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Seafood safety in globalized age of foods and advanced use of genetic information by epidemiological analysis of foodborne pathogen

Research Project

Project/Area Number 25702003
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Eating habits
Research InstitutionIshikawa Prefectural University (2014-2016)
Kyoto University (2013)

Principal Investigator

NAKAGUCHI Yoshitsugu  石川県立大学, 生物資源環境学部, 准教授 (70378967)

Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords食中毒 / 食中毒菌 / 輸入食品 / 魚介類 / 東南アジア / 腸炎ビブリオ / グローバル化 / 細菌汚染 / 遺伝子検査法 / 食の安全 / 安全性
Outline of Final Research Achievements

In globalized age of foods, food safety is one of the most important issues. In this study of the food safety, I focused on foodborne pathogen in seafood. I researched contamination by both Vibrio parahaemolyticus and its virulent strain on seafood in Southeast Asian countries.
The seafood has high frequency of the contamination by V. parahaemolyticus and its virulent strain in Thailand, Malaysia, Indonesia, and Vietnam. I concerned about epidemic of V. parahaemolyticus infection in these area. In addition, I developed multiplex PCR modified to detect both V. parahaemolyticus and its virulent strain.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (72 results)

All 2017 2016 2015 2014 2013 Other

All Int'l Joint Research (6 results) Journal Article (29 results) (of which Int'l Joint Research: 10 results,  Peer Reviewed: 29 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (35 results) (of which Int'l Joint Research: 2 results,  Invited: 10 results) Book (1 results) Funded Workshop (1 results)

  • [Int'l Joint Research] プリンスオブソンクラ大学(タイ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] プトラマレーシア大学(マレーシア)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] アンダラス大学(インドネシア)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] プリンスオブソンクラ大学(タイ)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] プトラマレーシア大学(マレーシア)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] アンダラス大学(インドネシア)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Evaluation of cloves (Syzygium aromaticum) against antibiotics resistant Vibrio parahaemolyticus on seafood2016

    • Author(s)
      Elexson, N., Rukayadi, Y., Nakaguchi, Y., Nishibuchi, M., Son, R.
    • Journal Title

      International Food Research Journal

      Volume: 22 Pages: 2229-2233

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Prevalence and antibiotic resistance of Salmonella Enteritidis and Salmonella Typhimurium in raw chicken meat at retail markets in Malaysia2016

    • Author(s)
      Thung, T. Y., Mahyudin, N. A., Basri, D.F., Wan Mohamed Radzi, C. W., Nakaguchi, Y., Nishibuchi, M., Son, R.
    • Journal Title

      Polutry Science

      Volume: 95 Issue: 8 Pages: 1888-1893

    • DOI

      10.3382/ps/pew144

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Impact of human Campylobacter infections in South-East Asia: the contribution of the poultry sector2016

    • Author(s)
      Premarathne, J. M., Satharasinghe, D. A., Huat J. T., Basri, D. F., Rukayadi, Y., Nakaguchi, Y., Nishibuchi, M., Son, R.
    • Journal Title

      Critical Reviews in Food Science and Nutrition

      Volume: 印刷中 Issue: 18 Pages: 3971-3986

    • DOI

      10.1080/10408398.2016.1266297

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Antibiotic Susceptibility profile of Vibrio parahaemolyticus isolated from shrimp in Selangor, Malaysia2016

    • Author(s)
      Saifedden, G., Farinazleen, G., Nor-Khaizura, A., Kayali, A.Y., Nakaguchi, Y., Nishibuchi, M. Son, R.
    • Journal Title

      International Food Research Journal

      Volume: 23 Pages: 2732-2736

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Immuno-magnetic isolation and characterization and genetic relationship of Escherichia coli O26 from raw meats, Hat yai city, Songkhla, Thailand2015

    • Author(s)
      Sirikaew, S., Rattanachuay, P., Nakaguchi, Y., Sukhumungoon, P.
    • Journal Title

      Southeast Asian Journal of Tropical Medicine and Public Health

      Volume: 46 Pages: 241-253

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Molecular characterization of Escherichia coli isolated from different food sources.2015

    • Author(s)
      Cheah, Y. K., Tay, L. W., Aida, A. A., Son, R., Nakaguchi, Y., Nishibuchi, M.
    • Journal Title

      International Food Research Journal.

      Volume: 22 Pages: 31-40

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Risk assessment of acquiring listeriosis from consumption of chicken offal in Selangor, Malaysia2015

    • Author(s)
      Kuan, C. H., Goh, S. G., Loo, Y. Y., Chang, W. S., Lye, Y. L., Puspanadan, S., Shahril, N., Tang, J. Y. H., Mahyudin, N. A., Nishibuchi, M., Nakaguchi, Y., Son, R.
    • Journal Title

      nternational Food Research Journal

      Volume: 22 Pages: 1711-1718

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Quantification and antibiotic susceptibility of Salmonella spp., Salmonella Enteritidis and Salmonella Typhimurium in raw vegetables (ulam)2015

    • Author(s)
      Najwa, M. S., Rukayadi, Y., Ubong, A., Loo, Y. Y., Chang, W. S., Lye, Y. L., Thung, T. Y, Aimi, S. A., Malcomn, T. T. H, Goh, S. G., Kuan, C. H., Nakaguchi, Y., Nishibuchi, M., Son, R
    • Journal Title

      International Food Research Journal

      Volume: 22 Pages: 1761-1769

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Improvement of the quantitation method for the tdh+ Vibrio parahaemolyticus in molluscan shellfish based on most-probablenumber,immunomagnetic separation, and loop-mediated isothermal amplification2015

    • Author(s)
      Escalante-Maldonado, O. R., Kayali,A. Y., Yamazaki, W.,Vuddhakul, Nakaguchi, Y., Nishibuchi, M.
    • Journal Title

      Frontiers in microbiology

      Volume: 6 Pages: 1-10

    • DOI

      10.3389/fmicb.2015.00270

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Characterisation of L. monocytogenes strains isolated from salad vegetables2015

    • Author(s)
      Ponniah, J., Robin, T., Son R., Cheah, Y. K., Ghazali, F. M., Nishibuchi, M., Nakaguchi, Y., Malakar, P.
    • Journal Title

      International Journal of Food Safety, Nutrition and Public Health

      Volume: 5 Pages: 137-150

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Detection and quantification of pathogenic Vibrio parahaemolyticus in shellfish by using multiplex PCR and loop-mediated isothermal amplification assay2015

    • Author(s)
      Malcolm , T. T. H., Y. K. Cheah, C. W. J. W. M. Radzi, F. A. Kasim, H. K. Kantilal, T. Y. H. John, J. Martinez-Urtaza, Y. Nakaguchi, M. Nishibuchi, R. Son
    • Journal Title

      Food Control.

      Volume: 47 Pages: 664-671

    • DOI

      10.1016/j.foodcont.2014.08.010

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effect of detergents as antibacterial agents on biofilm of antibiotics-resistant Vibrio parahaemolyticus isolate2014

    • Author(s)
      Elexson, N., L. Afsah-Hejri, Y. Rukayadi, P. Soopna, H.Y. Lee, T.C. Tuan Zainazor, M. Nor Ainy, Y. Nakaguchi, Nishibuchi Mitsuaki, R. Son
    • Journal Title

      Food control

      Volume: 35 Issue: 1 Pages: 378-385

    • DOI

      10.1016/j.foodcont.2013.07.020

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Consumption of raw oysters: a risk factor for Vibrio parahaemolyticus infection2014

    • Author(s)
      New, C. Y., Kantilal, H. K., Tan, M. T. H., Nakaguchi, Y., Nishibuchi, M., Son, R.
    • Journal Title

      International Food Research Journal

      Volume: 21(6) Pages: 2459-2472

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transmission of Listeria monocytogenes from raw chicken meat to cooked chicken meat through cutting boards2014

    • Author(s)
      Goh, S. G., Leili, A-H., Kuan, C. H., Loo, Y. Y., Lye, Y. L., Chang, W. S., Soopna, P., Najwa, M. S., Tang, J. Y. H., Yaya, R., Nishibuchi, M., Nakaguchi, Y., Son, R.
    • Journal Title

      Food control

      Volume: 37 Pages: 51-55

    • DOI

      10.1016/j.foodcont.2013.08.030

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biofilm assessment of Vibrio parahaemolyticus from seafood using Random Amplified Polymorphism DNA-PCR2014

    • Author(s)
      Elexson, N., Yaya, R., Nor, A. M., Kantilal, H. K., Ubong, A., Yoshitsugu, N., Nishibuchi, M. Son, R.
    • Journal Title

      International Food Research Journal

      Volume: 21(1) Pages: 59-65

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantification of enterophathogenic Escherichia coli from retailed meats2014

    • Author(s)
      Pannuch, M., Sirikaew, S., Nakaguchi, Y., Nishibuchi, M., Sukhumungoon, P.
    • Journal Title

      International Food Research Journal

      Volume: 21(2) Pages: 547-551

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization and relationship of methicillin-resistant Staphylococcus aureus among strains from healthy carriers and university hospital patients, southern Thailand2014

    • Author(s)
      Sukhumungoon P, Hayeebilan F, Yadrak P, Kanobthammakul S, Nakaguchi Y., Saengsuwan P, Singkhamanan K.
    • Journal Title

      Southeast Asian J Trop Med Public Health

      Volume: 45(2) Pages: 402-412

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] First report in Thailand of a stx-negative Escherichia coli O157 strain from a patient with diarrhea2014

    • Author(s)
      Themphachana, M., Nakaguchi, Y., Nishibuchi, M., Seto, K., Rattanachuay, P., Singkhamanan, K., Sukhumungoon, P.
    • Journal Title

      Southeast Asian Journal of Tropical Medicine and Public Health

      Volume: 45(4) Pages: 1-9

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Virulence characteristics and antimicrobial susceptibility of uropathogens from patients on Phuket island, Thailand2014

    • Author(s)
      Themphachana, M., Kanobthammakul, S., Nakaguchi, Y., Singkhamanan, K., Yadrak, P., Sukhumungoon, P.
    • Journal Title

      Southeast Asian Journal of Tropical Medicine and Public Health

      Volume: 45(5) Pages: 1-9

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Contamination by <i>Vibrio parahaemolyticus</i> and Its Virulent Strains in Seafood Marketed in Thailand, Vietnam, Malaysia, and Indonesia2013

    • Author(s)
      Yoshitsugu Nakaguchi
    • Journal Title

      Tropical Medicine and Health

      Volume: 41 Issue: 3 Pages: 95-102

    • DOI

      10.2149/tmh.2011-06

    • NAID

      130003383625

    • ISSN
      1348-8945, 1349-4147
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterisation of Vibrio parahaemolyticus carrying tdh and trh genes using ERIC-, RAPD- and BOX-PCR on local Malaysia bloody clam and Lala2013

    • Author(s)
      Yoke-Kqueen, C., Teck-Ee, K., Son, R., Yoshitsugu, N., Mitsuaki, N.
    • Journal Title

      International Food Research Journal

      Volume: 20(6) Pages: 3299-3305

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantification of Escherichia coli O157:H7 in organic vegetables and chickens2013

    • Author(s)
      Chang, W.S., Afsah-Hejri, L., Rukayadi, Y., Khatib, A., Lye, Y. L., Loo, Y. Y., Mohd Shahril, N., Puspanadan, S., Kuan, C.H., Goh, S.G., John, Y.H.T., Nakaguchi, Y., Nishibuchi, M. Son, R.
    • Journal Title

      International Food Research Journal

      Volume: 20(2) Pages: 1023-1029

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence and quantification of Listeria monocytogenes in chicken offal at the retail level in Malaysia2013

    • Author(s)
      C. H. Kuan, S. G. Goh, Y. Y. Loo, W. S. Chang, Y. L. Lye, S. Puspanadan, J. Y. H. Tang, Y. Nakaguchi, M. Nishibuchi, N. A. Mahyudin, S. Radu
    • Journal Title

      Polutry Science

      Volume: 92 Issue: 6 Pages: 1664-1669

    • DOI

      10.3382/ps.2012-02974

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficiency of four Malaysian commercial disinfectants on removing Listeria monocytogenes biofilm2013

    • Author(s)
      Fouladynezhad, N., Afsah-Hejri, L., Rukayadi, Y., Nakaguchi, Y., Nishibuchi, M. Son, R.
    • Journal Title

      International Food Research Journal

      Volume: 20(3) Pages: 1485-1490

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of extended-spectrum β -lactamases (ESBLs) producers in Klebsiella pneumoniae by genotypic and phenotypic method2013

    • Author(s)
      Puspanadan, S., Afsah-Hejri, L., John, Y.H.T., Rukayadi, Y., Loo, Y.Y, Nillian, E., Kuan, C.H., Goh, S.G., Chang, W.S., Lye, Y.L., Mohd Shahril, N., Nakaguchi, Y., Nishibuchi, M., Son, R.
    • Journal Title

      International food research Journal

      Volume: 20(3) Pages: 1479-1483

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fate of Thai Escherichia coli O157:H7 and Non-O157 lineages in Pasteurized Milk2013

    • Author(s)
      Thanaporn Pewleang, Yoshitsugu Nakaguchi, Pharanai Sukhumungoon
    • Journal Title

      Life Science Journal

      Volume: 10(4) Pages: 2368-2373

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Shiga Toxin 2-Converting Bacteriophages Occupy sbcB Gene as a Primary Integration Site in Bovine-Origined Escherichia coli O157:H7 and Non-O157 from Thailand2013

    • Author(s)
      Pharanai Sukhumungoon, Yoshitsugu Nakaguchi
    • Journal Title

      Life Science Journal

      Volume: 10(4) Pages: 2334-2340

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative detection and characterization of Shiga toxin-producing Escherichia coli O157 and non-O157 in raw vegetables by MPN-PCR in Malaysia2013

    • Author(s)
      Loo, Y. Y, Puspanadan, S., Goh, S. G., Kuan, C. H., Chang, W. S., Lye, Y. L., John, Y. H. T, Rukayadi, Y., Yoshitsugu, N.,Nishibuchi, M. Son, R.
    • Journal Title

      International Food Research Journal

      Volume: 20(6) Pages: 3313-3317

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Risk of Escherichia coli O157:H7 transmission linked to the consumption of raw milk

    • Author(s)
      Lye, Y. L., Afsah-Hejri, L., Chang, W. S., Loo, Y. Y., Puspanadan, S., Kuan, C. H., Goh, S. G., Shahril, N., Rukayadi, Y., Khatib, A., John, Y.H.T., Nishibuchi, M., Nakaguchi, Y. Son, R.
    • Journal Title

      International food research Journal

      Volume: 20(2) Pages: 1001-1005

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] グローバルからグローカルへ:東南アジアにみる人・食・感染症のダイナミズム2017

    • Author(s)
      中口義次
    • Organizer
      第48回日本食品微生物学会学術セミナー
    • Place of Presentation
      岡山コンベンションホール(岡山県)
    • Year and Date
      2017-12-19
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 天然精油の抗菌効果の探索と食品分野への利用の可能性2017

    • Author(s)
      中口義次
    • Organizer
      日本防菌防黴学会第44回年次大会
    • Place of Presentation
      千里ライフサイエンスセンター(大阪府)
    • Year and Date
      2017-09-26
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] アジア地域での食と感染症2017

    • Author(s)
      中口義次
    • Organizer
      BMSA北越地域拠点第一回セミナー「感染症の現状と予防」
    • Place of Presentation
      しいのき迎賓館(石川県)
    • Year and Date
      2017-01-19
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 寿司刺身を安全に食べるために:天然素材の食中毒菌に対する抗菌効果の探索2016

    • Author(s)
      澤田恵実、西渕光昭、中口義次
    • Organizer
      第50回腸炎ビブリオシンポジウム
    • Place of Presentation
      大阪大学微生物病研究所(大阪府)
    • Year and Date
      2016-10-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] 天然精油の食中毒菌に対する抗菌効果の検討2016

    • Author(s)
      澤田恵実、西渕光昭、中口義次
    • Organizer
      日本防菌防黴学会第43回年次大会
    • Place of Presentation
      品川区立総合区民会館「きゅりあん」(東京都)
    • Year and Date
      2016-09-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] 天然素材としての精油及びその揮発成分の抗菌効果の評価2016

    • Author(s)
      澤田恵実、西渕光昭、中口義次
    • Organizer
      第35回日本食品微生物学会学術総会
    • Place of Presentation
      タワーホール船堀(東京都)
    • Year and Date
      2016-09-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] Food safety and infectious disease in Asia2016

    • Author(s)
      Nakaguchi, Y.
    • Organizer
      International workshop: Detection of Enterohaemorrhagic E. coli on beef and fecal diarrheal samples with LAMP, IMS and PCR method
    • Place of Presentation
      Andalas University, Padang, Indonesia
    • Year and Date
      2016-09-13
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 攻めと守りの食中毒―石川で考えること―2016

    • Author(s)
      中口義次
    • Organizer
      平成28年度石川県立大学公開講座「県大から発信する食の安全・安心」
    • Place of Presentation
      野々市市情報交流会館カメリア(石川県)
    • Year and Date
      2016-05-28
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Frequent sequence variation of Vibrio parahaemolyticus trh gene affects considerably the clinical significance of the thermostable direct hemolysin-related hemolysin2016

    • Author(s)
      Escalante-Maldonado, O. R., Iwade, Y., Nakaguchi, Y., Nishibuchi, M.
    • Organizer
      50th Annual Joint Panel Meeting on Cholera & Other Bacterial Enteric Infections Panel Meeting, United States-Japan Cooperative Medical Science Program
    • Place of Presentation
      North Bethesda, Maryland, USA
    • Year and Date
      2016-01-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] trh gene sequence variation and its significance in the pathogenecity of Vibrio parahaemolyticus2015

    • Author(s)
      Escalante-Maldonado, O. R., Iwade, Y., Nakaguchi, Y., Nishibuchi M.
    • Organizer
      日本防菌防黴学会第42回年次大会
    • Place of Presentation
      千里ライフサイエンスセンター(大阪府豊中市)
    • Year and Date
      2015-09-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Improvement of IMS-LAMP-based Detection Method for Pathogenic Vibrio parahaemolyticus from Seafood2015

    • Author(s)
      Oscar R. Escalante Maldonado, Ahmad Yaman Kayali, 山崎 渉, 権平 文夫, 中口 義次, 西渕 光昭
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      長良川国際会議場(岐阜県岐阜市)
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Optimization of the LAMP-IMS-based method for detecting enterohemorrhagic Escherichia coli from beef2015

    • Author(s)
      Ahmad Yaman Kayali, Oscar Escalante Maldonado, 権平 文夫, 中口 義次, 西渕 光昭
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      長良川国際会議場(岐阜県岐阜市)
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] グローバル化がもたらす食と感染症―日本に、そして金沢にやってくる食、人、感染症―2014

    • Author(s)
      中口義次
    • Organizer
      第108回日本食品衛生学会学術講演会
    • Place of Presentation
      金沢歌劇座(石川県金沢市)
    • Year and Date
      2014-12-04 – 2014-12-06
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Prevalence of Shigatoxin-producing Escherichia coli belonging to enterohemorrhagic O serotypes (O157 and non-O157) in the beef marketed in southern Thailand in years 2013 and 20142014

    • Author(s)
      Ahmad Yaman Kayali, Oscar R. Escalante-Maldonado, Kensuke Ozawa, Fumio Gondaira, Varaporn Vuddhakul, Yoshitsugu Nakaguchi, and Mitsuaki Nishibuchi
    • Organizer
      第67回日本細菌学会関西支部総会
    • Place of Presentation
      兵庫医科大学西宮キャンパス(兵庫県西宮市)
    • Year and Date
      2014-11-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] Development of trh gene LAMP primers and establishment of IMS-LAMP based method for detection of pathogenic Vibrio parahaemolyticus in seafood2014

    • Author(s)
      Oscar R. Escalante-Maldonado, Ahmad Yaman Kayali, Sawa Yamada, Wataru Yamazaki, Fumio Gondaira, Varaporn Vuddhakul, Yoshitsugu Nakaguchi, and Mitsuaki Nishibuchi
    • Organizer
      第67回日本細菌学会関西支部総会
    • Place of Presentation
      兵庫医科大学西宮キャンパス(兵庫県西宮市)
    • Year and Date
      2014-11-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] Prevalence of pathogenic strains of Vibrio parahaemolyticus in popular shellfish (molluscan bivalves) marketed in southern Thailand in the 2014 study as demonstrated by a sensitive detection method2014

    • Author(s)
      Oscar R. Escalante-Maldonado、Ahmad Yaman Kayali、山田佐和、山崎渉、権平文夫、Varaporn Vuddhakul、中口義次、西渕光昭
    • Organizer
      第48回腸炎ビブリオシンポジウム
    • Place of Presentation
      (金森商船(株)函館ヒストリープラザ金森ホール(北海道函館市)
    • Year and Date
      2014-11-12 – 2014-11-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] グローバルからグローカルへ:アジアにみる食と感染症のダイナミズム2014

    • Author(s)
      中口義次
    • Organizer
      第13回石川県立大学食品科学公開セミナー
    • Place of Presentation
      石川県立大学(石川県野々市市)
    • Year and Date
      2014-11-07
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Confirmation of the Excellence of the LAMP-IMS-combined Detection Method for Enterohemorrhagic Escherichia coli and of the Modified System Using a Dried Reagent Mix2014

    • Author(s)
      1)Ahmad Yaman Kayali, Oscar R. Escalante-Maldonado, Kensuke Ozawa, Fumio Gondaira, Akira Ohshima, Varaporn Vuddhakul, Yoshitsugu Nakaguchi, and Mitsuaki Nishibuchi
    • Organizer
      日本防菌防黴学会第41回年次大会
    • Place of Presentation
      きゅりあん(東京都品川区)
    • Year and Date
      2014-09-24 – 2014-09-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Improvement of the Immunomagnetic Separation (IMS)- and Loop-mediated Isothermal Amplification (LAMP)-based Detection Method for Virulent Strains of Vibrio parahaemolyticus from Seafood2014

    • Author(s)
      Oscar R. Escalante-Maldonado, Ahmad Yaman Kayali, Sawa Yamada, Wataru Yamazaki, Fumio Gondaira, Akira Ohshima, Varaporn Vuddhakul, Yoshitsugu Nakaguchi, and Mitsuaki Nishibuchi
    • Organizer
      日本防菌防黴学会第41回年次大会
    • Place of Presentation
      きゅりあん(東京都品川区)
    • Year and Date
      2014-09-24 – 2014-09-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] 変容するアジア地域の食と感染症2014

    • Author(s)
      中口義次
    • Organizer
      平成26年度第2回石川県食品技術研究者ネットワークオープンセミナー
    • Place of Presentation
      石川県工業試験場(石川県金沢市)
    • Year and Date
      2014-08-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] グローバル化する魚介類とローカルな水産発酵食品の細菌性食中毒に対する安全性2014

    • Author(s)
      中口義次
    • Organizer
      石川県立大学シーズ発表会・第17回i-BIRDマッチングセミナー
    • Place of Presentation
      いしかわ大学連携インキュベータ(i-BIRD)(石川県野々市市)
    • Year and Date
      2014-08-02
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 変容する東南アジアでの食と感染症:魚介類と腸炎ビブリオ食中毒2014

    • Author(s)
      中口 義次
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Significance of the trh gene sequence variation in environmental isolates of Vibrio parahaemolyticus2014

    • Author(s)
      Oscar R. Escalante-Maldonado, Ahmad Yaman Kayali, Yoshito Iwade, Wataru Yamazaki, Varaporn Vuddhakul, Yoshitsugu Nakaguchi, Mitsuaki Nishibuchi
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] LAMP-IMS-combined detection method for internationally important enterohemorrhagic Escherichia coli2014

    • Author(s)
      Ahmad Yaman Kayali, Oscar R. Escalante-Maldonado, Fumio Gondaira, Akira Ohshima, Varaporn Vuddhakul, Yoshitsugu Nakaguchi, Mitsuaki Nishibuchi
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 腸炎ビブリオのCaco-2上皮細胞モノレイヤー透過活性と保有病原遺伝子の関連性2014

    • Author(s)
      末澤 千草,中口 義次,西渕 光昭,奥田 潤
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 東南アジアのタイにおける魚介類:養殖二枚貝の生産流通消費と腸炎ビブリオ汚染2013

    • Author(s)
      中口義次、西渕光昭
    • Organizer
      日本防菌防黴学会第40回年次大会
    • Place of Presentation
      千里ライフサイエンスセンター(大阪府豊中市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 市販二枚貝中の腸炎ビブリオ病原性菌株検出の簡便性と感度の改良:免疫磁気ビーズ法およびLAMP法の導入2013

    • Author(s)
      岩出義人、田中夏子、大久保和洋、権平文夫、中口義次、西渕光昭
    • Organizer
      日本防菌防黴学会第40回年次大会
    • Place of Presentation
      千里ライフサイエンスセンター(大阪府豊中市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 塩分濃度を指標としたイカ塩辛における細菌汚染のリスク評価2013

    • Author(s)
      中口義次
    • Organizer
      平成25年度公益社団法人日本水産学会秋季大会
    • Place of Presentation
      三重大学(三重県津市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 東南アジアのタイにおける養殖二枚貝と腸炎ビブリオ汚染2013

    • Author(s)
      中口義次、西渕光昭
    • Organizer
      第34回日本食品微生物学会学術総会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] インドネシアにおける腸炎ビブリオ感染症の発生とその地域間比較2013

    • Author(s)
      中口義次、Abdul Aziz Djamal、Harry Fajri Zisoni、清水理香、勢戸和子、西渕光昭
    • Organizer
      第54回日本熱帯医学会大会
    • Place of Presentation
      長崎ブリックホール(長崎県長崎市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Significance of the trh gene nucleotide sequence variation and its relationship between environmental and clinical isolates of Vibrio parahaemolyticus2013

    • Author(s)
      Oscar R Escalante-Maldonado, Ahmad Yaman Kayali, Yoshito Iwade, Wataru Yamazaki, Varaporn Vuddhakul, Yoshitsugu Nakaguchi, Mitsuaki Nishibuchi
    • Organizer
      第47回腸炎ビブリオシンポジウム
    • Place of Presentation
      広島大学東広島キャンパス(広島県東広島市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development of the method incorporating immunomagnetic separation and loop-mediated isothermal amplification of DNA for detection of internationally important O serotypes of enterohemorrhagic Escherichia coli2013

    • Author(s)
      Ahmed Yaman Kaykli, Oscar R Escalante-Maldonado, Fumio Gondaria, Akira Ohshima, Varaporn Vuddhakul, Yoshitsugu Nakaguchi, Mitsuaki Nishibuchi
    • Organizer
      第47回腸炎ビブリオシンポジウム
    • Place of Presentation
      広島大学東広島キャンパス(広島県東広島市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 東南アジア島嶼部インドネシアにおける腸炎ビブリオ感染症の発生と社会科学的要因との関連

    • Author(s)
      中口義次、Abdul Aziz Djamal、Harry Fajri Zisoni、清水理香、勢戸和子、西渕光昭
    • Organizer
      京都大学微生物科学寄附研究部門主催 第二回シンポジウム
    • Place of Presentation
      京都大学農学部(京都府京都市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] A Loop-mediated Isothermal Amplification (LAMP) Detection Method Based on New tdh-trh Primers and Dried Reaction Mix Are Highly Reliable in Detecting Virulent Strains of Vibrio parahaemolyticus from Seafood

    • Author(s)
      Oscar R. Escalante-Maldonado, Ahmad Yaman Kayali, Wataru Yamazaki, Yoshitsugu Nakaguchi, Mitsuaki Nishibuchi
    • Organizer
      京都大学微生物科学寄附研究部門主催 第二回シンポジウム
    • Place of Presentation
      京都大学農学部(京都府京都市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Nucleotide sequence variation in the trh gene of Vibrio parahaemolyticus environmental isolates and its relationship with that of clinical isolates

    • Author(s)
      Oscar R. Escalante-Maldonado, Ahmad Yaman Kayali, Yoshito Iwade, Wataru Yamazaki, Varaporn Vuddhakul, Yoshitsugu Nakaguchi, and Mitsuaki Nishibuchi
    • Organizer
      第66回日本細菌学会関西支部総会
    • Place of Presentation
      大阪大学微生物病研究所融合生命科学総合研究棟谷口記念講堂(大阪府豊中市)
    • Related Report
      2013 Annual Research Report
  • [Book] 腸炎ビブリオ第IV集2013

    • Author(s)
      中口義次、他
    • Total Pages
      376
    • Publisher
      近代出版
    • Related Report
      2013 Annual Research Report
  • [Funded Workshop] International workshop: Detection of Enterohaemorrhagic E. coli on beef and fecal diarrheal samples with LAMP, IMS and PCR method2016

    • Place of Presentation
      Andalas University, Padang, Indonesia
    • Year and Date
      2016-09-13
    • Related Report
      2016 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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