Identification and development of therapeutic strategy for treatment of skeletal muscle loss and weakness "MYOPENIA"

• Project/Area Number: 25702041
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Sports science
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: Miyazaki Mitsunori 北海道医療大学, リハビリテーション科学部, 准教授 (20632467)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000); Fiscal Year 2013: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
• Keywords: 運動生理学 / 骨格筋 / 悪液質 / マイオペニア / 細胞内シグナル伝達 / スポーツ生理学 / スポーツ生化学 / タンパク質代謝 / 骨格筋肥大 / 骨格筋萎縮 / 身体運動 / カケキシア / 筋肥大 / 筋萎縮 / サルコペニア / 細胞内シグナル伝達系

Outline of Final Research Achievements

This research project was conducted to Identify and develop therapeutic strategies for treatment of skeletal muscle loss and weakness termed as "MYOPIA". Myopenia is generally developed under several physiological and pathological conditions including aging, hypomobility and wasting diseases such as AIDS/COPD/cancer.
Main achievements of this research project were summarized as follows. 1) Identification of intracellular signaling mechanisms regulating skeletal muscle size. 2) Comprehensive data analyses of gene expression in skeletal muscle under myopenic condition. 3) Development of the therapeutic interventions for myopenia using whole-body exercise.

Research Products

• [Journal Article] TSC2/Rheb signaling mediates ERK-dependent regulation of mTORC1 activity in C2C12 (2017)
  • Author(s): Mitsunori Miyazaki and Tohru Takemasa
  • Journal Title: FEBS Open Bio Volume: 7 Issue: 3 Pages: 424-433
  • DOI: 10.1002/2211-5463.12195
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 冬眠中のクマはなぜ寝たきりにならないのか？ (2016)
  • Author(s): 宮崎充功
  • Journal Title: BEARS JAPAN Volume: 16 Pages: 26-27
• [Journal Article] Altered signaling pathway governing protein metabolism in skeletal muscle of the Japanese black bear during hibernation (2015)
  • Author(s): Mitsunori Miyazaki, Michito Shimozuru and Toshio Tsubota
  • Journal Title: THE FASEB JOURNAL Volume: 29
• [Journal Article] Growth factor-dependent and independent regulation of skeletal muscle mass - Is IGF-1 necessary for skeletal muscle hypertrophy? - (2013)
  • Author(s): Mitsunori Miyazaki
  • Journal Title: The Journal of Physical Fitness and Sports Medicine Volume: 2 Issue: 1 Pages: 101-106
  • DOI: 10.7600/jpfsm.2.101
  • NAID: 10031159022
  • ISSN: 2186-8123, 2186-8131
  • Peer Reviewed
• [Presentation] 骨格筋肥大適応における Akt1 依存性／非依存性の制御機構 (2017)
  • Author(s): 宮崎充功
  • Organizer: 第5回骨格筋生物学研究会
  • Place of Presentation: 東京大学駒場キャンパス（東京都目黒区）
  • Year and Date: 2017-03-03
• [Presentation] Akt1遺伝子機能欠損モデルにおける骨格筋mTORの活性化 (2016)
  • Author(s): 宮崎充功、森谷伸樹
  • Organizer: 第71回日本体力医学会大会
  • Place of Presentation: 盛岡市民文化ホール／いわて県民交流センター（岩手県盛岡市）
  • Year and Date: 2016-09-23
• [Presentation] Regulation of protein metabolism and muscle mass in hibernating bears: an attractive model of muscle atrophy resistance (2016)
  • Author(s): Mitsunori Myazaki, Michito Shimozuru and Toshio Tsubota
  • Organizer: 24th International Conference on Bear Research and Management
  • Place of Presentation: Alaska, USA
  • Year and Date: 2016-06-12
  • Int'l Joint Research / Invited
• [Presentation] Changes in energy metabolism in hibernating black bears: an analysis of gene expression and metabolites in liver and skeletal muscle (2016)
  • Author(s): Michito Shimozuru, Mitsunori Miyazaki and Toshio Tsubota
  • Organizer: 24th International Conference on Bear Research and Management
  • Place of Presentation: Alaska, USA
  • Year and Date: 2016-06-12
  • Int'l Joint Research / Invited
• [Presentation] Regulation of protein metabolism and muscle mass in hibernating bears: an attractive model of muscle atrophy resistance. (2016)
  • Author(s): Mitsunori Miyazaki, Michito Shimozuru and Toshio Tsubota
  • Organizer: 24th International Conference on Bear Research and Management
  • Place of Presentation: Anchorage, AK, USA
  • Year and Date: 2016-06-12
  • Int'l Joint Research / Invited
• [Presentation] Biological Strategy for Muscle Mass Reservation in Hibernating Animals (2016)
  • Author(s): 宮崎充功
  • Organizer: 第4回 骨格筋生物学研究会
  • Place of Presentation: 松本大学、松本市
  • Year and Date: 2016-03-04
• [Presentation] 癌性カケキシアに伴う骨格筋の遺伝子発現変化とタンパク質代謝制御 (2015)
  • Author(s): 宮崎充功
  • Organizer: 第2回日本基礎理学療法学会
  • Place of Presentation: 神奈川県立保健福祉大学、横須賀市
  • Year and Date: 2015-11-14
• [Presentation] 骨格筋肥大適応におけるmTOR活性の制御機構 ～Akt1 knockoutマウスを用いた検討～ (2015)
  • Author(s): 森谷伸樹、宮崎充功
  • Organizer: 第2回日本基礎理学療法学会
  • Place of Presentation: 神奈川県立保健福祉大学、横須賀市
  • Year and Date: 2015-11-14
• [Presentation] 癌性悪液質に伴う骨格筋タンパク質代謝能の変化 (2015)
  • Author(s): 宮崎充功
  • Organizer: 第2回日本サルコペニア・フレイル研究会
  • Place of Presentation: 東京大学、東京
  • Year and Date: 2015-10-04
• [Presentation] 癌性カケキシアによる運動誘発性骨格筋タンパク質合成能の低下 (2015)
  • Author(s): 宮崎充功
  • Organizer: 第70回日本体力医学会大会
  • Place of Presentation: 和歌山県民文化会館、和歌山市
  • Year and Date: 2015-09-18
• [Presentation] Hibernating bear muscle shows slow-fiber shifting and mitochondrial biogenesis despite prolonged physical inactivity (2015)
  • Author(s): Mitsunori Miyazaki, Michito Shimozuru and Toshio Tsubota
  • Organizer: Cell Symposia: Exercise Metabolism
  • Place of Presentation: NH Grand Hotel Krasnapolsky, Amsterdam
  • Year and Date: 2015-07-12 – 2015-07-14
• [Presentation] Hibernating bear muscle shows slow-fiber shifting and mitochondrial biogenesis despite prolonged physical inactivity (2015)
  • Author(s): Mitsunori Miyazaki, Michito Shimozuru and Toshio Tsubota
  • Organizer: Cell Symposia: Exercise Metabolism
  • Place of Presentation: Amsterdam, The Netherlands
  • Year and Date: 2015-07-12
  • Int'l Joint Research
• [Presentation] Altered signaling pathway governing protein metabolism in skeletal muscle of the Japanese black bear during hibernation (2015)
  • Author(s): Mitsunori Miyazaki, Michito Shimozuru and Toshio Tsubota
  • Organizer: Experimental Biology 2015
  • Place of Presentation: Boston, MA, USA
  • Year and Date: 2015-04-01
  • Int'l Joint Research
• [Presentation] Altered signaling pathway governing protein metabolism in skeletal muscle of the Japanese black bear during hibernation (2015)
  • Author(s): Mitsunori Miyazaki, Michito Shimozuru and Toshio Tsubota
  • Organizer: Experimental Biology 2015
  • Place of Presentation: Boston Convention and Exhibition Center, Boston
  • Year and Date: 2015-03-28 – 2015-04-01
• [Presentation] 癌性カケキシアによる骨格筋トレーナビリティの低下 (2015)
  • Author(s): 宮崎充功
  • Organizer: 第３回骨格筋生物学研究会
  • Place of Presentation: 東北大学、仙台市
  • Year and Date: 2015-03-06 – 2015-03-08
• [Presentation] 骨格筋量を保持するための新奇ストラテジー探索 (2014)
  • Author(s): 宮崎充功
  • Organizer: 日本体力医学会
  • Place of Presentation: 長崎大学、長崎市
  • Year and Date: 2014-09-18 – 2014-09-20
  • Invited
• [Presentation] 冬眠動物はなぜ寝たきりにならないのか？-筋萎縮耐性獲得の分子基盤探索- (2014)
  • Author(s): 宮崎充功
  • Organizer: 第２回骨格筋生物学研究会
  • Place of Presentation: 札幌
• [Presentation] PKC-dependent regulation of mTOR activity is mediated through TSC2/Rheb signaling in C2C12 myoblasts (2013)
  • Author(s): Mitsunori Miyazaki, Tohru Takemasa
  • Organizer: IUPS2013 (37th Congress of the International Union of Physiological Sciences)
  • Place of Presentation: Birmingham, UK
• [Presentation] MEK/ ERK-dependent regulation of mTOR activity is mediated through TSC2/Rheb signaling in C2C12 myoblasts (2013)
  • Author(s): Mitsunori Miyazaki
  • Organizer: 7th Cachexia Conference
  • Place of Presentation: Kobe, Japan
• [Presentation] 高脂肪食摂取による肥満症の発症と末梢組織における時計遺伝子発現パターンの変化 (2013)
  • Author(s): 宮崎充功、武政徹
  • Organizer: 第68回日本体力医学会大会
  • Place of Presentation: 東京都
• [Presentation] Bear foot -animal study- 野生動物から学ぶ身体健康科学 (2013)
  • Author(s): 宮崎充功
  • Organizer: スポーツ科学未来研究所シンポジウム
  • Place of Presentation: 早稲田大学大隈記念タワー
  • Invited
• [Book] Basic Biology and Current Understanding of Skeletal Muscle (2013)
  • Author(s): Mitsunori Miyazaki
  • Total Pages: 318
  • Publisher: Nova Science Publishers