura N.
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 81(8) Issue: 8 Pages: 2808-2818

    • DOI

      10.1128/aem.04220-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quorum Sensing Materials as New Ligand for Palladium Catalyzed Migita-Kosugi-Stille Polycondensation2014

    • Author(s)
      Wang, A., M. Toyofuku, N. Nomura and H. Goto
    • Journal Title

      International Letters of Chemistry, Physics and Astronomy

      Volume: 13 Pages: 62-70

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aerobic Respiratory Enzymes, Cbb3-type Cytochrome Oxidases, Impact the Anaerobic Life of Pseudomonas aeruginosa2014

    • Author(s)
      Hamada, M., M. Toyofuku, T. Miyano, and N. Nomura
    • Journal Title

      J. Bacteriol

      Volume: 196 Issue: 22 Pages: 3881-3889

    • DOI

      10.1128/jb.01978-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Drug Repositioning Screening Identified Pentetic Acid as a Potential Therapeutic Agent for Suppressing the Elastase-mediated Virulence of Pseudomonas aeruginosa2014

    • Author(s)
      Gi M., J. Jeong, K. Lee, K.M. Lee, M Toyofuku, D.E. Yong, S.S. Yoon and J.Y. Choi
    • Journal Title

      Antimicrob. Agents Chemother,

      Volume: 58 Issue: 12 Pages: 7205-7214

    • DOI

      10.1128/aac.03063-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Physiological levels of nitrate support anoxic growth by denitrification of Pseudomonas aeruginosa at growth rates reported in cystic fibrosis lungs and sputum2014

    • Author(s)
      Line, L., M. Alhede, M. Kolpen, M. Kuhl, O. Ciofu, T. Bjarnsholt, C. Moser, M. Toyofuku, N. Nomura, N. Hoiby and P. O. Jensen
    • Journal Title

      Front. Microbiol

      Volume: 5 Pages: 554-554

    • DOI

      10.3389/fmicb.2014.00554

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] cAMP Signaling Affects Irreversible Attachment During Biofilm Formation by <i>Pseudomonas aeruginosa</i> PAO12014

    • Author(s)
      Kaori Ono, Rie Oka, Masanori Toyofuku, Ayane Sakaguchi, Masakaze Hamada, Shiomi Yoshida and Nobuhiko Nomura
    • Journal Title

      Microbes and Environments

      Volume: 29 Issue: 1 Pages: 104-106

    • DOI

      10.1264/jsme2.ME13151

    • NAID

      130003390914

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Membrane vesicle formation is associated with pyocin production under denitrifying conditions in Pseudomonas aeruginosa PAO1.2014

    • Author(s)
      Toyofuku M, Zhou S, Sawada I, Takaya N, Uchiyama H, Nomura N
    • Journal Title

      Environ Microbiol.

      Volume: 印刷中 Issue: 9 Pages: 2927-2938

    • DOI

      10.1111/1462-2920.12260

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Carotenoid Accumulation Correlates with Aggregation and Biofilm Development in Rhodococcus sp. SD-742013

    • Author(s)
      Yi-Ting Zheng, Masanori Toyofuku, Nobuhiko Nomura, and Shinsuke Shigeto
    • Journal Title

      Analytical Chemistry

      Volume: 85 Issue: 15 Pages: 7295-7301

    • DOI

      10.1021/ac401188f

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional visualization of mixed species biofilm formation together with its substratum2013

    • Author(s)
      Inaba T, Ichihara T, Yawata Y, Toyofuku M, Uchiyama H, Nomura N
    • Journal Title

      Microbiol Immunol.

      Volume: 57 Issue: 8 Pages: 589-593

    • DOI

      10.1111/1348-0421.12064

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Impact of Anaerobiosis on Strain-Dependent Phenotypic Variations in <i>Pseudomonas aeruginosa</i>2013

    • Author(s)
      Hao Fang, Masanori Toyofuku, Tatsunori Kiyokawa, Akihiko Ichihashi, Kazuhiro Tateda, Nobuhiko Nomura.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 77 Issue: 8 Pages: 1747-1752

    • DOI

      10.1271/bbb.130309

    • NAID

      10031195827

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dechlorination of chloral hydrate is influenced by the biofilm adhesion protein LapA in Pseudomonas putida LF542013

    • Author(s)
      Wanjun Zhang, Huhe, Yuanbai Pan, Nobuhiko Nomura, Toshiaki Nakajima, Hiroo Uchiyama
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 79 (13) Issue: 13 Pages: 4166-4169

    • DOI

      10.1128/aem.00804-13

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 活性汚泥中の硝化菌に対する微生物細胞間シグナルの影響2013

    • Author(s)
      河嶋伊都子、小林祐子、豊福雅典、橋本庸平、稲葉英樹、内山裕夫、野村暢彦
    • Journal Title

      環境バイオテクノロジー学会誌

      Volume: 13 Pages: 135-139

    • NAID

      40020320853

    • Related Report
      2013 Annual Research Report
  • [Journal Article] バイオフィルム研究をめぐる最近の潮流2013

    • Author(s)
      豊福雅典、尾花望、田代陽介、八幡穣、野村暢彦
    • Journal Title

      感染症

      Volume: 43 Pages: 1-6

    • Related Report
      2013 Annual Research Report
  • [Presentation] 複合微生物系における細胞間シグナル伝達とその応用2016

    • Author(s)
      豊福雅典, 諸星知広,池田宰,野村暢彦
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター,北海道札幌市
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Membrane vesicle release through explosive cell lysis2016

    • Author(s)
      豊福雅典
    • Organizer
      第50回緑膿菌感染症研究会
    • Place of Presentation
      ヒルトン東京お台場,東京都港区
    • Year and Date
      2016-02-04
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Intercellular membrane trafficking in bacteria2015

    • Author(s)
      Toyofuku M
    • Organizer
      The International Symposium on Microbial Response, Adaptation, and Evolution in the Environment
    • Place of Presentation
      東京大学,東京都文京区
    • Year and Date
      2015-10-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 細菌が能動的に生産する膜小胞, メンブランベシクル2015

    • Author(s)
      豊福雅典, Leo Eberl, 野村暢彦
    • Organizer
      日本農芸化学会
    • Place of Presentation
      岡山大学, 岡山県岡山市
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Membrane vesicle trafficking of a long-chain acyl-homoserine lactone, C16-HSL2014

    • Author(s)
      M. Toyofuku, Y. Hashimoto, H. Inaba, N. nomura.
    • Organizer
      5th ASM Conference on Cell-Cell Communication in Bacteria
    • Place of Presentation
      San Antonio Marriott Rivercenter, USA(Texas)
    • Year and Date
      2014-10-18 – 2014-10-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] メンブランベシクルから見えてくる新たな微生物間コミュニケーション、バイオフィルム像2014

    • Author(s)
      豊福雅典、Leo Eberl、野村暢彦
    • Organizer
      生物工学会
    • Place of Presentation
      札幌コンベンションセンター, 北海道札幌市
    • Year and Date
      2014-09-09 – 2014-09-10
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 細菌間メンブラントラフィック2014

    • Author(s)
      豊福雅典、Leo Eberl、野村暢彦
    • Organizer
      細菌学若手コロッセウム
    • Place of Presentation
      ホテルニセコいこいの村,北海道虻田郡
    • Year and Date
      2014-08-06 – 2014-08-08
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] メンブランベシクルによる微生物細胞間シグナル物質の伝達2014

    • Author(s)
      豊福雅典、橋本庸平、稲葉英樹、野村暢彦
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      東京、明治大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] メンブランベシクルによる活性汚泥中での微生物細胞間シグナル物質の伝達2013

    • Author(s)
      豊福雅典、橋本庸平、稲葉英樹、野村暢彦
    • Organizer
      第29回日本微生物生態学会
    • Place of Presentation
      鹿児島、鹿児島大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Membrane vesicle production under denitrifying conditions is associated with pyocin production in Pseudomonas aeruginosa2013

    • Author(s)
      Masanori Toyofuku, Masaharu Kurosawa, Gabriella Pessi, Leo Eberl, Nobuhiko Nomura
    • Organizer
      Pseudomonas conference 2013
    • Place of Presentation
      スイス、ローザンヌ大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Nitric oxide induce membrane vesicle production under denitrifying conditions in Pseudomonas aeruginosa PAO12013

    • Author(s)
      Masanori Toyofuku, Nobuhiko Nomura
    • Organizer
      ICoN3
    • Place of Presentation
      東京、中央大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Environment dependent regulation of membrane vesicle production in Pseudomonas aeruginosa2013

    • Author(s)
      Masanori Toyofuku, Hiroo Uchiyama, Nobuhiko Nomura
    • Organizer
      ISEV2013
    • Place of Presentation
      アメリカ, Boston park plaza
    • Related Report
      2013 Annual Research Report
  • [Book] “Bacterial interactions” In: Encyclopedia of Biocolloid and Biointerface Sciences2016

    • Author(s)
      Masanori Toyofuku, Youske Tashiro, Inaba Tomohiro, Nobuhiko Nomura
    • Publisher
      Wiley
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
  • [Book] 環境と微生物の辞典2014

    • Author(s)
      豊福雅典、野村暢彦
    • Publisher
      朝倉書店
    • Related Report
      2014 Annual Research Report
  • [Book] Pseudomonas Vol. 72014

    • Author(s)
      Masanori Toyofuku, George A. O’Toole, Nobuhiko Nomura
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] “Biofilm as a multicellular bacterial system” In: Biodegradative Bacteira2014

    • Author(s)
      Masanori Toyofuku, Nobuhiko Nomura
    • Total Pages
      18
    • Publisher
      Springer
    • Related Report
      2013 Annual Research Report
  • [Remarks] 筑波大学 プレスリリース

    • URL

      http://www.tsukuba.ac.jp/attention-research/p201703061024.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] http://www.envr.tsukuba.ac.jp/~microbio/

    • Related Report
      2014 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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