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Gap junction intercellular communications in tenocyte inflammatory responses to mechanical and thermal stimuli

Research Project

Project/Area Number 25702022
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionHokkaido University

Principal Investigator

Maeda Eijiro  北海道大学, 工学(系)研究科(研究院), 助教 (20581614)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
Keywords腱 / 炎症 / ギャップ結合 / 温度 / 拡散係数 / 物質輸送 / バイオメカニクス / 細胞間情報伝達 / 腱細胞 / 力学刺激 / 熱刺激 / 腱炎 / FLIP / メカノバイオロジー / ギャップジャンクション
Outline of Final Research Achievements

The present study was performed to investigate tenocyte responses to severe mechanical and thermal stimuli and the contributions of gap junction intercellular communications (GJIC) to these responses. When tenocytes were subjected to heat stimulus at 43 °C for 30 minutes, there were significant increases in the expressions of genes related to inflammatory responses. In addition, these responses were further upregulated by the chemical inhibition of GJIC. Accordingly, the presence of GJIC may have an anti-inflammatory effect on tenocyte functions. In the case of the application of static tensile strain to tenocytes, there was an amplitude-dependent regulation of GJIC. The level of GJIC, evaluated as diffusion coefficient, was stably enhanced when tenocytes were subjected to physiological 4% static strain, whereas it became unstable when subjected to non-physiological 8% static strain.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (18 results)

All 2016 2015 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (17 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results)

  • [Journal Article] Mechano-regulation of gap junction communications between tendon cells is dependent on the magnitude of tensile strain2015

    • Author(s)
      Eijiro Maeda and Toshiro Ohashi
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 465 Issue: 2 Pages: 281-286

    • DOI

      10.1016/j.bbrc.2015.08.021

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 腱細胞への温熱刺激による炎症性応答における細胞間物質輸送の寄与2016

    • Author(s)
      前田英次郎,田代真啓,大橋俊朗
    • Organizer
      第28回バイオエンジニアリング講演会
    • Place of Presentation
      東京工業大学(東京都目黒区)
    • Year and Date
      2016-01-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 引張り負荷下の腱細胞間ギャップ結合コミュニケーションの経時変化の解析2016

    • Author(s)
      片海丞,前田英次郎,大橋俊朗
    • Organizer
      第28回バイオエンジニアリング講演会
    • Place of Presentation
      東京工業大学(東京都目黒区)
    • Year and Date
      2016-01-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] Cyclic tensile strain regulates gap junction intercellular communication between tenocytes in vitro in a strain magnitude-dependent manner2015

    • Author(s)
      Hai Cheng Pian, Naoya Kurihara, Eijiro Maeda, Toshiro Ohashi
    • Organizer
      The 8th Asian-Pacific Conference on Biomechanics
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2015-09-17 – 2015-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Cyclic tensile strain regulates gap junction intercellular communication between tenocytes in vitro in a strain magnitude-dependent manner2015

    • Author(s)
      H.C. Pian, N. Kurihara, E. Maeda, T. Ohashi
    • Organizer
      8th Asian Pacific Conference on Biomechanics
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2015-09-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Temperature rise causes upregulation of tenocyte catabolism and enhances gap junctional intercellular communications2015

    • Author(s)
      Masataka Tashiro, Eijiro Maeda, Toshiro Ohashi
    • Organizer
      Summer Biomechanics, Bioengineering and Biotransport Conference
    • Place of Presentation
      Snowbird Resort, Utah, USA
    • Year and Date
      2015-06-17 – 2015-06-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] Temperature rise causes upregulation of tenocyte catabolism and enhances gap junctional intercellular communications2015

    • Author(s)
      M. Tashiro, E. Maeda, T. Ohashi,
    • Organizer
      2015 Summer Biomechanics, Bioengineering and Biotransport Conference
    • Place of Presentation
      スノーバード(アメリカ)
    • Year and Date
      2015-06-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 高温環境での腱細胞異化作用と細胞間情報伝達の変化2015

    • Author(s)
      田代真啓,前田英次郎,大橋俊朗
    • Organizer
      第38回バイオレオロジー学会年会
    • Place of Presentation
      国際学術センター(東京都千代田区)
    • Year and Date
      2015-06-06 – 2015-06-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] Evaluation of intercellular diffusivity of hyperthermia-exposed tenocytes by using FLIP experiment2015

    • Author(s)
      Toshiro Ohashi, Masataka Tashiro, Eijiro Maeda
    • Organizer
      第54回日本生体医工学会大会
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Year and Date
      2015-05-07 – 2015-05-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] 高温環境が腱細胞の異化作用および細胞間物質輸送に与える影響2015

    • Author(s)
      田代真啓,前田英次郎,大橋俊朗
    • Organizer
      第27回バイオエンジニアリング講演会
    • Place of Presentation
      朱鷺メッセ(新潟市)
    • Year and Date
      2015-01-09 – 2015-01-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] 高温環境が腱細胞に及ぼす影響の検討2014

    • Author(s)
      田代真啓,前田英次郎,大橋俊朗
    • Organizer
      LIFE2014 (生活生命支援医療福祉工学系学会連合大会2014)
    • Place of Presentation
      ルスツリゾート(留寿都市)
    • Year and Date
      2014-09-24 – 2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] FLIP法を用いた腱細胞間シグナル伝達に及ぼす力学負荷の影響2014

    • Author(s)
      前田英次郎,大橋俊朗
    • Organizer
      日本機械学会2014年度年次大会
    • Place of Presentation
      東京電機大学(東京都足立区)
    • Year and Date
      2014-09-07 – 2014-09-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] Effect of Hyperthermia on Tenocyte Physiological Function2014

    • Author(s)
      Masataka Tashiro, Eijiro Maeda, Toshiro Ohashi
    • Organizer
      International Scientific Tendinopathy Symposium
    • Place of Presentation
      University of Oxford, Oxford, UK
    • Year and Date
      2014-09-05 – 2014-09-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Evaluation of intra- and inter-cellular diffusivity in tenocytes cultured in row subjected to application of mechanical stretching2014

    • Author(s)
      Eijiro Maeda, Toshiro Ohashi
    • Organizer
      The Fourth Japan-Switzerland Workshop on Biomechanics
    • Place of Presentation
      志摩観光ホテル(志摩市)
    • Year and Date
      2014-09-01 – 2014-09-04
    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
  • [Presentation] FLIP法を用いた細胞内・細胞間物質輸送現象の評価2014

    • Author(s)
      前田英次郎,大橋俊朗
    • Organizer
      第42回可視化情報シンポジウム
    • Place of Presentation
      工学院大学(東京都新宿区)
    • Year and Date
      2014-07-21 – 2014-07-22
    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Invited
  • [Presentation] Evaluation of intra- and inter-cellular diffusivity in tenocytes cultured in row subjected to application of mechanical stretching2014

    • Author(s)
      Eijiro Maeda, Toshiro Ohashi
    • Organizer
      International Symposium on Mechanobiology 2014
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2014-05-20 – 2015-05-23
    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
  • [Presentation] FLIP法を用いた腱細胞間シグナル伝達に及ぼす力学負荷の影響2014

    • Author(s)
      前田英次郎,大橋俊朗
    • Organizer
      日本機械学会 2014年度年次大会
    • Place of Presentation
      東京電機大学(東京都足立区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細胞内外にある流れ:メカノバイオロジーの観点から2013

    • Author(s)
      前田英次郎
    • Organizer
      第17回オーガナイズド混相流フォーラム
    • Place of Presentation
      国民宿舎サンライズ九十九里(千葉県山武郡九十九里町)
    • Related Report
      2013 Annual Research Report
    • Invited

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Published: 2013-05-21   Modified: 2019-07-29  

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