Ultrafast optical microscopy for detection of circulating tumor cells in blood
| Project/Area Number |
25702024
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | The University of Tokyo |
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Principal Investigator |
GODA Kisuke 東京大学, 理学(系)研究科(研究院), 教授 (70518696)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
Fiscal Year 2014: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2013: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
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| Keywords | 超高速イメージング / がん / フローサイトメトリー / 循環腫瘍細胞 / 高速顕微鏡 / マイクロ流体工学 / ガン / マイクロ流体 / 分析化学 / 高速イメージング / CTC |
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| Outline of Final Research Achievements |
We developed an ultrafast optical microscope for early, noninvasive, low-cost detection of cancer. This microscope is based on a combination of an ultrafast optical imager known as STEAM and a multi-color fluorescence analyzer for high-throughput screening of cells in a large heterogeneous population. Based on a Ti:Sapphire femtosecond pulse laser, we achieved high spatial resolution of 780 nm and high frame rate of 70 Mfps for high-speed morphological screening. Furthermore, we achieved focusing and ordering of cells in high-speed flow with high precision of a few micrometers. Using the developed system, we demonstrated single-cell analysis of a large population of cells with a high throughput of 10,000 cells/s. This work has been absorbed into the ImPACT program in which a far more advanced system is currently under development.
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Report
(3 results)
Research Products
(6 results)
