| Budget Amount *help |
¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
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| Outline of Final Research Achievements |
The present study was performed to investigate tenocyte responses to severe mechanical and thermal stimuli and the contributions of gap junction intercellular communications (GJIC) to these responses. When tenocytes were subjected to heat stimulus at 43 °C for 30 minutes, there were significant increases in the expressions of genes related to inflammatory responses. In addition, these responses were further upregulated by the chemical inhibition of GJIC. Accordingly, the presence of GJIC may have an anti-inflammatory effect on tenocyte functions. In the case of the application of static tensile strain to tenocytes, there was an amplitude-dependent regulation of GJIC. The level of GJIC, evaluated as diffusion coefficient, was stably enhanced when tenocytes were subjected to physiological 4% static strain, whereas it became unstable when subjected to non-physiological 8% static strain.
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